Objective To explore prognostic factors of the uterine papillary serous carcinoma (UPSC).Methods Twenty eight patients with UPSC in Cancer Hospital of Shantou University Medical College from January 2002 to August 2013 were analyzed retrospectively.Prognostic factors were analyzed by univariate analysis.Results Twenty eight cases of UPSC with 6.3％ occurrence in the endometrial carcinoma admitted in the same period,including 14 cases of staging Ⅰ ～ Ⅱ,and 14 cases of staging Ⅲ.The univariate analysis showed that lymph-node metastasis and operative-pathological staging were significantly associated with the prognosis (P ＜ 0.05),but were not associated with the menopausal,postoperative treatment,operation style,deep myometrial invasion,cervical involvement,and annex invasion (P ＞ 0.05).Two cases of Ia-staging without myometrial infiltration and without added postoperative treatment were still alive.Conclusions Operative-pathological staging and retroperitoneal lymphadenectomy were independently prognostic factors of UPSC.The Ia-staging without myometrial infiltration might be without added postoperative treatment.

Objective To evaluate the clinicopathological characteristics and prognosis of ovarian mucinous adenocarcinoma.Methods The clinical,pathologic and follow-up data of 39 cases with ovarian mucinous adenocarcinoma were analyzed retrospectively.Results 39 patients with ovarian mucinous adenocarcinoma had a median age of 45 years at diagnosis.The percentage of patients on stage Ⅰ was 59 ％ (23/39),and all patients had the symptoms of ovarian cystic neoplasm or solid mass.There were 24,12,3 cases in grade Ⅰ,Ⅱ,Ⅲ,respectively.Among 15 cases recieved appendectomy,2 cases were appendix infiltrated.The 3-year survival rate of the patients was 63 ％.The result of single-factor analysis showed that the FIGO stage and residual disease after surgery were the correlative factors both in progression free survival (PFS) and overall survival (OS).The result of Cox proportional hazards regression model analysis indicated that residual disease after surgery was the independent prognostic factor of ovarian mucinous adenocarcinoma.Conclusion Ovarian mucinous adenocarcinoma has a better prognosis than other pathological types of ovarian epithelial carcinoma.Appendectomy should be suggested during the surgical treatment of ovarian mucinous adenocarcinoma.The FIGO stage and residual disease after surgery are the important prognostic factors of ovarian mucinous adenocarcinoma.

Objective To evaluate clinical efficacy and survival outcomes of neoadjuvant treatment on early stage bulky cervical carcinoma.Methods A total of 155 cases with bulky stage Ⅰ b2 or Ⅱ a2 cervical carcinoma in Affiliated Tumor Hospital of Shantou University Medical College from Nov.2010 to Feb.2015 was reviewed and divided into two groups according to pre-operative treatment 108 cases in the neoadjuvant treatment group and 47 cases in the control group who underwent radical surgery directly.The clinical,pathologic,and follow-up data were analyzed retrospectively.Results The total response rate of neoadjuvant treatment was 75.0％.Histological grade in neoadjuvant treatment group was better than the other group and the rate of deep myometrial infiltration was tend to low in neoadjuvant treatment group.There was no difference in pathological type,parametrial involvement,lymph node metastasis,and lymph vascular space invasion between two groups.The rate of postoperative treatment was similar (92.6％ vs 87.2％,P ＞ 0.05).The overall survival rate of neoadjuvant treatment group was 96.4％ and the other group was 88.9％ (P =0.069).Conclusions Neoadjuvant treatment was effective for early stage bulky cervical carcinoma.It might reduce tumor histological grade and maybe improve the overall survival of patients.

