AIM:To investigate the antiemetic effect of preadministration of Banxia Fuling Capsule(Rhizoma Zingiberis recens,Rhizoma Pinelliae,Poria,etc)(BFC)in pigeons and dogs,and to explore the relationship between antiemetic effect of BFC and 5-hydroxytryptamine(5-HT).METHODS:The pigeons and dogs were pretreated with BFC for 3 days.Acute emesis of pigeons and dogs induced by intravenously injecting cisplatin were observed.The concentraction of 5-HT in the serum,small intestine and brainstem of the animals were determined by spectrofluorimetry.RESULTS:The number and incidence of the emesis decreased in the treatment of BFC in dogs and pigeons;The levels of 5-HT in brainstem and small intestine decreased in pigeons,and so was it in the serum of dogs.CONCLUSION:BFC may inhibit emesis induced by cisplatin in dogs and pigeons,the antiemetic effect of BFC may decrease the synthesis and release of 5-HT in brainstem and small intestine.