1.Promoting effect of connective tissue growth factor on expression of E-cadherin in human Tenon capsule fibroblasts
Chinese Journal of Experimental Ophthalmology 2015;33(8):695-698
Background Scarring of filtration channel following glaucoma filtering surgery is a main cause of the failure of the surgery.The proliferation,epithelial-mesenchymal transition and extracellular matrix remodeling of fibroblasts are thought to be the primary pathological mechanism of scarring.Connective tissue growth factor (CTGF) plays a promoting role in the formation of scar.Whether CTGF participates in mesenchymal-epithelial transition of human Tenon capsule fibroblasts (HTFs) is not clear yet.Objective This study attempted to investigate the effect of CTGF on the mesenchymal-epithelial transition of HTFs in vitro.Methods HTFs were cultured and passaged in high glucose DMEM medium with 10% fetal bovine serum,and the cells of generation 3-6 were used in this experiment.The cells were divided into the blank control group and CTGF-treated group and were routinely cultured in the blank control group.CTGF was added in the medium in the CTGF-treated group,with the final concentration 50 ng/ml CTGF.Immunofluorescence staining was used to identify and locate the expression of E-cadherin protein in the cells,and Western blot assay was employed to quantitatively analyze the expression level of E-cadherin protein in 48 hours after culture.Results The HTFs grew well with the spindle-like shape and vortex-like arrangement.The red fluorescence (E-cadherin protein) in the cytoplasm and blue fluorescence in the cellular nucleus were seen in the CTGF-treated group,but only nucleus with blue fluorescence were obtained in the blank control group.Western blot assay showed that the E-cadherin protein expression was absent in the blank control group,however,the relative expression level of E-cadherin protein in the cells was 0.63± 0.08.Conclusions E-cadherin protein is not expressed in fibroblast derived from mesenchymal tissue.However,CTGF can induce the expression of E-cadherin in HTFs.This study suggests that CTGF promotes the mesenchymal-epithelial transition of HTFs in vitro.
2.Characteristics of CT imaging of adult ischemic moyamoya disease
Anming XIE ; Yaojun DING ; Gongjie LI
China Medical Equipment 2015;(10):12-15
Objective: To study the characteristics of CT imaging of adult ischemic type moyamoya disease, involving CT plain scan, CT perfusion imaging (CTP) and CT angiography (CTA). Methods:A retrospective analysis was made of the imaging data of 20 adult ischemic type moyamoya patients, including CT plain scan, CT perfusion imaging and CT angiography. CT vascular imaging features were graded I-VI with reference to Suzuki vascular grading. Results:Among the 20 patients with adult ischemic type moyamoya , ①CT plain scan: Old infarct lesions occurred in 13 cases(65%), 1 case suffered from acute cerebral infarction(5%), and negative patients totalled 6 (30%);②CT perfusion:5 cases(25%) were normal perfusion, 15 cases(75%) showed obviously low perfusion and local high perfusion;③CT angiography:patients of grade I to VI were respectively 1, 2, 5, 6, 4 and 2. Conclusion:Cerebral infarction lesions associated with adult ischemic type moyamoya disease are distributed mainly in the frontal and parietal cortex, or in watershed regions. Cerebral perfusion is characterized by normal or uneven blood perfusion, especially low perfusion. Vascular imaging manifests mostly degree III and IV, which belong to the middle phase of moyamoya disease.
