The great majority of human diseases are directly or i nd irectly associated with genes. Gene mapping and genetic analysis for human compl ex polygenic disorders has become a hot-spot and the neck of the bottle in the medical genetics research recently. The approach to genome-wide search has pla yed an important role in the respect.

Objective To identify the gene mutations and mu tating patterns in a pedigree with X-linked anhidrotic ectodermal dysplasia(EDA)so as to provide a basis for gene diagn osis and genetic counselling of this disorder.Methods Polymerase chain reaction-single s trand conformation polymorphism(PCR-SSCP)analysis and DNA sequencing of amplified prod ucts were performed to screen mutati ons and mutating patterns of EDA1gen e,responsible for EDA pathogenesis,i n a X-linked EDA family of Han people.Results Abnormal single strand bands were found in the amplified fra gments as well of exon 1of EDAgene in t he patients as well as their mothers,the carriers.The DNAsequencing of ampl ified products revealed a point muta tion at nucleotide 404(C→Gtransversion)in the proband compared with that of t he normal controls,which resulted i n the transversion of histidine with glutamine at codon 54in the ectodysplasin-A(H54Q).Meanwhile there were heterozyous double peaks of nucleotide Cand Gat the same position in his mother.Conclusion A missense mutation(404C→G)in exon 1of EDA1gene has been determined in the pedigree with X-linked EDA,which is probably one of the molecular bases of EDA pathogenesis.

8 patients, 6 males and 2 females, aged 26-56 years, with delayed neurologic sequelae following CO intoxication, were treated. The lucid intervals before the appearance of neurological sequelae varied from 5 days to 3 months, and the course of disease lasted for 7 days to 2.3 years. The clinical manifestations were mainly dementia, mutism, urinary or fecal incontinence, and gait disturbance. Among the 8 patients, clinical cure was obtained in 5 and improved in 3 after intrathecal oxygen administration.

Total glucosides of paeony (TGP, 50 mg/kg 7d, ig) could enhance the episode duration of slow-wave sleep (SWS) in normal rats, and restore the sleep parameters in the insomniac rats induced by caffeine ( 1 2. 5 mg/kg 7 d,ip)nearly to the normal level. It (50 mg/kg 3 d,ig) also increased significantly thetotal time of SWS and paradoxical sleep (PS) in the swimming rats (water temperature 25?1℃, swimming time 30 min). These results suggest that TGP probably improve the sleep of rats under the different states.

The present valuation of experimental teaching effect can not adapt to the needs of the experimental reform and develpoment due to its deficiency. After setting up the experimental curriculum,A Multivariate method has been developed to evaluate students’ experimental ability from their innovating,operating,expressing ability and scientific quality.

Objective To explore HLA-DQA1 and DQB1 alleles in their association with genetic susceptibility to psoriasis vulgaris(PV) in Han Chinese. Method Polymerase chain reaction sequence specific primers (PCR-SSP) method was used to analyze the frequencies of HLA-DQA1 and DQB1 alleles among 189 patients with PV and 273 healthy controls. Results ①HLA-DQA1*0104 and DQA1*0201 alleles were positively associated with PV (Pc

Objective To investigate the effect of ajoene on thrombosis and explore the possible mechanism.Method SD rats were randomly divided into five different groups and administered ajoene in 7d.The effect of ajoene on thrombosis in rats was observed with the thrombogenesis model of artery-vein bypass.The serum level of vascular cell adhesion molecule-1(VCAM-1) and P-selectin(Ps)were measured.To observe the effect of ajoene on platelet aggregation,arachidonic acid was used to induce platelet aggregation and platelet function was evaluated by turbidimetry in rabbit.Results Ajoene significantly inhibited the the thrombosis of artery-vein bypass in rats and reduced rate of platelet maximal aggregation and decreased the P-selectin concentration in serum at 25,50,75mg/kg (P

Research-Based Teaching is to cultivate students’ competence of study and innovation,which requires teachers to change their concept and make careful design and full preparation before they put it into practice. The practice shows that adopting the teaching method of bringing students’ autonomous study into full play and intensifying the training of their scientific research is an important approach to implement it. Research-Based Teaching will play a more and more important role in cultivating students’ comprehensive competence.

BACKGROUND:Previous studies have shown that continuous administration of 1×107 of bone marrow mesenchymal stem cel s can induce immune tolerance in rats undergoing kidney transplantation, but it is not yet found clinical y that curcumin exerts an on immunomodulatory effect on kidney transplantation.
OBJECTIVE:To investigate the effects of different concentrations of curcumin on bone marrow mesenchymal stem cel s-induced immune tolerance in rats after kidney transplantation.
METHODS:Rat model of kidney transplantation was made, and rat models were randomly divided into four groups:transplantation group with no treatment;bone marrow mesenchymal stem cel s group (cel group) injected with 1×107/kg bone marrow mesenchymal stem cel s via the left iliac vein (before peritoneal suture) and tail vein (from the 2nd day) for 10 days;bone marrow mesenchymal stem cel s+low/high dosage of curcumin groups (low/high dosage curcumin groups) injected intragastrical y with 2 or 10 mg/kg curcumin combined with injection of bone marrow mesenchymal stem cel s for 10 days. Transforming growth factor-β1 protein expression in the kidney tissues was examined by immunohistochemical staining. The concentrations of interleukin-2 and interleukin-6 in serum were detected by ELISA assay.
RESULTS AND CONCLUSION:After kidney transplantation, the protein expression of transforming growth factor-β1 in renal tubular epithelial cel s and renal interstitial cel s as wel as the concentrations of interleukin-2and interleukin-6 in serum were significantly higher in the transplantation group than the other groups (P<0.05). Compared with the cel transplantation group, the protein expression of transforming growth factor-β1 as wel as the concentrations of interleukin-2 and interleukin-6 reduced significantly in the low/high dosage curcumin groups (P<0.05). These findings indicate that simultaneous administration of curcumin and bone marrow mesenchymal stem cel s can effectively inhibit immune rejection reaction and improve renal function in rats after kidney transplantation.