1.A novel cost-effective medium for the production of Bacillus thuringiensis subsp. israelensis for mosquito control
Subbiah Poopathi ; Archana, B.
Tropical Biomedicine 2012;29(1):81-91
Abstract. Bacillus thuringiensis subsp. israelensis (Bti) has been used for mosquito-control
programmes the world-wide. Indeed, the large-scale production of Bti for mosquito control is
very expensive due to the high cost of its culture. In the present study, we attempted to widen
the scope in developing cost-effective culture medium for Bti production, based on the raw
materials available on the biosphere, including coconut cake powder, CCP (Cocos nucifera),
neem cake powder, NCP (Azadirachta indica) and groundnut cake powder, GCP (Arachis
hypogea). Among these raw materials, the biomass production of Bti, sporulation and toxin
synthesizing from ‘CCP’ in combination with mineral salt (MnCl2) was comfortably satisfactory.
Bioassays with mosquito species (Culex quinquefasciatus, Anopheles stephensi and Aedes
aegypti) and field trials were also satisfactory. The present investigation suggests that coconut
cake-based culture medium can be used as an alternative for industrial production of Bti in
mosquito-control programme. Therefore, the study is very important from the point of effective
production of Bti from cost-effective culture medium for the control of mosquito vectors.
2.Impaired liver regeneration and lipid homeostasis in CCl4 treated WDR13 deficient mice
Arun Prakash MISHRA ; Archana B. SIVA ; Chandrashekaran GURUNATHAN ; Y. KOMALA ; B. Jyothi LAKSHMI
Laboratory Animal Research 2020;36(4):318-327
WDR13 - a WD repeat protein, is abundant in pancreas, liver, ovary and testis. Absence of this protein in mice has been seen to be associated with pancreatic β-cell proliferation, hyperinsulinemia and age dependent mild obesity. Previously, we have reported that the absence of WDR13 in diabetic Leprdb/db mice helps in amelioration of fatty liver phenotype along with diabetes and systemic inflammation. This intrigued us to study direct liver injury and hepatic regeneration in Wdr13−/0 mice using hepatotoxin CCl4. In the present study we report slower hepatic regeneration in Wdr13−/0 mice as compared to their wild type littermates after CCl4 administration. Interestingly, during the regeneration phase, hepatic hypertriglyceridemia was observed in Wdr13 −/0 mice. Further analyses revealed an upregulation of PPAR pathway in the liver of CCl4- administered Wdr13 −/0 mice, causing de novo lipogenesis. The slower hepatic regeneration observed in CCl4 administered Wdr13 −/0 mice, may be linked to liver hypertriglyceridemia because of activation of PPAR pathway.
3.Impaired liver regeneration and lipid homeostasis in CCl4 treated WDR13 deficient mice
Arun Prakash MISHRA ; Archana B. SIVA ; Chandrashekaran GURUNATHAN ; Y. KOMALA ; B. Jyothi LAKSHMI
Laboratory Animal Research 2020;36(4):318-327
WDR13 - a WD repeat protein, is abundant in pancreas, liver, ovary and testis. Absence of this protein in mice has been seen to be associated with pancreatic β-cell proliferation, hyperinsulinemia and age dependent mild obesity. Previously, we have reported that the absence of WDR13 in diabetic Leprdb/db mice helps in amelioration of fatty liver phenotype along with diabetes and systemic inflammation. This intrigued us to study direct liver injury and hepatic regeneration in Wdr13−/0 mice using hepatotoxin CCl4. In the present study we report slower hepatic regeneration in Wdr13−/0 mice as compared to their wild type littermates after CCl4 administration. Interestingly, during the regeneration phase, hepatic hypertriglyceridemia was observed in Wdr13 −/0 mice. Further analyses revealed an upregulation of PPAR pathway in the liver of CCl4- administered Wdr13 −/0 mice, causing de novo lipogenesis. The slower hepatic regeneration observed in CCl4 administered Wdr13 −/0 mice, may be linked to liver hypertriglyceridemia because of activation of PPAR pathway.