3.Comparative analysis on the MR imaging characteristics between Ischemic moya-moya ;disease and hemorrhagic moya-moya disease
Anming XIE ; Yaojun DING ; Gongjie LI
China Medical Equipment 2015;(1):35-37,38
Objective: To improve the accuracy of forecasting hemorrhagic moya-moya disease by analyzing the difference in MR imaging between ischemic moya-moya disease and hemorrhagic moya-moya disease. Methods: Retrospective analysis was conducted of clinical and MR imaging data of 64 patients with moya-moya disease between 2009 and 2014 years in Hospital 94 of PLA. Results: Among the 64 patients aged 26 to 49 (average age was 38.2), 21 cases (32.8%) were diagnosed with ischemic moya-moya diseases, while 16 cases (76.2%) diagnosed with hemorrhagic moya-moya diseases, ischemic lesions were distributed mainly in frontal and parietal area, while hemorrhagic lesions were mainly distributed in the dorsal thalamus (28 cases, 65.1%), in the basal ganglia (9 cases, 20.9%), in the simple intraventricular (4cases, 9.3%) and in pure subarachnoid (2 cases, 4.6%). In the ischemic-typed moyamoya disease and hemorrhagic-typed moyamoya disease, cerebral bottom dorsal smoke abnormal vascular network, anterior choroidal artery and callosal artery thickening of the posterior cerebral artery, cortical pial vascular thickening, thickening of vascular branches of ophthalmic artery and external carotid artery thickening were respectively occurred in 15 cases of 28 branch (71.4%) and 38 cases of 62 branches (88.4%), 12 cases with 24 branches (57.1%) and 35 cases with 45 branches (81.4%), 8 cases with 16 branches (38.1%) and 30 cases with 58 branches (69.8%), 5 cases with 10 branches (23.8%) and 13 cases of the 24 branch (30.2%), 7 cases with 11 side branches (33.3%) and 27 patients with 54 branch (62.8%). Conclusion:The tortuous and dilated choroid artery and abnormal hyperplasia vascular network in skull base are the main causes of bleeding in moya-moya diseases.
4.Collateral circulation characteristics of CT angiography imaging of adult ischemic type moyamoya disease
Anming XIE ; Yaojun DING ; Gongjie LI
China Medical Equipment 2016;13(4):64-66,67
Objective:To explore the characteristics of collateral circulation of moyamoya disease in CT angiography imaging.Methods: Data of 120 moyamoya disease patients diagnosed by the 94th hospital were collected. All the patients underwent CT angiography imaging and were divided into groups according to compensatory ways of collateral circulation. Lightspeed VCT was used in all patients to conduct CTA check, and assessed the clinical performance of 4 groups. Results: Group 1 consisted of 15 cases (12.5%) of ischemic type moyamoya disease. Group 2 had 53 cases (44.2%), 8 cases were ischemic type moyamoya disease, and 45 cases were beeding type group moyamoya disease. Group 3 had 38 cases (31.7%), who showed moyamoya vessel formation in the bottom of the brain, 7 of these cases were ischemic moyamoya disease, and 31 were bleeding type moyamoya disease. Group 4 had 14 cases (11.6%), all of whom belonged to bleeding type moyamoya disease, characterized by ophthalmic artery, temporal artery, middle meningeal artery, occipital artery communicating with terminal cortex intracranial vascular.Conclusion: Compensatory characteristics of collateral circulation vessels were closely correlated with the types of moyamoya disease.
6.Identification of Chemical Component Cantharidin from Chinese Blister Beetle by LC-MS/MS
Yaobo ZENG ; Yuyu ZHANG ; Anming TANG ; Dajian YANG ; Chen LI ; Guoqiang LIU ; Yi ZHANG
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(4):876-882
This study was aimed to verify the chemical component cantharidin from Chinese blister beetle by LC-MS/MS. Components were first processed by HPLC to choose the appropriate separation conditions before LC/MS/MS analysis. Through the positive and negative ions pre-scan, positive ions scanning way was selected in the scanning of each separate component. The scanned maps were obtained and the relative molecular mass was deduced. The results showed that 8 types of cantharidin compounds were identified, including three isomers. It was concluded that LC-MS/MS was able to determine the chemical component cantharidin from Chinese blister beetle rapidly and accurately, which was existed in the body by the form of combined amino acid.
