Study on preserved propagation of Polyscias fruticosa (L.) Harms – Araliaceae by direct regeneration from 3-years-old shoot tips. Shoot tips of 1.0 to 1.5cm long taken from field growing Polyscias fruticosa (L.) Harms were sterilized and cultured in Murashige and Skoog (MS) medium supplemented with 1.0mg/l BAP and 0.5mg/l IBA. After 8 weeks, these shoots began to regenerate directly from axil but quantity was not numerous and gave 2-3 new shoots after 4 more weeks. The results of rapid propagation of Polyscias fruticosa (L.) Harms’s shoots after 1, 3 and 4 months culture in MS + 2.0mg/l BAP + 0.5mg/l IBA environment showed that it was the best combination for shoot proliferation. The single shoots gained 2-3cm long were separated from shoot bushes and cultured in MS + 1.0mg/l IBA + 0.5mg/l BAP environment to make root the best. In this environment, shoots began to rooting after 11 days, gained 100% of rooting after 20 days. The plantlets were successfully transferred to PE bags. After 2 weeks, plantlets grew new roots with the rate of live 80-95%
Araliaceae ; Regeneration

The study was carried out to examine the proliferation in vitro of Polyscias fruticosa (L.) Harms. Results: Using HgCl 2 with concentration of 0.1% within 15 minutes and H2O2 within 30 minutes was the best of decontamination time. With the time, the live rate was >80%. Meristems of the branch of Polyscias fruticosa (L.) Harms were induced to develop into plantlets in a medium containing MS-minerals and 2.0 mg/l kinetin. In this medium, an average of 3.4+-0.62 shoots in about 60 days. 100% rooting of the shoots were induced in MS medium supplemented with 1.0 mg/l IBA in 30 days. Rooted planted were grown in the green house.
Araliaceae ; Greenhouse Effect

Both ethanol extracts of the bark and leaves of Schefflera elliptica (Blume) Harms (Araliaceae) showed antistress effect on mice at the oral doses of 158 and 167 mg/kg body weight, respectively. The bark proved to be more effective than the leaves, and their efficacity increased with the doses. Both extracts possessed in vitro antioxidant activity at the concentrations of 50-100 mcg/ml, the bark also being more effective than the leaves. The total saponin extract of the bark exhibited antioxidant effects at the levels of 5-25 mcg/ml. So, saponins might be the chemical constituents determining the antioxidant activity.
Plants ; Biochemistry ; Araliaceae ; Therapeutics

By NAMBA ‘weight method from leaf, root and stem of Schefflera sp. triterpen saponins were qualified. By thin layer chromatography, 11 stains of saponin were determined on leaf, 7 on stem and root with the quantities of 3,66%, 0,93%, 0,77% in absolutely dried leaf,stem and root, respectively. By thin layer chromatography 4 stains of total sapogenins of the leaf and 2 genins were isolated on column chromatography. Fusion points,chromatography,IR spectrum, 1H-NMR,13 C-NMR spectrography,DEPT,HMQC and HMBC had showed oleanolic and hederagenin in the structure of 2 genins
Saponins ; Araliaceae ; Sapogenins

Experimentally, hepatoprotective effect of DinhLang was expressed in mice. The extract of Dinh Lang leaf suppressed the increase of MDA level in brain and in liver of mice suffered from CCl4- induced hepatitis with antioxydant activity stronger than that of mixed extract and extract prepared from the root. Dinh Lang had manifested liver protective effect in tetrachlorurcarbone –induced acute hepatitis. Antioxydant effect expressed in the inhibition against the processes of peroxydation of cell membrane can be one of the mechanism of liver protective effect of Dinh Lang
Antioxidants ; Araliaceae ; liver

Phytochemical constituents were isolated from the fruits of Acanthopanax chiisanensis by repeated column chromatography. Their structures were identified as beta-sitosterol (1), daucosterol (2), sesamin (3), chiisanogenin (4), and 22alpha-hydroxy chiisanogenin (5) by spectroscopic analysis (MS, 1H-, and 13C-NMR). Compounds 1 - 5 were isolated for the first time from the fruits of A. chiisanensis.
Eleutherococcus* ; Araliaceae ; Chromatography ; Fruit* ; Terpenes*

