In order to studying the action mechanism of moxibustion from a viewpoint of the sexual periodicity, an experiment was attempted to ascertain whether the irregular estrous cycles were able to recover the regular cyclicity by preiodic moxibustion in rats ovariectomized and transplanted subcutaneously with the ovaries.
From the day of operation, two groups of the rats were given every four days subcutaneous injections of 2.0mg of progesterone, and 0.5mg of reserpine, respectively, for 40 days. one group of the rats were used as moxibustion animals. 5mg of moxa was every four days divided into 6 cones and they were treated by each 2 cones at right GB-25, left GB-25 and GV-20, respectively, for 40 days. Vaginal smears were recorded every day during the treatment period and subsequent 40 days. It is suggested that an isochronal 4-day rhythm appears to be established as early as about 10 to 20 days after the start of the moxibustion receiving animals and the results of experiment gave nearly agreement with the value that were given every four days injections of 2mg of progesterone, respectively, for 40 days.

We examined effects of electro-acupuncture on the jaw opening reflex. Using rats anesthetized with pentobarbital sodium (40mg/kg: intraperitoneal) the jaw opening reflex was evoked by electrical stimulation of the dental pulp. The reflex was evoked by electro-myograms were recorded from the digastric muscle, showing di- or triphasic potentials with latency of about 6 msec. The dental pulp stimulation was preceded by single pulse electroacupuncture (EA) of the fore- or hind-leg.
Facilitation followed by inhibition of the jaw opening reflex was induced by the EA. The facilitation lasted for about 20 msec and 30 msec in case of the fore- and hind-leg EAs, respectively. The following inhibition was observed up to about 300 msec and 400 msec after the fore- and hind-le2 EA9, respectively.
It is worthy of note that train pulse EAs diminished inhibition of the jaw opening reflex.

Effects of repetitive electro-acupuncture stimulation on the blink reflex were studied in 23 healthy adults. The blink reflex was elicited by an electrical stimulus of the supraorbital nerve. The reflex EMG's were recorded from the orbicularis oculi muscle. The EMG's were composed of the early component (R1), ipsilaterel to the supraorbital stimulation, with about 10m sec latency and the bilateral late ones (R2) with about 30m sec latency. Electro-acupuncture stimulations consisting of single pulse and a train pulse were given to the skin on “Hogu” point, respectively.
Single pulse electro-acupuncture stimulation inhibited the R2 components for 30m sec to 1 sec after the stimulation and its inhibitory effect reached maximum about 100m sec after it. The R2 components were also suppressed when the skin was stimulated by a train of ten pulses with frequency of 2c/s to 20c/s, but the inhibitory effect decreased significantly compared with that of single pulse stimulation. The inhibitory effect was also diminished by 10 to 200 pulses electro-acupuncture stimulation with a frequency of 2c/s.

Acupuncture effects are frequently modified by additional acupuncture stimulations. In this study we tried to clarify the mechanisms of such phenomena using the rat jaw opening reflex. The reflex response was elicited by an electrical stimulus of the tooth pulp. The reflex EMG's were recorded from the digastric muscles. Single electroacupuncture stimulation (CS1) was delivered to the skin of a rat.
CS1 facilitated the reflex for 20ms after the stimulation, and then inhibited it from 40ms to 250ms. When CS1 was preceded by another electroacupuncture stimulation (CS2), the facilitatory effect induced by CS1 was enhanced CS2-CS1 interval being within 10ms. Then the reflex was inhibited when CS2-CS1 interval was 40-150ms. On the other hand CS1's inhibitory effect was suppressed by CS2 and the reflex reappeared while CS2 preceded CS1 at an interval of 100ms to 1.5s.