Aims: Glutamate cysteine ligase (GCL) enzyme is involved in the synthesis of glutathione, which functions as an antioxidant. Polymorphisms in the sequence of amino acids making up the gene GCLC will cause differences in enzyme expression and GCLC activity. Gene expression that is influenced by oxidative stress can be used to measure markers such as F2-isoprostanes. This study aims to examine the association between the polymorphism in the GCLC gene with glutathione plasma level and F2-isoprostanes in contacts of person with infectious tuberculosis (TB). Methodology and results: Samples are taken from the family members of pulmonary TB patients who seeks treatment at the Pulmonary Centre (Lung Health Center for Public = BBKPM) and Policlinic of Dr Wahidin Sudirohusodo Hospital, Makassar. Total of approximately 4 mL of venous blood are taken from each person with pulmonary TB contacts and furtherly analyzed using genomic PCR-RFLP method and ELISA. Our results described that contacts of person with infectious TB for approximately 6 months have polymorphism C/C genotype at 80.3%, C/T of 18.3% and T/T for 1.4% of the total 71 samples with high levels of glutathione from 0.167 to 0.548 mM/mL and F2-isoprostanes level 72.4 - 1343.9 pg/mL. Conclusion, significance and impact of study: There are no significant association between GCLC gene polymorphism with glutathione and F2-isoprostanes levels of individual who had contacted infection TB. In this study the elevation of F2-isoprostanes equal to the decrease levels of glutathione.
Glutamate-Cysteine Ligase

Obesity become worrying problem among adolescents, they who are obesed are 80% have the opportunity to experience obesity as adults and have higher risk of serious diseases such as cardiovascular disease, stroke, diabetes, asthma, and some types of cancer. According to the health profile of Yogyakarta Special Province in 2017 the prevalence of obesity was 19.1% occurs in adolescents. The main purpose of the research is to analyze the direct and indirect effects of gadget usage, sleep habit, stress, and physical activity on obesity. The population in this research was obese junior and senior high school adolescents, totaling 150 persons. Determination of samples is taken by simple random sampling. Independent variables consist of gadget usage, stress level, sleep habit, and physical activity. While the dependent variable is obesity. Bivariate analysis with chi-square test is applied for cathegorical data and independent T-test for numerical data. Multivariate analysis is carried out with path analysis, using the Amos version 22.0 software program. The results of this research found that the conclusions of the path diagram generated are as follow: 1) Gadget usage variable path coefficient value has a positive effect on physical activity. 2) Physical activity variable path coefficient value has a positive in effect on obesity. 3) Sleep habit and stress variable path coefficient value has a positive effect on gadget usage. It can be concluded that gadget addiction is directly related to sleep habits, stress, physical activity, and indirectly with obesity.