Extracts from roots and leaves of Polyscias fruticosa Harms. (Araliaceae) and their combination have been studied on lipid peroxidation in mouse brain using an auto-oxidation and a free radical generating system: the Fenton’s reagent (ferrous iron + hydrogen peroxide). All the root, leaf and combined extracts have inhibitory action on peroxidation of lipids and trolox, a water-soluble analogue of vitamin E used as a reference antioxidant compound. The results also indicate that the leaf extract is the strongest of all.
Biochemistry ; Plants ; Plants, Medicinal ; Araliaceae

OBJECTIVETo study active constituents of Macropanax rosthornii in treating rheumatoid arthritis.

METHODSilica gel column chromatography, preparative HPLC and modern spectrum techniques were applied for a systematic study on chemical constituents contained in M. rosthornii.

RESULTTwelve compounds were separated from M. rosthornii and identified as serratagenic acid (1), serjanic acid (2), betulinic acid (3), 6beta-hydroxy-3-oxo-olean-12-en-28-oic acid (4), 3-O-alpha-L-arabinopyranosyl serratagenic acid (5), 3-O-alpha-L-arabinopyranosyl serratagenic acid-29-methyl ester (6) , 3-O-alpha-L-arabinopyranosyl serratagenic acid-28-O-beta-D-glucopyranosyl ester (7), scopoletin (8), beta-sitosterol (9), daucosterol (10), 3,4-dihydroxybenzoic acid (11), and stearic acid (12).

CONCLUSIONAbove compounds are separated from M. rosthornii for the first time.

Eleven compounds were isolated and purified from the barks extract of Nothopanax delavayi and their structures were identified as serratagenic acid-3-O-alpha-L-arabinopyranosyl-28-O-beta-D-glucopyranosyl ester (1), serratagenic acid-3-0-alpha-L-arabi-nopyranosyl-28-O-[alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester (2), serratagenic acid (3), serratagenic acid-3-O-alpha-L-arabinopyranoside (4), serratagenic acid-beta-O-beta-(2', 4'-O-diacetyl) -D-xylopyranosyl-28-O-[alpha-L-rhamnopy-ranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->46)-beta-D-glucopyranosyl] ester (5), serratagenic acid-3-O-alpha-(4'-O-acetyl)-L-arabino pyrano-syl-28-0- [-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester(6), serratagenic acid-3-O-alpha-(2'-O-acetyl)-L-arabinopyranosyl-28-O-[-alpha-L-rhamnopyranosyl- (1-->4) -beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester(7), serratagenic acid-3-0-beta-D-xylopyranosyl-28-O-[-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester (8), protocatechuic acid (9), ethyl caffeate (10) and caffeic anhydride (11) by physicochemical properties and spectroscopic data analysis. Among them, compounds 3-4 and 9-11 were firstly isolated from the genus Nothopanax, and compounds 5-8 were isolated from this plant for the first time.
Araliaceae ; chemistry ; Drugs, Chinese Herbal ; chemistry ; Magnetic Resonance Spectroscopy ; Molecular Structure ; Plant Bark ; chemistry

Nineteen compounds, including one organic acid (1), one anthraquinone (2), one amide (3), and sixteen triterpenoid saponins (4 - 19) were isolated from the leaves of Acanthopanax henryi (Oliv.) Harms (Araliaceae). Their structures were determined on the basis of physicochemical properties and spectral analyses (HR-MS and NMR). Among them, compounds 2, 3, 7, 12 and 19 were new within Araliaceae. Compounds 4, 5, 9 - 11, 13, 14, 16 and 18 were reported for the first time from the Acanthopanax genus. Except for compounds 4 and 9, other compounds were isolated from A. henryi (Oliv.) Harms for the first time. The rare anthraquinone, compound 2, significantly decreased the production of NO and the levels of other inflammatory factors, such as TNF-alpha and IL-6, in lipopolysaccharide (LPS)-stimulated macrophages in a dose-dependent manner. This is the first time to report anti-inflammatory effect of this compound.
Eleutherococcus* ; Araliaceae ; Interleukin-6 ; Macrophages ; Nitric Oxide ; Saponins ; Tumor Necrosis Factor-alpha