The objective of this study is to determine the incidence of microbial contamination on medical students’ white coats, the way they handle and clean their white coats and their perception towards contamination. For this purpose, cross sectional survey of the bacterial contamination of white coats in a medical college has been carried out in 3 different locations; Royal College of Medicine, Perak, University of Kuala Lumpur and a private college attached to Ipoh General Hospital. It was found that the incidence of Staphylococus aureus, was 32% on short-sleeved and 54% on long-sleeved white coats. Bacillus species was the second most common type of bacteria found. Male collars and female pockets had higher microbial contaminations (p=0.01, 0.03 respectively). Clinical students’ white coats were significantly less contaminated than non-clinical students (p=0.001) although they tend to wear it for a longer period (5.75 ± 2.19 h vs. 2.32 ± 0.81 h) (p=0.001). Clinical students owned more short-sleeved coats (p=0.001) and washed their coats more often (p=0.01) than non-clinical ones. More than eighty one percent of clinical students wear their white coats in the college the majority of whom were females (p=0.005). Perception of clinical and non-clinical students towards white coat contamination was similar. Medical students’ white coats are contaminated with bacteria and they are potentially source of cross infection. Student’s way of handling and washing white coats should be corrected by issuing and following standard guidelines. Students should be bared from wearing white coats in non-clinical areas. Washing hands and using plastic aprons is highly recommended before examining wounds