Objective To establish a new rat model of biliary atresia by pure ethanol injection into the common bile duct.Methods A catheter was inserted and fixed in the common bile duct in male SD rats .Saline (8 rats) or pure ethanol (16 rats) was injected through the catheter ,respectively, and the biochemical and pathological changes in the rats were examined .Results SD rats in the experimental group were divided into a persistent injury and a restoration of liver dysfunction groups according to pathological and biochemical detection .In the persistent injury group , biochemical impair-ments were significantly higher at 8 weeks after ethanol injection than those in the control group and restoration group .Dis-tinct pathological changes in the liver were observed using HE , SMA, and Masson staining .Conclusions It is a reliable animal model of biliary atresia induced by injection of pure ethanol into the common bile duct in the rat .It will provide a useful tool in future studies of biliary atresia .

Objective To investigate the short-and mid-term prognosis and its influencing factors of Kasai portoenterostomy for type Ⅲ biliary atresia.Methods 220 infants with type Ⅲ biliary atresia who underwent Kasai surgery at Capital Pediatric Institution from Jan 2014 to June 2017 were retrospectively reviewed.There were 88 males and 132 females,with an average age at operation (60 ±23) d.The followup time ranged from 12 to 53 months.Results The survival rate with native liver (SNL) at 1,2,and 3 years after Kasai operation were 65％,59.6％,and 57.0％,respectively.The clearance rate of jaundice within 3 months after Kasai was 50.5％;3-year SNL of infants whose jaundice disappeared or did not after operation were 86.8％ and 27.1％ (P ＜ 0.05).The overall incidence of cholangitis was 60％.The clearance rate of jaundice in children with or without cholangitis was 55.3％ and 43.2％,while the 3-year SNL were 62.4％ and 49.6％,respectively (P ＞ 0.05).Conclusion The 3-year survival rate on their native liver after Kasai operation in infants with type Ⅲ biliary atresia can reach up to 57％.The clearance of jaundice within 3 months post-operation is the main factor affecting prognosis.The age at surgery and the occurrence of cholangitis do not affect the survival rate of native liver in the short-and mid-term prognosis.