1.Overexpression of vascular endothelial growth factor in epidermis of human skin ulcerous margin
Ling SUN ; Ye ZHAO ; Aoxue WANG
Chinese Journal of Medical Aesthetics and Cosmetology 2001;0(03):-
Objective To investigate the expression of vascular endothelial growth factor(VEGF) in human skin ulcer margin. Methods Ulcer biopsy samples were stained with anti VEGF and CD34 antibodies by immunohistochemistry .Results VEGF was not expressed in normal epidermis. The ulcer specimen was divided into normal,peripheral,marginal and central parts. Epidermis of normal part was not stained. VEGF was obviously expressed in peripheral upper epidermis of ulcer and lower epidermis of ulcer margin. Central part was not stained due to shedding of epidermis. And also CD34 staining demonstrated that dermal capillaries increased obviously and lumen dilated, which was marked in the periphery and the margin of ulcer. Conclusions VEGF is secreted in skin ulcerous epidermis and promotes angiogenesis and vascular dilataion which play a role in ulcer healing.
2.Development and validation for the determination of total flavonoids and luteolin from Pteris multifida Poir
Mengwei LI ; Yuemei MA ; Aoxue LIU ; Hao WANG ; Xinjing CHEN ; Yang LIU
International Journal of Traditional Chinese Medicine 2017;39(7):617-621
Objective To determine the content of total flavonoids and luteolin from Pteris multifida Poir. Methods The content of total flavonoids was determined by gradient elution of macroporous resin D101 and ultraviolet spectrophotometry. The content of luteolin was determined by HPLC. The analysis was performed on a RP-C18 column(4.6 mm×250 mm, 5 μm) with aceconitrile-0.2% phosphoric acid (35:65) as the mobile phase at a flow rate of 1.0 ml/min, and 30 ℃ temperature. Results The detection of wave length was set at 349 nm. The content of luteolin was 0.015%, 0.019%, 0.016%, and the content of total flavonoids was 0.015%, 0.019%, 0.016%, respectively. Conclusions The method is suitable for the determination of flavonoids componets from Pteris multifida Poir.
3.Research advances of cardiac graftvasculopathy
Ruiqi SANG ; Aoxue WANG ; Yihan TANG ; Zhiyong WU ; Yongle RUAN
Chinese Journal of Organ Transplantation 2023;44(11):698-702
From the perspectives of the pathogenesis, diagnosis and treatment of cardiac graft vasculopathy(CGV), this review summarized the current understanding and cognition of its pathology, monitoring, diagnosis and treatment to provide rationales for better survivals of CGV.
4.Recent advance in pathogenesis and treatments of neurological complications related to patent foramen ovale
Aoxue WANG ; Yihan TANG ; Ruiqi SANG ; Zhiyong WU ; Yongle RUAN
Chinese Journal of Neuromedicine 2023;22(12):1282-1286
Patent foramen ovale (PFO) is the most prevalent congenital heart disease, often accompanied by neurological symptoms as migraine, unexplained dizziness, and even anxiety and depression. Recent research findings indicate that the pathogenesis of neurological complications related to PFO involves abnormal embolism hypothesis, vasoactive substance hypothesis, impaired cerebral blood flow regulation and genetic inheritance. Treatments include primarily encompass pharmacological intervention and foramen ovale occlusion. This article summarizes the aforementioned research progress in order to provide clinical guidance for managing nervous system complications related to PFO.
5.Clinical and genetic analysis of a patient with Loeys-Dietz syndrome due to variant of TGFBR2 gene.
Yueli WANG ; Zhihua KONG ; Long WAN ; Aoxue WANG ; Xiaoyan LI
Chinese Journal of Medical Genetics 2023;40(12):1531-1535
OBJECTIVE:
To explore the genetic basis of a patient with clinically suspected Loeys-Dietz syndrome (LDS).
METHODS:
A child who had presented at Beijing Anzhen Hospital in September 2018 was selected as the study subject. Clinical data and family history of the patient were collected, along with peripheral blood samples of the proband and his parents. Whole exome sequencing (WES) was carried out through next-generation sequencing.
RESULTS:
Candidate variants were searched through bioinformatic analysis focusing on genes associated with hereditary aortic aneurysms. Candidate variant was verified by Sanger sequencing. The patient was found to have cardiovascular abnormalities including early-onset aortic dilatation and coarctation, and LDS syndrome was suspected. WES revealed that he has harbored a heterozygous c.1526G>T missense variant of the TGFBR2 gene. The same variant was not found in either parent and was predicted as likely pathogenic (PM1+PM2_Supporting+ PM6+PP3+PP4) based on the guidelines from the American College for Medical Genetics and Genomics (ACMG).
CONCLUSION
The TGFBR2 c.1526G>T variant probably underlay the LDS in this patient and was unreported previously in China. Above finding has enriched the mutational spectrum of the TGFBR2 gene associated with the LDS and provided a basis for the genetic counseling for the patient.
Child
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Humans
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Male
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China
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Computational Biology
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Family
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Loeys-Dietz Syndrome/genetics*
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Mutation
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Receptor, Transforming Growth Factor-beta Type II/genetics*
7.Development of small molecule extracellular signal-regulated kinases (ERKs) inhibitors for cancer therapy.
Xiaoli PAN ; Junping PEI ; Aoxue WANG ; Wen SHUAI ; Lu FENG ; Faqian BU ; Yumeng ZHU ; Lan ZHANG ; Guan WANG ; Liang OUYANG
Acta Pharmaceutica Sinica B 2022;12(5):2171-2192
The mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway is widely activated by a variety of extracellular stimuli, and its dysregulation is associated with the proliferation, invasion, and migration of cancer cells. ERK1/2 is located at the distal end of this pathway and rarely undergoes mutations, making it an attractive target for anticancer drug development. Currently, an increasing number of ERK1/2 inhibitors have been designed and synthesized for antitumor therapy, among which representative compounds have entered clinical trials. When ERK1/2 signal transduction is eliminated, ERK5 may provide a bypass route to rescue proliferation, and weaken the potency of ERK1/2 inhibitors. Therefore, drug research targeting ERK5 or based on the compensatory mechanism of ERK5 for ERK1/2 opens up a new way for oncotherapy. This review provides an overview of the physiological and biological functions of ERKs, focuses on the structure-activity relationships of small molecule inhibitors targeting ERKs, with a view to providing guidance for future drug design and optimization, and discusses the potential therapeutic strategies to overcome drug resistance.