Objective To investigate clinical and MRI features of the reversible posterior encephalopathy syndrome (RPES).Methods Clinical and MRI materials of 4 RPES cases confirmed by integrated clinical diagnosis were retrospectively analyzed.Results All the cases presented with a rise of blood pressure.The neural symptom include severe headache (2 cases),dysphoria (3 cases),blur of vision (2 cases),impairment of impermanent memory (1 case),seizure (2 cases).In MRI,the lesions mainly systematically located in posterior parietal lobe and occipital lobe in all cases,or randomly located in frontal lobe (2 cases).Lesions demonstrated high signal on T_2 weighed and water-suppressed sequences in MRI.The lesions dominantly located in cortex in 2 cases,in both cortex and subcortieal white matter in 1 case and in subcortical white matter in 1 case.Four cases resolved their lesions when re-examined. Conclusions RPES represents such characteristic MRI findings as symmetrical distribution of reversible lesions in posterior brain with involvement of cortex or subcortieal white matter.When combined with hypertension and neural symptoms caused by involvement of posterior brain structure,it is helpful for early differential diagnosis of this syndrome.

Aim To prepare and identify monodonal sntibody (Mab) specific for Toaoplasma gondii tachyzoites. Method The Mab specific for Taxoplasma gondii tachyrzoite were prepared via bybridoma technique. Indirect ELISA was used to determine the activity of the Mab. Agarose double immuodiffusion test was performed to confirm subclass and SDS-PAGE & western blot were used to analysis rolecular weight of the antigen (s) recognized by the Mab. IFA was used to identify the epitope of Taxoplasma gondii tachyzoites. The protection and specificity of the Mab were snalysed at same time. The Mab was tesed in Mab-ELISA method to detect Taxoplasma gondii antigen. Results A Msb F7C8H12 specific for T. gondii was produced. It belongs to IgG1 subclass. Moleculsr weight of the sntigens recognized by the Mab was 16.5 and 24 kDa. IFA did not show fiuorescence in intact tachyzoite.Inhibition test showed that the inhibition rate was 50% when the concetration of the antigen was 40μg/ml.Afterthe RH strain tachyzoites were incubated with Mab ascites, mice were inected with the tachyzoites through peritoneum. The results showed that the mean dead time of mice were not delayed. T. gondii antigen mixed with PBS snd normal human serum was detected by Mab-ELISA, the sensitvity was 0.78 yg/ml and 1.5μg/ml respectively. When mice were infected with T. gondiiRH strain tachyzoites, 103/mouse p.i., circulating antigen could be detectedin 6 day and 8 day. Conchusion The Mab (F7C8H12) to T. gomdii tachyzoites is an excellent probe for studying T. gondii snd toxoplasmosis.

Objective To Establish evaluation methods for the performance of flow cytometer .Methods Referring to the indus‐try standard YY/T0588‐2005 Flow Cytometry ,evaluating methods for the performance of BriCyte E6 flow cytometry was estab‐lished ,such as fluorescence sensitivity ,fluorescence linearity ,forward scatter sensitivity ,instrument resolution ,forward scatter/side scattering resolution ,DNA content linearity ,carry‐over rate ,accuracy of the cell surface marker ,reproducibility of the cell surface marker and instrument stability .Results The performance of BriCyte E6 met the requirements of industry standard .Conclusion The evaluation methods for the performance parameters could be reliable and could be used for the performance evaluation of flow cytometer .

It has been 50 years since the discovery of thyrotropin receptor autoantibody (TRAb). Advances in the knowledge of thyrotropin receptor ( TSHR) structure and function, combined with the elucidation of TSHR signaling and TSHR-autoantibody interaction have greatly facilitated our understanding of TRAb and their clinical applications. Measurement of TRAb activity plays an important role in the diagnosis of Graves' disease ( GD) and Graves' opthalmopathy. It has also been well recognized that TRAb is an effective predictor of GD relapse or remission after antithyroid drug and radioactive iodine treatment. TRAb test is of particular help in pregnant women and lactating mothers with recent iodine load, where radioactive iodine or technetium tests are contraindicated. In addition, it is useful in the diagnosis and differential diagnosis of fetal and neonatal hyperthyroidism as well as some rare forms of thyrotoxicosis in clinical practice. Accumulating evidence also indicates the possible correlation between thyroid cancer occurring in GD patients with positive TRAb and adverse outcomes. However, further innovation and standardization of TRAb tests are required to help pave the way for clinical applications.

