Background. A large number of longitudinal studies indicate significantly increased risk of cardiovascular events and death in people with the MetSyn and high plasma levels of triglycerides are an independent risk factor for the development of cardiovascular disease. Apolipoprotein A5 (APOA5) gene, a new member of the APOA1/C3/A4 gene cluster, was identified by comparative sequencing of human and mice DNA by Pennacchio and co-workers in 2001. Since this discovery, variants of ApoA5 gene have been independently assiociated with level of plasma triglyceride in many countries. Human ApoA5 is expressed in the liver then appears in plasma in association with VLDL and HDL and plays a major role in TG catabolism. Variant at ApoA5 gene locus, 1177C>T is located in 3’ UTR which often contains regulatory regions that influence post-transcriptional gene expression. One alteration can be responsible for the altered expression of many genes. Materials and Methods. 152 people with MS for case group and 152 people for control group were selected in this study. MS was diagnosed according to IDF criteria and serum triglyceride levels were determined. DNA from both case and control subjects were extracted from blood samples (200 ml) using “G-spin™ Total DNA Extraction Kit”(iNtRON Biotechnology, Inc). To detect the 1177C>T variation of ApoA5 gene, using High Pure PCR Template Preparation Kits, a forward primer 5’-CTCTGAGCCTCTAGCATGGTTGAGT- 3’ and the mismatch reverse primer 5’-GAGCATTCCCAAATGAGCAC-3’ were used to create the HinfI restriction site. Results. There were 304 total subjects included males 50.3% (153) and female 49.7% (151) in our study. Incident of CC genotype was 71.1% (216), CT genotype was 25% (76) and TT genotype was 3.9%, TAG level was higher in males than females in both groups (p=0.016, ð=0.001) for CC genotype and also, higher with MS in males for CT genotype (p=). But, TAG level was no significant difference among three genotypes in group with MS subjects (male p=0.236, female p=0.881). Conclusion: The TT genotype of the ApoA5 gene 1177C>T polymorphism frequency was 2.9% in control subjects and 4.9% in subjects with MS. However, TG level was not differ in both groups for TT genotype, TAG level in males was higher compared with females (p=0.016 in control, p=0.001 in group with MS).

BACKGROUND: According to WHO, Non-communicable disease take place 43% of morbidity as well as 63% of mortality in worldwide and it will may increased as 60% of morbidity, 73% of mortality in 2020. Therefore one quarter of NCD associated with composition and usage of foods. Obesity and Diabetes mellitus are caused by accumulating saturated fat in body due to excessive use of high calorie. High-fat diet promotes lipid deposition in solid organs including skeletal muscle which is underlying cause of insulin resistance. METHODS: Same aged, Inbred (C57BL/6J) 20 male mice are involved in this study. They are raised and fed in central core laboratory of MNUMS. Feeding of mice “Beijing Branch of the Joint Feed Co LTD” from Republic of China produces forage for laboratory animals. They were divided into two groups. A control group mice had been fed standard chow while experimental group mice had been fed 45% high fat diet for 4 weeks. The morphological effect of the a high fat diet was examined by light microscopy. RESULTS: Liver of experimental mice was seen pale and parenchyma had a fatty changes compared to control group liver in gross anatomy. Periportal necrosis and lipid droplet deposition in hepatocytes of study group were detected as microscopically. There is fatty accumulation between solid tissue of pancreas and islets of Langerhans get smaller in study group. Experimental mice which were fed by 45% of fat containing food had lipid droplets were observed in fibers. Therefore degenerated fibers showing separation from the basement membrane and acidophilic staining were observed in skeletal muscle.

: In enterohepatic recycling, foreign chemicals entering the alimentary tract and gut normal micro flora (ShagjJambal., 1989) are absorbed into portal venous blood by enterocytes, removed from blood by uptake into hepatocytes, secreted into the bile, and then deposited back into the intestinal lumen where they may be reabsorbed by intestinal wall cells and available for recycling (compartment model modified by Wang and Reuning). This process is called enterohepatic circulation or enterohepatic recycling (Rollins and Klaasen, 1979). Some studies have attempted to interrupt the enterohepatic recycling of toxicants such as pesticides and heavy metals and 56 carbon tetrachloride (CCl₄). Plant Material The plant Gentiana macrophylla pall. Was collected during july 2017 from the forest region Galt sum, Khuvsgul aimakh, Mongolian. The taxonomical identification of the plant was done by Botanical survey of Ganbold E. (ScD) Mongolian university subsidiary of Ulanbator institute, Ulanbator. The study was based on the New medicine medical university bio-model, pharmacology laboratory and laboratory. ELISA-biochemistry laboratory. Animals: Male C57BL/6 (miceweight 18-22g (n=40)) were obtained from the Mongolian national university of Medical sciences animal house. Experimental procuders were conducted in accordance with the regulations of Animal Ethical committee. Myeloperoxidase (MPO) activity was measured according to the technique described by Krawisz et al. (1984) . Assay for lipid peroxides in animal tissues by thiobarbituric acid Reaction (Yagi, K. and Ohkova.,1979). A significant decrease (P<0.005) in the MDA was observed in the Gentiana macrophylla(GM) treated group (0.340±0.031)when compared with the control group (0.521±0.023). Conclusion The results of the study show that Gentiana macrophylla is inhibition of lipid peroxidation, protection of liver cell. From the protonation compounds generate polyphenol compound with rich hydroxyl and alkaloid. These compounds help to lipid peroxidation or antioxidation and membrane protection.The results of the study show that Gentiana macrophylla is inhibition off oxidation ferric molecule in vitro reaction and detoxification of reactive oxygen species release from damaged liver cell by CCL₄.

Background: Medicinal herbs have been used in traditional medicine to treat systemic inflammatory disease for many years. For instance, Rhodiola rosea L extracts were used to enhance behavioural stresses for improving fatigue and depression. Gallic acid, found in Rhodiola rosea L and Rhodiola quidrifida is, a natural polyphenol, exhibits multiple therapeutic activities, including anti-inflammatory, anti-cancer, antioxidant, and anti-angiogenic effects. Saposhnikovia divaricate (its ethanol extract mainly) notified to support function of musculoskeletal tissue and to enhance tissue regeneration by its anti-inflammatory effect. The Salsola laricifolia L has been studied for its strong antioxidant activity, improve immune function, boost energy, and exhibit anti-inflammatory effects. This study allowed us to screen anti-inflammatory effects of medicinal plants and future therapeutic possibility. Aim: To study the content of phenolic compounds (gallic acid) in the composition of complex products of plants widely used in Mongolian traditional medicine, which have been found to have biological activity of the extracts Materials and Methods: The herbal extract was extracted from Rhodiola rosea L, Saposhnikovia divaricata (Tuscz) Schischk, Rhodiola quidrifida Pall.Fisch, Salsola laricifolia Turcz.ex Litv in laboratory of the Drug Research Institute of Monos Group, Mongolia. We used solvents for HPLC grade was used to identify the bioactive components that Gallic acid in the compound plant extract. : Results: The bioactive compounds in each of the Rhodiola rosea L, Rhodiola quadrifida Pall.Fisch and four medicinal plant extracts were identified using HPLC, confirming the presence of Gallic acid. Conclusion Our research results showed that the quantitative content of Gallic acid in the composite plant extract was high, at 1.02%.