Objective To explore the value of serum CA125 level to the prognosis of epithelial ovarian cancer.Methods The clinicopathologic data and serum CA125 level of 76 patients with epithelial ovarian cancer from Jan.2003 to Dec.2009 were analyzed retrospectively, and the outcomes of patients were followed up.Results No relationship was found between CA125 concentration before therapy with prognosis and cytoreductive condition for epithelial ovarian cancer.The normal or abnormal CA125 concentration after 3 courses of treatment meant different prognosis, with the 5-year survival rates of 63.8 ％ and 25.0 ％, and the median survival time of 57 months and 28 months, respectively (x2 =19.951, P =0.000).The 5-year survival rates of patients with CA125 concentration≤ 10.0 U/ml and＞10.0 U/ml after therapy were 72.7 ％ and 42.8 ％, respectively (x2 =7.266, P =0.007).According to multivariate Cox proportional-hazard model, two independent factors , operative pathological staging and CA125 concentration after 3 courses, were related significantly to the prognosis of epithelial ovarian cancer (P ＜ 0.05).Conclusion Serum CA125 level, especially after 3 courses and therapy, plays an important role in the prognosis of epithelial ovarian cancer.

OBJECTIVETo investigate the arbuscular mycorrhizal (AM) fungi resources in rhizosphere of Chaenomeles speciosa in Xuancheng, Anhui province.

METHODRoots were stained with acid fuchsin and then mounted and observed under a microscope; Spores were extracted from the rhizosphere soil using wet-sieving method.

RESULTC. speciosa could be colonized and formed typical arbuscular mycorrhizas with AM fingi. 18 species of arbuscular mycorrhizal fungi were identified, belonging to four genera, 11 species of Glomus, 3 species of Acaulospora, 3 species of Scutellospora and 1 species of Entrophospora. Glomus were the dominant AM fungi in the rhizosphere.

CONCLUSIONThe resources of AM fungi in rhiszophere of C. speciosa were very abundant. Fungi from Glomus were possible the dominant AMF in the rhizosphere.

Objective To investigate the inhibitory effects of tetramethoxystilbene, a selective CYP1B1 inhibitor, on adipogenic differentiation of C3H10T1/2 multi-potent mesenchymal cells. Methods In vitro cultured C3H10T1/2 cells at full confluence were induced by adipogenic agents (10μg/ml insulin, 2μmol/L dexamethasone and 0.5 mmol/L 3-isobutyl-1-methylxanthine) and exposed simultaneously to TMS at the final concentrations of 1.0, 2.0 or 4.0μg/ml. Oil Red-O staining was used to observe the cell differentiation. The expression of peroxisome proliferator-activated receptor gamma (PPARγ) and its target genes cluster of differentiation 36 (CD36) and fatty acid binding protein 4 (FABP4) were quantified by real-time RT-PCR and Western blotting. Results Oil Red-O staining and TG contents revealed that TMS suppressed induced differentiation of C3H10T1/2 cells. TMS exposure of the cells dose-dependently decreased both mRNA and protein expressions of PPARγ, a key nuclear transcription factor during adipogenesis, and also lowered the mRNA expressions of PPARγ target genes CD36 and FABP4. Conclusion TMS can suppress adipogenic differentiation of C3H10T1/2 cells by inhibiting PPARγ.

Objective To investigate the inhibitory effects of tetramethoxystilbene, a selective CYP1B1 inhibitor, on adipogenic differentiation of C3H10T1/2 multi-potent mesenchymal cells. Methods In vitro cultured C3H10T1/2 cells at full confluence were induced by adipogenic agents (10μg/ml insulin, 2μmol/L dexamethasone and 0.5 mmol/L 3-isobutyl-1-methylxanthine) and exposed simultaneously to TMS at the final concentrations of 1.0, 2.0 or 4.0μg/ml. Oil Red-O staining was used to observe the cell differentiation. The expression of peroxisome proliferator-activated receptor gamma (PPARγ) and its target genes cluster of differentiation 36 (CD36) and fatty acid binding protein 4 (FABP4) were quantified by real-time RT-PCR and Western blotting. Results Oil Red-O staining and TG contents revealed that TMS suppressed induced differentiation of C3H10T1/2 cells. TMS exposure of the cells dose-dependently decreased both mRNA and protein expressions of PPARγ, a key nuclear transcription factor during adipogenesis, and also lowered the mRNA expressions of PPARγ target genes CD36 and FABP4. Conclusion TMS can suppress adipogenic differentiation of C3H10T1/2 cells by inhibiting PPARγ.

