1.Mechanism of SIRT1 acting on tumor formation
Anning FENG ; Xiangshan FAN ; Qin HUANG ; Lihua ZHANG
Journal of Medical Postgraduates 2003;0(07):-
SIRT1 is an NAD+-dependent histone deacetylase,involved in many physiological processes,such as cell aging,apoptosis and differentiation.Recent studies have shown that SIRT1 plays a significant role in tumor formation and progression.It deacetylates a wide range of histone and non-histone substrates and regulates gene expression and protein activity that are associated with neoplastic apoptosis and proliferation.
2.Palliative surgical treatment and minimally invasive biliary drainage on hilar cholangiocarcinoma effect analysis
Anning XIA ; Shouxiang ZHANG ; Yong DENG ; Yujie FENG ; Bingyuan ZHANG
International Journal of Surgery 2015;42(11):748-751,封4
Objective To investigate the effect of palliative surgical treatment and minimally invasive biliary drainage of hilar cholangiocarcinoma.Methods Retrospectively collected 244 hilar cholangiocarcinoma patients clinical data in Affiliated Hospital of Qiingdao University between Jan.1,2008 to Dec.31,2011.Survival accoding to different treatment methods was compared using Kaplan-Meier method.The continuous measurement data were analyzed using the one-way ANOVA and the U test.The categorical variable were analyzed using the chi-square test or Fisher exact test.Result Among 244 patients, the R1/R2 resection group in 93 patients, the endoscopic retrograde biviar drainage group in 69 patients, the percutaneous transhepatic cholangial drainage group in 82 patiens.Three groups of median survival time and l-, 2-, 3-year survival rate and median survival time were (13.5 months, 8.9 months, 8.6 months), (63.0%, 24.7%, 7.4%), (33.3%, 3.3%, 0), (32.4%,4.2%, 0), respectively.There was significant difference in the survival time between R1/R2-resections and endoscopic retrograde biviary drainage treatments (P < 0.001).R1/R2-resections and percutaneous transhepatic cholangial drainage treatments were statistical differences in survival time (P < 0.001).Endoscopic retrograde biviary drainage and percutaneous transhepatic cholangial drainage treatments were no statistical differences in survival time (P =0.971).Conclusions Palliative surgical treatment for hilar cholangiocarcinoma patients had a more significant effect.Endoscopic retrograde biviary drainage and percutaneous transhepatic cholangial drainage of minimally invasive jaundice reducing method for prognosis in patients with hilar cholangiocarcinoma was same.
3.Comparative analysis of clinical short-term outcomes of Da Vinci robot-assisted spleen-preserving distal pancreatectomy and laparoscopic spleen-preserving distal pancreatectomy
Yong DENG ; Anning XIA ; Shouxiang ZHANG ; Yujie FENG ; Bingyuan ZHANG
International Journal of Surgery 2015;42(9):596-599,封3
Objective To compare the clinical result of Da Vinci robot-assisted distal pancreatectomy(RDP) and laparoscopic distal pancreatectomy(LDP), and to evaluate the clinical application experience of Da Vinci robotassisted spleen-preserving distal pancreatectomy.Methods From March 2013 to June 2015, totally 12 patients undergone RDP and 22 patients undergone LDP in our department were analyzed retrospectively.Results Intraoperative blood loss, hospitalization duration and postoperative fast time in RDP group was less than that in LDP group, the spleen-preserving rate and hospitalization expenses were higher in RDP group(P < 0.05).There was no statistically significant difference in the rate of surgery duration and incidence of postoperative complication between two groups (P > 0.05).The following-up period was 1-28 months with a mean of (9.94 ± 8.99) months, 1 case of peritoneal infection occurred in RDP group, 1 case of survival with tumor recurrence and 3 case suffered peritoneal infection in LDP group.Others were no metastasis, recurrence or death.Conclusions RDP is safe and feasible, the short-term prognosis is better than that of LDP.It has advantages of cleat 3 D visual field, stability in control,less invasive,and quick recovery.It is worth further clinical use.
