4 lots of DPT mixte vaccine were produced with whooping-cough vaccine concentrated fluid by fermentor D300. The product was reached WHO and Hanoi National Controlled Centre criterion of safety and efficacy
Antigens, Bacterial ; Pertussis Vaccine

A study was conducted to test the sterility of 416 final lots of OPV produced by POLIOVAC, Vietnam from 1994 to 2003 in thioglycolate and soybean casein digest media under the standard operating procedure. The study results showed that all the tested lots (461/461 = 100%) had met the requirements for sterility
Infertility ; Poliomyelitis ; Antigens, Bacterial

The entry of gastric carcinoma cells ASG via the interaction of the vesicles of Helicobacter pylori VNH-85 strain was studied. The results showed that: The vesicles of Helicobacter pylori contained many protein toxins such formed from the outer membrane of bacterium H. pylori. The entry of them into gastric carcinoma cells ASG C120 led to increased synthesis of interleukin IL-8. So that, protein CagA could enter into gastric epithelium cells via interaction of bacteria, into gastric epithelium cells, protein CagA increased synthesis of interleukin IL-8 over 8 times.
Helicobacter pylori ; Cells ; Antigens, Bacterial ; Bacterial Proteins

Study was performed on 100 serum samples of patients (15-70 aged) treated of gastro-duodenal diseases and 31 healthy persons by Immunoblot assay. The results showed that: the prevalence of H.pylori positive in patients with gastric ulcer, duodenal ulcer and gastritis was 76.5, 95.8 and 73%, respectively. The prevalence of H. pylori seropositive was found 64.5% in healthy persons. The prevalence of anti-CagA and anti-VacA antibodies was 86.2% and 60.6% (respectively) in patients and was 70% and 40% (respectively) in healthy person.
Antigens, Bacterial ; Bacterial Proteins ; Helicobacter pylori ; Antibodies ;

The first 5 lots of Japanese encephalitis vaccines produced from Beijing-1 strain: JB011203, JB021203, JB030104, JB040204 and JB050304 were controlled of quality on chemical components and virus morphology in vaccine, using reference vaccine EJP034A (BIKEN-Japan). The results showed that: TCA-protein was 13.2- 14.5 µg/ml, the reference was 11.1 µg/ml (WHO standard 5-40 µg/ml). The thimerosal content was 82-84 µg/ml, the reference 76,3 µg/ml (WHO standard ≤120 µg/ml). The formaldehyde content was 0.061-0.063 µg/ml, the reference was 2.19 µg/ml (WHO standard ≤100 µg/ml). pH was 7.02-7.04, the reference was 7.10 (WHO stand. 6.8-7.4). The viruses morphology after negative stain were observed on electromicroscope JEM1010 with enlarge x 90.000 and x 150.000. The identical ball form of purified viruses with the intact surface antigen. 5/5 lots were passed the minimum requirements of biological products of WHO.
Japanese Encephalitis Vaccines ; Antigens ; Bacterial

Whole studied poliomyelitis vaccine bulk lots had had sensitivity in high temperature and low concentration of NaHCO3. They all met WHO standards
Poliomyelitis ; Antigens, Bacterial ; Reference Standards

The enterobacterial common antigen (ECA) is a polysaccharide composed of polysaccharide repeats that are located in the outer membrane of almost all Enterobacteriaceae bacteria and has diverse biological functions. ECA is synthesized by the synergistic action of multiple genes that are present in clusters on the genome of Enterobacteriaceae bacteria, forming the ECA antigen gene cluster, an important virulence factor that plays a role in host invasion and survival of Enterobacteriaceae in vivo. ECA also plays an important role in the maintenance of the bacterial outer membrane permeability barrier, flagella gene expression, swarming motility, and bile salts resistance. In addition, ECALPS, anchored in the core region of bacterial lipopolysaccharide, is an important surface antigen for bacteria, stimulating high levels of antibody production in the host and could be a target for vaccine research. This review summarizes ECA purification, genes involved in ECA biosynthesis, its immunological characteristics, biological functions and clinical applications.
Antigens, Bacterial/genetics* ; Enterobacteriaceae/genetics* ; Lipopolysaccharides ; Polysaccharides

Recombinant bacterial vector vaccines have been widely used as carriers for the delivery of protective antigens and nucleic acid vaccines to prevent certain infectious diseases because of their ability to induce mucosal immunity, humoral immunity and cellular immunity. However, protective antigens and nucleic acids recombined into bacterial vector vaccines are difficult to be released into host cells because of the presence of bacterial cell wall. Vaccine strains that are residual in animals or livestock products may also cause environmental contamination and spread of the vaccine strains. The effective solution for these problems is to construct an auto-lysis system that can regulate the vaccine strains to grow normally in vitro while lysis in vivo. The lysis systems that have been applied in germs mainly include: the lysis system based on regulated delayed peptidoglycan synthesis, the lysis system based on the regulation of bacteriophage lysis protein and the lysis system based on the toxin-antitoxin system. In addition, a potential lysis system based on bacterial Type Ⅵ Secretion System (T6SS) is also expected to be a new method for the construction of auto-lysis strains. This review will focus on the regulatory mechanisms of these bacterial lysis systems.
Animals ; Antigens, Bacterial ; Bacterial Vaccines ; Vaccines, Attenuated ; Vaccines, DNA

The amount of lymphocyte B and the responding capacity to create antibody of activated lymphocyte B are two important parameters to assess the humoral-immunity responding capacity of healthy and unhealthy bodies. The amount of lymphocyte B were determined in 22 healthy Katu minority living in Nam Dong district, Hue city with technique direct immuno-fluorescence, and 64 Katu people have HbsAg(-) and anti-HbsAg(-), who were studied the responsive capacity to create antibody against hepatitis B vaccine with technique ELISA. The control group are Kinh people of the same criterion. Results: The amount of lymphocyte B in 1mm3 blood: 354.9  101.1; lower than that of the Kinh people of the same age, same occupation, with p < 0.05. The responding capacity to create antibody against hepatitis B vaccine at good standard, 84.4%; that is higher than that of the Kinh people, however with p > 0.05
Lymphocyte Count ; hepatitis B ; Antigens, Bacterial ; blood ; Antibody Formation

OBJECTIVE7 variable but nonculturable-state strains of Enterotoxigenic Escherichia coli (ETEC) during the routine bacterial subculture were found in our lab and their morphology and antigen studied. Biological features, antigens and pathogenicity of the revertants were also tested and compared to that of the initial strains in order to detect their variations.

METHODSBiological variations between the variable but nonculturable-state and the revertant of every strain were detected, using the routine gram-staining, reverting the isolates in animal intestinal, reverting their pathogenicity, serological agglutination, biochemical identifications and antibiotic resistance tests.

RESULTSFor the 7 variable but nonculturable-state strains of ETEC,other than the trains that had changed into sphero vegetale cells, there were no other obvious variations found. However, high pathogenicity of these strains still remained.

CONCLUSIONThe presence of variable but nonculturable-state strains suggested that the routine method of bacteria storage should be changed and more attention should be paid to realize the existence of this kind of bacteria during the routine surveillance of the communicable diseases.