Objective To evaluate the clinicopathological characteristics and prognosis of cervical adenocarcinoma patients with stage Ⅰ b1-Ⅱ b treated with radical hysterectomy and systematic lymphadenectomy.Methods The clinical,pathologic and follow-up data of 118 cases of cervical adenocarcinoma with stage Ⅰ b1-Ⅱ b treated with radical hysterectomy and systematic lymphadenectomy in Cancer Hospital of Shantou University Medical College from Dec.2003 to Nov.2015 were analyzed retrospectively.Results 118 patients of cervical adenocarcinoma with stage Ⅰ b1-Ⅱ b had a median age of 46 years at diagnosis.28 cases were postmenopausal and 90 cases were premenopausal.There were 77 cases in phase Ⅰ b1,25 in stage Ⅰ b2,4 in stage Ⅱ a1,7 in stage Ⅱ a2,5 in stage Ⅱ b.The rate of ovarian metastasis was 3.39％.The 3-year recurrence-free survival rate of the patients was 81.6％.The 3-year overall survival rate of the patients was 83.9％,and 89.10％ for stage Ⅰ b1,73.7％for stage Ⅰ b2,100％ for stage Ⅱ a1,83.3％ for stage Ⅱ a2,60.0％ for stage Ⅱ b.The 3-year overall survival rates of the patients who receive non-chemoradiotherapy,chemotherapy,radiotherapy and chemoradiotherapy after operation were 90.6％,100％,84.6％ and 70％ respectively.The result of Cox proportional hazards regression model analysis indicated that lymph lode metastasis and ovarian metastasis was the independent prognostic factor of desease-free survival,ovarian metastasis and deep myometrial invasion was the independent prognostic factor of overall survival.Conclusions Premenopausal cases are more common than postmenopausal cases in cervical adenocarcinoma with stage Ⅰ b1-Ⅱ b.Ovarian presevation is feasible for early-stage cervical denocarcinoma after full assessment.Pelvic irradiation with concurrent chemotherapy can lead a better prognosis for the patients with pathological risk factors.

To address the increasing need for detecting and validating protein biomarkers in clinical specimens, mass spectrometry (MS)-based targeted proteomic techniques, including the selected reaction monitoring (SRM), parallel reaction monitoring (PRM), and massively parallel data-independent acquisition (DIA), have been developed. For optimal performance, they require the fragment ion spectra of targeted peptides as prior knowledge. In this report, we describe a MS pipe-line and spectral resource to support targeted proteomics studies for human tissue samples. To build the spectral resource, we integrated common open-source MS computational tools to assemble a freely accessible computational workflow based on Docker. We then applied the workflow to gen-erate DPHL, a comprehensive DIA pan-human library, from 1096 data-dependent acquisition (DDA) MS raw files for 16 types of cancer samples. This extensive spectral resource was then applied to a proteomic study of 17 prostate cancer (PCa) patients. Thereafter, PRM validation was applied to a larger study of 57 PCa patients and the differential expression of three proteins in prostate tumor was validated. As a second application, the DPHL spectral resource was applied to a study consisting of plasma samples from 19 diffuse large B cell lymphoma (DLBCL) patients and 18 healthy control subjects. Differentially expressed proteins between DLBCL patients and healthy control subjects were detected by DIA-MS and confirmed by PRM. These data demonstrate that the DPHL supports DIA and PRM MS pipelines for robust protein biomarker discovery. DPHL is freely accessible at https://www.iprox.org/page/project.html?id=IPX0001400000.