4.MR features of mice spleen lymphocytes labeled with super-paramagnetic iron oxide particles
Anning LI ; Zhenwei YAO ; Xiaoyuan FENG ; Ying MAO ; Wei HUA ; Feng TANG ; Wei LI ; Feifei LUO
Chinese Journal of Radiology 2012;46(3):260-263
Objective To investigate the feasibility of labeling mice spleen lymphocytes with superparamagnetic iron oxide(SPIO)and in vitro MR imaging of the labeled cells.Methods Spleen lymphocytes of 5 mice were isolated and then labeled with SPIO of 100,50,25,15,10,5 μg/ml,which was previously prepared with PLL.Prussian blue staining was performed to show the intracellular iron.Cell viability was compared among fresh,labeled and unlabeled cells.Different concentrations of mice spleen lymphocytes were screened using 3.0 T MR on T2WI,T2 * WI and SWI sequences in vitro.Cell viability was compared using independent-sample t test between groups.The MRI values among different groups were compared using one-way ANOVA.Results SPIO prepared with PLL could successfully label mice spleen lymphocytes,the optimum concentration of SPIO was 5 μg/ml.The Prussian blue staining showed intracellular blue spots and a labeling efficiency of(93.6 ± 2.1)%.Three groups of fresh,labeled and unlabeled cells showed a Trypan blue staining result of(94.8 ± 3.1)%,(88.7 ± 2.7)%,and(88.9 ±3.2)%,respectively; no statistically significant difference was found in cell viability between labeled and unlabeled lymphocytes(t =0.281,P > 0.05); however,the cell viability of fresh cells were statistically significant higher than the labeled and unlabeled lymphocytes(t =8.125 and 7.253 respectively,P <0.05for all).Among the T2 WI,T2 * WI and SWI sequences under the same concentrations of cells,the SWI sequence was the most sensitive.Conclusions The mice spleen lymphocytes can be effectively labeled with SPIO with no impact on cell viability,and MR can be used to track these labeled cells in vitro.The SWI sequence is the most sensitive.
5. Effect of cognitive-behavioral therapy combined with family rehabilitation therapy on improving depression patients
Xuehong CHEN ; Yan FENG ; Haiyan LI
Chinese Journal of Practical Nursing 2019;35(35):2725-2730
Objective:
To explore the effect of cognitive-behavioral therapy combined with family rehabilitation therapy on improving depression patients.
Methods:
Totally 82 depressive patients admitted to our hospital from February 2016 to January 2017 were randomly divided into the control group and the observation group according to the random number table method, with 41 cases in each group. Patients in the control group were treated with family rehabilitation therapy, while the patients in the observation group were treated with cognitive behavioral therapy on the basis of family rehabilitation therapy. After 12 months of intervention, the patients in the observation group were treated with cognitive behavioral therapy. The treatment efficiency, psychological status, coping style and medication compliance of the two groups were compared.
Results:
After intervention the scores of Self-rating Depression Scale (SDS) and Hamilton Depression Scale (HAMD) in the observation group were (31.08±4.83), (14.14±3.52) points respectively, which were significantly lower than (36.97±2.73), (16.68 ±2.14) points of the control group (
6.Effects of immune activation on synaptic structure and DISC1 gene expression in cortex of rat with schizophrenia
Chanyuan Pan ; Jing Zhang ; Xinyuan Wang ; Feifei Feng ; Linli Feng
Acta Universitatis Medicinalis Anhui 2023;58(10):1695-1700
Objective :
To study the effect of lipopolysaccharide ( LPS) -induced immune system activation on syn- aptic structure and DISC1.
Methods :
40 rats were divided into 4 groups,which were PBS group,PBS + MK-801 group,LPS group and LPS + MK-801 group.The rats in LPS group and LPS + MK-801 group were administrated with LPS solution (0. 2 mg / kg,i.p.BIW) for 6 times in total, the rats in PBS group and PBS + MK801 group were administrated with PBS.At the third time when the rats were administrated with LPS solution,the rats in PBS + MK-801 group and LPS + MK-801 group were administrated with MK-801 solution (0. 5 mg / kg,i.p.BIW) for a total of 4 times,the rats in PBS group and LPS group were adminis- trated with saline.The head shaking and the open field tests were then evaluated in the rats.Immediately,the rats were sacrificed,and their brain were saved for testing. RT-qPCR , Golgi staining,immunohistochemistry and ELISA kits were used to detect DISC1 gene expression,dendritic spine status,microglia activation and inflammatory cytokine content in cortex,respectively. gene expression in the cortex of rats with schizophrenia.
