1.Giant Myofibroblastoma of the Male Breast: A Case Report and Literature Review
Kamal Kataria ; Anurag Srivastava ; Lavleen Singh ; Vaishali Suri ; Rajni Yadav
Malaysian Journal of Medical Sciences 2012;19(3):74-76
Myofibroblastomas are soft-tissue neoplasms that are thought to arise from myofibroblasts. They are mostly observed in males 41–85 years of age; however, this lesion also occurs in women. The usual clinical presentation is a unilateral painless lump that is not adherent to overlying or underlying structures. Microscopically, myofibroblastomas can be divided into 5 subtypes: classical, epithelioid, collagenised, cellular, and infiltrative. Mammary ducts and lobules are absent in the typical histological subtypes and the adjacent breast parenchyma may form a pseudocapsule. The majority of myofibroblastomas are immunoreactive for CD34, desmin, smooth muscle actin, and vimentin and are negative for cytokeratin and S-100 protein. We present a case of a giant myofibroblastoma arising in the background of gynecomastia in an adult male.
2.Myeloid sarcoma of the breast in an aleukemic patient:a rare entity in an uncommon location
Aasma Nalwa ; Devajit Nath ; Vaishali Suri ; Mohamed Amjad Jamaluddin ; Anurag Srivastava
The Malaysian Journal of Pathology 2015;37(1):63-66
Myeloid sarcoma (MS) is an extramedullary solid neoplasm of immature myeloid cells. These tumours
usually develop in concurrence with or following acute leukemia. The breast is an uncommon site
for presentation of this tumour, where it is often misdiagnosed as lymphoma or carcinoma.A 33-
year-old female presented with a right breast lump in a private hospital, which was diagnosed as
ductal carcinoma on lumpectomy. Subsequently she developed a lump in the left breast and a similar
diagnosis of carcinoma was made on biopsy. A left mastectomy was performed. Histopathological
examination revealed a tumour composed of mononuclear cells arranged in sheets and cords with
round to oval vesicular nuclei and occasional prominent nucleoli. IHC for CK was very weak and
focal. The tumour cells were immunonegative for ER, PR, Her2neu,epithelial membrane antigen,
e-cadherin, CD3 and CD20. Diffuse immunopositivity for myeloperoxidase, CD34 and CD117
established a diagnosis of myeloid sarcoma. A histopathological review of the right breast lesion,
with immunohistochemistry, also confirmed the diagnosis of myeloid sarcoma. Investigatory workup
for acute myeloid leukemia, including bone marrow aspirate and biopsy and karyotypic studies,
proved negative. The patient was treated with high dose cytarabine (HDAC) regimen and was disease
free during the 12-month follow-up.Although extremely rare, awareness of such a presentation is
crucial. This case also illustrates that careful histopathological review along with an expanded panel
of immunohistochemistry is extremely important for recognizing such cases as a misdiagnosis can
lead to unnecessary surgery and inappropriate therapy.
3.Evaluation of inhibitory effect of the plant Phyllanthus amarus against dermatophytic fungi Microsporum gypseum.
Anurag AGRAWAL ; Shalini SRIVASTAVA ; J N SRIVASTAVA ; M M SRIVASAVA
Biomedical and Environmental Sciences 2004;17(3):359-365
OBJECTIVEThe antifungal activity of various solvent extracts (such as ether, chloroform, ethyl acetate and ethyl alcohol) of the plant Phyllanthus amarus against dermatophytic fungi Microsporum gypseum was observed.
METHODAntifungal bioassay in terms of reduction in weight, colony diameter and sporulation of the target fungal colony was carried out using Broth Dilution method. Results Root part of the plant, extracted in various organic solvents did not show any noticeable antifungal activity. The percentage inhibition observed in different solvent extracts of aerial part was found as reduction in weight: chloroform [50.3%], ethyl acetate [27.7%] and ethyl alcohol [12.1%], reduction in colony diameter: chloroform [53.4%], ethyl acetate [31.4%] and ethyl alcohol [15.0%] and reduction in sporulation: maximum inhibition in chloroform extract, at test concentration of 4000 ppm at incubation period of 8 days.
CONCLUSIONChloroform fraction of the aerial part of the plant P. amarus shows significant inhibitory effect against dermatophytic fungi M. gypseum and requires chemical characterization for its bioactive principle.
Acetates ; chemistry ; Antifungal Agents ; pharmacology ; Chloroform ; chemistry ; Ethanol ; chemistry ; Microsporum ; drug effects ; growth & development ; Phyllanthus ; Plant Components, Aerial ; Plant Extracts ; pharmacology ; Plant Roots
4.Comparison of Modified Rose Method of Thyroid Palpation versus other Methods for the Detection of Retrosternal and Nodular Goitre
Santosha Kumar Pattashanee ; Gopal Puri ; Kamal Kataria ; Piyush Ranjan ; Anita Dhar ; Anurag Srivastava ; Surabhi Vyas ; Yashdeep Gupta ; RM Pandey
Journal of the ASEAN Federation of Endocrine Societies 2022;37(1):4-13
Objectives:
This is a diagnostic test research study to evaluate the various existing methods of thyroid examination and their comparison with the novel modified Rose method. It also aims to measure inter-examiner variation in clinical findings based on the level of education and training, as compared to ultrasonography.
Methodology:
This cross-sectional study was conducted at a tertiary care hospital with 83 patients presenting to surgery OPD with neck swelling. Each patient was examined by one trained Junior Resident and a Surgery Consultant with all the four methods and with ultrasonography. Data was analysed by Stata-14, agreement between the two categorical variables was assessed by Kappa. In case of continuous variable agreement was assessed by Intra class correlation and Bland-Altman plot.
Results:
Modified Rose method by the consultant has the highest sensitivity (98%) and diagnostic odds (210) as compared to others but its specificity ranges from 46.7-91.1% to diagnose retro-sternal extension of a goiter. It has 93.98% agreement for identification of nodules. It has a high specificity (Consultant - 100%, Resident - 95.5%) with relatively lower sensitivity (Consultant - 94%, Resident - 86.8%) to diagnose solitary thyroid nodule (STN) but the sensitivity and specificity for diagnosing a multinodular goitre (MNG) was high. However, the highest sensitivity to diagnose STN was highest with Crile’s method, but specificity was low. Lahey’s method was a better clinical method to palpate lymph nodes compared to the other three.
Conclusion
Examination in modified Rose’s position is a better method of clinical examination of thyroid especially in patients with occult substernal extension. Lahey’s method is a better method to examine cervical lymph nodes.