BACKGROUND: According to WHO, Non-communicable disease take place 43% of morbidity as well as 63% of mortality in worldwide and it will may increased as 60% of morbidity, 73% of mortality in 2020. Therefore one quarter of NCD associated with composition and usage of foods. Obesity and Diabetes mellitus are caused by accumulating saturated fat in body due to excessive use of high calorie. High-fat diet promotes lipid deposition in solid organs including skeletal muscle which is underlying cause of insulin resistance. METHODS: Same aged, Inbred (C57BL/6J) 20 male mice are involved in this study. They are raised and fed in central core laboratory of MNUMS. Feeding of mice “Beijing Branch of the Joint Feed Co LTD” from Republic of China produces forage for laboratory animals. They were divided into two groups. A control group mice had been fed standard chow while experimental group mice had been fed 45% high fat diet for 4 weeks. The morphological effect of the a high fat diet was examined by light microscopy. RESULTS: Liver of experimental mice was seen pale and parenchyma had a fatty changes compared to control group liver in gross anatomy. Periportal necrosis and lipid droplet deposition in hepatocytes of study group were detected as microscopically. There is fatty accumulation between solid tissue of pancreas and islets of Langerhans get smaller in study group. Experimental mice which were fed by 45% of fat containing food had lipid droplets were observed in fibers. Therefore degenerated fibers showing separation from the basement membrane and acidophilic staining were observed in skeletal muscle.

Background: Poor laboratory quality can lead to misdiagnosis and inappropriate treatment of patients. To demonstrate the quality and reliability of their services, medical laboratories seek accreditation to ISO 15189. We have initiated a project to assist laboratories in their efforts to obtain the accreditation. Goal: Conduct a gap analysis of the status of preparedness of medical laboratories for accreditation. Materials and Methods: Six laboratories are selected for participation in the project. In the first phase of the project, a gap analysis of the participant laboratories is conducted using an Excel program based on ISO 15189 requirements. Results: The findings reveal that the participant laboratories are the strongest in Organization and management of laboratory, Quality of examination results, Personnel and facility management and in Laboratory information management. The majority of the laboratories are hospital based, and their organization and management are well established and functional mostly due to centralized administrative guidance. The concept of quality control is effectively adapted in medical laboratories, therefore ensuring the quality of examinations and the data management are usually in line with the requirements. Weaker areas include Evaluation and audits, and Document control. Even though the laboratories do conduct evaluations and control, they do not do it regularly and, most importantly, do not keep records routinely, which cause the higher gap rate. Conclusion Policies to meet ISO 15189 requirements are in place in the participant laboratories, but their documentation and records keeping are insufficient.

Introduction: Cancer arises from abnormal cells nonstop divide in an uncontrolled way and without stopping. In GLOBOCAN reported that 14.1 million new cancer cases, 8.2 million cancer deaths and 32.6 million people suffering with cancer in 2012 worldwide. Among the world, a surgical, radiation and chemotherapy maintains the cancer treatment within their combination, and moreover, scientists believe that, in order to enhance the result and come up with better spotlight therapeutic outcome, immunotherapy should be added within combination of surgical, radiation and chemotherapy. Objective: To identify the immune ability of mononuclear cells in peripheral blood, and to study their cytolytic activity on cancer cellular line. Materials and method: Cytolytic activity assay was done with healthy human's PBMCs induced by non specific mytogen phytohemaglutinin L (PHA) of 10ul/ml, 5ul/ml, 2.5ul/ml dosage, and cultured with SP2Myeloma cell line. Then live cells and death cells analyzed with MTT assay and Propidium iodide, respectively, as well as CD+25 cells analyzed with Apogee flow cytometry at 24.48 and 72 hours of uncubation at 5% CO2, 37 C0. Result: Cancer Inhibition rate of mononuclear cells in blood (inhibition rate %) was assessed by МТТ assay and the culture of control group of mononuclear cells and myeloma cells’ median was (OD=0.36±0.05) while Phytohemaglutinin induced group’s result in 72 hours was (OD=0.31±0.03) which suggests that linear cells mononuclear cell’s mixture rate is 86.1%. Dead cell’s number assessed by Propidiumiodid (Cell toxicity assay) shows the result of control group’s cell death (10.68±2.1%), while Phytohemaglutinin induced group it was 20-25% which is statistically significant. (p<0.05). Cancer elimination activity assessed by СD25+ Т cells and the result of CD25+ Т number activated by Phytohemaglutinin (152-282 cell/µl), in control group (26cell/µl) which is statistically significant(p<0.001). Conclusion: 1. Myeloma SP2/0 cell line is inhibited the growth by peripheral mononuclear cells and it is not dependent dosage of Phytohemaglutinin. 2. Death of cell assessed by propidium iodide, control group’s cell death 10.68±2.1%, while Phytohemaglutinin induced group it was 20-25% which is statistically significant (p<0.05). 3. Cancer elimination activation assessed by СD25+ Т cells shows the result of CD25+ Т number activated by Phytohemaglutinin (152-282cell/µl), in control group (26cell/µl) which is statistically significant(p<0.001).