Results :
Compared with the PBS group,the expression of DISC1 gene of the rats in the PBS + MK-801 group increased (P <0. 01) ,the score of head shaking increased (P <0. 01) ,and the distance of the open field increased (P <0. 000 1) ; As well as the LPS + MK-801 group,compared with the LPS group,the expression of DISC1 gene increased (P<0. 000 1) ,the head shaking score and the distance of the open field increased (P<0. 000 1) . In addition,compared with the PBS + MK-801 group,the expression of DISC1 gene, the shaking head score and the open field distance of the LPS + MK-801 group further improved (P <0. 000 1) . Furthermore,compared with PBS group and PBS + MK-801 group,the content of iba1 in the cortex of LPS group and LPS + MK-801 group significantly increased (P <0. 05 ) ,the content of inflammatory cytokines significantly increased (P<0. 05) ,and the length and density of dendritic spines significantly decreased (P<0. 05) .
Conclusion
The activation of the immune system induced by LPS can contribute to the susceptibility of schizophrenia model, and the possible mechanism is that it cooperates with MK-801 to increase the expression of DISC1 gene,which ulti- mately leads to abnormal mental behavior in rats.
7.Clinical, endoscopic and pathological features of gastrointestinal and mesenteric reactive nodular fibrous pseudotumor
Jianjun WANG ; Xianxiang WANG ; Ying LYU ; Hongyan WU ; Qi SUN ; Jun CHEN ; Ling NIE ; Anning FENG ; Xiangshan FAN
Chinese Journal of Digestive Endoscopy 2018;35(12):876-879
Objective To study the clinical, endoscopic and pathological features of gastrointestinal and mesenteric reactive nodular fibrous pseudotumor (RNFPT). Methods A retrospective analysis was conducted on data of 24 RNFPT patients in Nanjing Drum Tower Hospital admitted from October 2008 to June 2016. The clinical, endoscopic, pathological and immunohistochemical characteristics were analyzed. Results Among the 24 patients, 16 complained about discomfort in the upper abdomen and 10 had a history of surgery or trauma. Twenty-one had isolated masses and 3 had multiple masses, with diameter of 0. 5-4. 0 cm. Endoscopically, the tumors were mainly hard submucosal masses with broad base, and smooth surface with no mucosal bridge. Seventeen patients underwent endoscopic ultrasonography, which showed low echoes in lesions and nonuniform echoes partly. Among them, 13 lesions derived from muscularis, 4 others from submucosa. Microscopically, the tumors had clear boundary with no envelope, and most areas showed disorderly arranged spindle cells and extensively collagenous degenerated mesenchyma. The spindle cells had shuttle fibroblast-like morphology and elongated nucleus with no visible necrosis or mitosis. Inflammatory cells scattered between the tumor cells, and lymphoid follicles and calcium deposition could be seen in local areas. Immunohistochemically, SMA was focally positive in 7 cases and only 4 cases expressed CD117 scattered. Desmin, Dog-1, CD34, ALK-1 and S-100 were all negative, and Ki-67 proliferation index was lower than 1%. Conclusion RNFPT has diverse clinical manifestations, with a good prognosis and unlikely recurrences, and should be distinguished from spindle cell tumors.
8.Reduction of Interhemispheric Functional Connectivity in Sensorimotor and Visual Information Processing Pathways in Schizophrenia.
Xu LANG ; Le WANG ; Chuan-Jun ZHUO ; Feng JIA ; Li-Na WANG ; Chun-Li WANG
Chinese Medical Journal 2016;129(20):2422-2426
BACKGROUNDPrevious studies have demonstrated interhemispheric functional connectivity alterations in schizophrenia. However, the relationship between these alterations and the disease state of schizophrenia is largely unknown. Therefore, we aimed to investigate this relationship using voxel-mirrored homotopic connectivity (VMHC) method.