7.Correlative factors analysis of affecting anatomical reattachment and vision restoration after scleral buckling surgery
Lei ZHU ; Lin LI ; Xiaoyan TIAN ; Tao LIU ; Meng CHEN ; Pei XU ; Jing ZOU ; Anming XIE
Recent Advances in Ophthalmology 2017;37(2):167-171
Objeetive To analyze the effect of treatment of rhegmatogenous retinal detachment(RRD) by scleral bucking as well as the relative risk factors affecting the anatomical reattachment and visual recovery.Methods One hundred and fortyeight patients (148 eyes) with RRD treated by sclera buckling surgery in our hospital during January 2012 to January 2016 were retrospectively analyzed.The rate of postoperative retinal anatomic reattachment,the best corrected visual acuity (BCVA) and complications were observed.Logistic regression analysis was performed to analyze the correlative factors affecting the anatomical reattachment and postoperative vision restoration.R~ults Retinal reattachment achieved in 91.9% after initial surgery and the final success rate for anatomic reattachment was 97.3% assessed with ophthalmoscope and fundus photography.But these two rates were assessed with the optical coherence tomography (OCT) were 60.1% and 80.4% respectively.Single factor Logistic regression analysis showed that retinal detachment was affected by multiple breaks and Grade C1 PVR(all P <0.05);Single factor Logistic regression analysis showed that preoperative BCVA,course of disease,retinal detachment range,macular involvement or not had an impact on the postoperative recovery of BCVA (all P < 0.05),preoperative age,refractive status,releasing retinal fluid or not,intravitreal gas injection,combined scleral buckling,and postoperative subretinal fluid,all of these factors had no effect on BCVA recovery after surgery (all P > 0.05).And through multiple factors Logistic regression analysis,preoperative BCVA was an independent risk factor for BCVA recovery after surgery (P < 0.05).Conclusion Scleral bucking is an effective technique for managing RRD,but multiple breaks and Grade C1 PVR are significant risk factors for anatomic.Preoperative BCVA,course of disease,retinal detachment range,macular involvement or not have the impact on the BCVA recovery after scleral buckling,and the preoperative BCVA is the key factor.Early diagnosis and early treatment as well as protecting the preoperative visual acuity can improve prognosis.
8.Effects of Myelotomy on Autophagy in Injured Spinal Cord in Rats
Degang YANG ; Jianjun LI ; Mingliang YANG ; Liangjie DU ; Anming HU ; Rui GU ; Liang CHEN ; Hongyu CHU ; Xiandi ZHANG ; Haifeng WU
Chinese Journal of Rehabilitation Theory and Practice 2015;21(4):382-386
Objective To observe the effects of myelotomy on autophagy activation after traumatic spinal cord injury (SCI) in rats. Methods 54 adult female Sprague-Dawley rats were randomly assigned to sham-operated group (SG, n=18), contusion group (CG, n=18) or myelotomy group (MTG, n=18). The T10 SCI model in rats was induced with a New York University (NYU) impactor and myelotomy was performed 24 hours after SCI. They were evaluated with the BBB score 1, 7, 14 days after injury. The expression of mRNA of Beclin-1 and Bcl-2 were detected with real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR). The formation of autophagosome was investigated under electronic microscope (EM) 3 days after injury. Results BBB score was more in the MTG than in the CG 7 and 14 days after injury (P<0.05), while the expression of Beclin-1 mRNA was less (P<0.05). The expression of Bcl-2 mRNA was more in the MTG than in the CG 3 and 7 days after injury (P<0.05). The expression of Beclin-1 mRNA was negatively correlated with BBB scores (P< 0.05). The formation of autophagosome was less in the MTG than in the CG. Conclusion Myelotomy can improve the recovery of motor function in rats after acute traumatic SCI, which may associate with neuroprotection mediated by inhibition of autophagy through the Bcl-2 signaling pathway.