Background: Psychosocial work environment predicts a range of health risks including sickness absence, poor self rated health, and depression. Objective: To identify association between psychosocial work environment and health related indicators among health care workers in Mongolia Methods: A cross-sectional study was conducted between January and April, 2023. 655 health care workers from Ulaanbaatar city and 4 aimags were involved. Psychosocial work environment was assessed by the Effort-reward imbalance model. As for the health related indicators, we used the SF-12 questionnaire and sickness absence report. Descriptive and inferential statistical analyses were performed.
The study was approved by Medical Ethics Review Committee on January 18, 2023 (#23/02). Results: 1 in every 2 health care workers experience effort-reward imbalance at work and rated their health as poor. In average, 32.7 percent of the study participants took 16.6 sick days for the last 1 year. The study identified poor physical (44.7±8.6) and mental health functioning (42.1±9.8) among study participants. Physical functioning was lower among nurses whereas mental health functioning was lower among physicians. Conclusion Poor health related indicators among health care workers are associated with adverse psychosocial work environment such as effort-reward imbalance and high effort and low reward

Background: Medicinal herbs have been used in traditional medicine to treat systemic inflammatory disease for many years. For instance, Rhodiola rosea L extracts were used to enhance behavioural stresses for improving fatigue and depression. Gallic acid, found in Rhodiola rosea L and Rhodiola quidrifida is, a natural polyphenol, exhibits multiple therapeutic activities, including anti-inflammatory, anti-cancer, antioxidant, and anti-angiogenic effects. Saposhnikovia divaricate (its ethanol extract mainly) notified to support function of musculoskeletal tissue and to enhance tissue regeneration by its anti-inflammatory effect. The Salsola laricifolia L has been studied for its strong antioxidant activity, improve immune function, boost energy, and exhibit anti-inflammatory effects. This study allowed us to screen anti-inflammatory effects of medicinal plants and future therapeutic possibility. Aim: To study the content of phenolic compounds (gallic acid) in the composition of complex products of plants widely used in Mongolian traditional medicine, which have been found to have biological activity of the extracts Materials and Methods: The herbal extract was extracted from Rhodiola rosea L, Saposhnikovia divaricata (Tuscz) Schischk, Rhodiola quidrifida Pall.Fisch, Salsola laricifolia Turcz.ex Litv in laboratory of the Drug Research Institute of Monos Group, Mongolia. We used solvents for HPLC grade was used to identify the bioactive components that Gallic acid in the compound plant extract. : Results: The bioactive compounds in each of the Rhodiola rosea L, Rhodiola quadrifida Pall.Fisch and four medicinal plant extracts were identified using HPLC, confirming the presence of Gallic acid. Conclusion Our research results showed that the quantitative content of Gallic acid in the composite plant extract was high, at 1.02%.