METHODSThis study enrolled 36 schizophrenia patients with complete remission, 58 schizophrenia patients with incomplete remission and 55 healthy controls. The VMHC was calculated based on resting-state functional magnetic resonance imaging data. Differences in VMHC among three groups were compared using one-way analysis of variance. A brain region with a significant difference in VMHC was defined as a region of interest (ROI), and the mean VMHC value in the ROI was extracted for the post hoc analysis, i.e., pair-wise comparisons across the three groups.
RESULTSVMHC in the visual region (inferior occipital and fusiform gyri) and the sensorimotor region (paracentral lobule) showed significant differences among the three groups (P < 0.05, a false discovery rate method corrected). Pair-wise comparisons in the post hoc analysis showed that VMHC of the visual and sensorimotor regions in schizophrenia patients with complete remission and incomplete remission was lower than that in healthy controls (P < 0.05, Bonferroni corrected); however, there was no significant difference between the two patient subgroups.
CONCLUSIONSInterhemispheric functional connectivity in the sensorimotor and visual processing pathways was reduced in patients with schizophrenia, but this reduction was unrelated to the disease state; thus, this reduction may serve as a trait marker of schizophrenia.
Adult ; Brain ; physiology ; Brain Mapping ; Female ; Humans ; Image Processing, Computer-Assisted ; Magnetic Resonance Imaging ; Male ; Schizophrenia ; physiopathology ; Sensorimotor Cortex ; physiology ; Visual Pathways ; physiology
9.Diagnostic utility of electromagnetic navigation bronchoscopy combined with radial endobronchial ultrasound in peripheral pulmonary lesions
Min YU ; Shenyun SHI ; Yan LI ; Yanzhe YU ; Tingting ZHAO ; Qingqing XU ; Qi ZHAO ; Jingjing DING ; Anning FENG ; Jinghong DAI ; Yonglong XIAO
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery 2022;29(01):44-49
Objective To investigate the diagnostic value and safety of electromagnetic navigation bronchoscopy combined with radial endobronchial ultrasound in peripheral pulmonary nodules. Methods The clinical imaging, surgical and pathological data of 60 patients with 76 peripheral pulmonary nodules who underwent electromagnetic navigation bronchoscopy combined with radial endobronchial ultrasound guided biopsy in the Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School from June 2020 to June 2021 were retrospectively analyzed. The diagnosis rate and complications were analyzed and summarized. The 76 pulmonary nodules were divided into a small pulmonary nodules group (10 nodules, diameter≤1 cm) and a pulmonary nodules group (1 cm
10.Expert consensus on clinical application of 177Lu-prostate specific membrane antigen radio-ligand therapy in prostate cancer
Guobing LIU ; Weihai ZHUO ; Yushen GU ; Zhi YANG ; Yue CHEN ; Wei FAN ; Jianming GUO ; Jian TAN ; Xiaohua ZHU ; Li HUO ; Xiaoli LAN ; Biao LI ; Weibing MIAO ; Shaoli SONG ; Hao XU ; Rong TIAN ; Quanyong LUO ; Feng WANG ; Xuemei WANG ; Aimin YANG ; Dong DAI ; Zhiyong DENG ; Jinhua ZHAO ; Xiaoliang CHEN ; Yan FAN ; Zairong GAO ; Xingmin HAN ; Ningyi JIANG ; Anren KUANG ; Yansong LIN ; Fugeng LIU ; Cen LOU ; Xinhui SU ; Lijun TANG ; Hui WANG ; Xinlu WANG ; Fuzhou YANG ; Hui YANG ; Xinming ZHAO ; Bo YANG ; Xiaodong HUANG ; Jiliang CHEN ; Sijin LI ; Jing WANG ; Yaming LI ; Hongcheng SHI
Chinese Journal of Clinical Medicine 2024;31(5):844-850
177Lu- prostate specific membrane antigen (PSMA) radio-ligand therapy has been approved abroad for advanced prostate cancer and has been in several clinical trials in China. Based on domestic clinical practice and experimental data and referred to international experience and viewpoints, the expert group forms a consensus on the clinical application of 177Lu-PSMA radio-ligand therapy in prostate cancer to guide clinical practice.