9.Myelotomy suppresses autophagic marker LC3-II expression and elevates mTORC1 expression and improves neurological function recovery in rats with gical function recovery in rats with
Degang Yang ; Jianjun Li ; Rui Gu ; Anming Hu ; Mingliang Yang ; Liangjie Du ; Xin Zhang ; Wei Sun ; Feng Gao ; Yingying Wu ; Jiangen He ; Yutong Feng ; Hongyu Chu
Neurology Asia 2013;18(4):401-407
Although previous studies have shown functional efficacy of myelotomy for the treatment of spinal
cord injury (SCI), the underlying mechanism remained unknown. This study aimed to determine
the relationship between myelotomy-mediated neuroprotection and autophagy following SCI by
evaluating the expression of microtubule-associated protein light chain 3 (LC3-II) and mammalian
target of rapamycin complex 1 (mTORC1). Ninety-nine adult female rats were randomly assigned
to either sham-operated group (SG), model group (MG), or 24 h-myelotomy group (MTG). SCI at
T10 was induced with a New York University impactor, and myelotomy was performed 24 h after
SCI. Functional recovery was evaluated via the open-field test. The protein expression of LC3-II
was analyzed by Western blot, and the mRNA expression of LC3-II and mTORC1 were detected by
real-time quantitative reverse transcriptase polymerase chain reaction. Rats in the MTG exhibited
significantly better performance in the hind limbs compared to those in the MG on day seven and
fourteen post-injury. Myelotomy suppressed the protein and mRNA expression of LC3-II on day three,
seven and fourteen post-injury and increased the mRNA expression of mTORC1 in the MTG on day
three and seven post-injury. The LC3-II protein expression was significantly and negatively correlated
with BBB scores at day seven and fourteen post-injury. These results showed that myelotomy-induced
neuroprotection in a rat model of SCI was likely mediated by inhibition of autophagy by activation
of the mTORC1 signaling pathway
10.Clinical study of self-designed Yiqi Huoxue Huayu Decoction combined with western conventional therapy in the treatment of chronic heart failure complicated with atrial fibrillation
Lixiao WANG ; Xiaoli LI ; Haijun TANG ; Qiang PU ; Jingwei WANG ; Leilei XU ; Anming SHEN
International Journal of Traditional Chinese Medicine 2022;44(7):749-753
Objective:To observe the effects of self-designed Yiqi Huoxue Huayu Decoction on cardiac function and serum endothelin (ET-1) and matrix metalloproteinase-9 (MMP-9) levels in patients with chronic heart failure (GHF) complicated with atrial fibrillation.Methods:A total of 100 patients with GHF complicated with atrial fibrillation treated in our hospital from January 2019 to June 2021 were selected as the study subjects, and divided into experimental group and control group according to random number table method, with 50 patients in each group. Patients in both groups were given cardiotonic, diuretic, vasodilator and other conventional treatment, and patients in the experimental group added self-designed Yiqi Huoxue Huayu Decoction. TCM syndrome score, left ventricular end diastolic diameter (LVEDD), left ventricular end systolic diameter (LVESD) and left ventricular ejection fraction (LVEF), serum ET-1 and MMP-9 levels, clinical efficacy, safety and incidence of adverse reactions were observed and compared between 2 groups before and after treatment.Results:The total response rate was 82.0% (41/50) in the observation group and 62.0% (31/50) in the control group, and the difference was statistically significant ( χ 2=4.96, P=0.026). After treatment, the symptoms scores of palpitation, shortness of breath, fatigue, chest and hypochasm pain in the observation group were significantly significantly lower than those in the control group ( t=5.28, 5.29, 5.62, 5.42, P<0.01). After treatment, the LVEDD[(51.23±6.59)mm vs. (55.65±6.17)mm, t=3.46], LVESD[(43.10±4.76)mm vs. (45.99±5.31)mm, t=2.87], serum ET-1[(65.79±8.29)μg/L vs. (79.83±10.08)μg/L, t=7.61], MMP-9 [(175.86±24.81)ng/L vs. (189.49±26.13)ng/L, t=2.68] in experimental group were significantly lower than those in the control group ( P<0.05), while LVEF [(50.01±7.6)% vs. (46.25±6.96)%, t=2.57] was significantly higher than that of the control group ( P<0.05). There were no significant differences in GPT, GOT, UA, SCr levels between both groups before and after treatment ( P>0.05). There were no obvious adverse reactions betewwn both groups. Conclusion:The self-designed Yiqi Huoxue Huayu Decoction can relieve the clinical symptoms, improve the heart function and serum ET-1 and MMP-9 levels in patients with GHF complicated with atrial fibrillation safely.