Objective To construct eukaryotic expression vector of HPV18 L1- E6, E7 chimeric gene and examine the humoral and cellular immune responses induced by this DNA vaccines in mice. Methods The C-terminal of major capsid protein L1 gene and mutant zinc finger domains of early E6/7 oncogenes in HPV18 were integrated and inserted into eukaryotic expression vector pVAX1 to generate vaccines pVAX1-L1E6Mxx, E7Mxx. CHO cells were transiently transfected with the individual construct. Target protein expressions in the lysate of the transfected cells were measured by ELISA and immunocytochemistry. After BALB/c mice were vaccinated with various recombinant plasmids(pVAX1-L1-E6M3 or pVAX1-L1-E7M3) and immunie adjuvants (pLXHDmB7-2 or LTB) through different administration routes (intramuscular or intranasal) , the great cellular immune responses were produced as revealed by delayed-type hypersensitivity (DTH) and lymphocyte proliferation, and the expression of IL-4 and IFN- γ cells in CD4+ and CD8+subpopulations. Results The highly efficient expression of pVAX1-L1E6Mxx, E7Mxx vector in host eukaryotic cells were demonstrated both by ELISA and immunocytochemistry. The level of specific serum IgG against HPV in experiment groups mice was much higher than that of control group, and intranuscular immunization group had the highest antibody level. Intramuscular immunization groups were superior to intranasal immunization groups in DTH response, splenocyte proliferation and CD8+ IFN-γ + cells number, but CD4+ IL4+ cell number was higher in intranasal immunization groups. The immunization groups using pLXHDmB7-2 as adjuvant were superior to other groups in immunoresponse. Conclusion These DNA vaccines produce remarkable cellular and humoral immuneresponses in the mouse and may provide as prophylatic and therapeutic candidates for HPV induced cancer treatment.

PURPOSE: The purpose of this study was to identify the effects of bullying, negative affectivity and burnout in the nurses. METHODS: The sample consisted of 389 nurses. Data were analyzed using frequency, percentage, mean, standard deviation, t-test, ANOVA, Scheffe, Pearson Correlation Analysis and Hierarchical Multiple Regression. RESULTS: The control variables of age, marital status, education level, current position, health status and work unit explained 30.2%(F=28.9, p<.001) of variance in burnout. The control variables, bullying and negative affectivity collectively explained 49.7% of variance in burnout. CONCLUSION: The results indicate that the demographic factors influencing burnout are age, education level, health status, work unit, while bullying and negative affectivity in the work places are factors that influence burnout. These findings can be utilized to develop strategies to reduce bullying and negative affectivity.
Bullying* ; Demography ; Education ; Marital Status ; Workplace

Medulloepithelioma(diktyoma) is a uncommon congenital tumor that is generally believed to arise from the embryonic neuroepithelium of the medullary tube. This tumor usually arises from the ciliary body epithelium. But medulloepitheliomas of the retina and optic nerve may also occur, although it is extremely rare, because the embryonal medullary epithelium that lines the optic vesicle is continuous with the epithelium lining the cavity of the optic nerve during the early embryonal period. The occurrence of medulloepithelioma of the optic nerve is exceptionally rare, with only a few cases have been previously reported. We report a first case of medulloep-ithelioma of the optic nerve head in Korea in a 5-year-old girl who presented with a poor vision and squint of the left eye.
Child, Preschool ; Ciliary Body ; Epithelium ; Female ; Humans ; Korea ; Neuroectodermal Tumors, Primitive* ; Optic Disk* ; Optic Nerve* ; Retina ; Strabismus

PURPOSE: Medical institutions and their patients benefit from continued employment of nurses. In this study an assessment was done of important factors that influence nurses' intention to leaving their jobs. METHODS: The sample consisted of 229 university hospital nurses. Data were analyzed using frequency, percentage, mean, standard deviation, t-test, ANOVA, Scheffé, Pearson's Correlation Analysis, and Hierarchical Multiple Regression. RESULTS: The control variables, including age, current position, and health status explained 18% (F=16.37, p<.001) of variance in retention intention. The control variables, bullying, job stress, self-esteem, and burnout collectively explained 27% of variance in retention intention. CONCLUSION: The findings indicate that the factors influencing retention intention are age, current position, and health status, while self-esteem and burnout in work places are new and more recent factors that impact retention intention. These findings can be utilized to develop strategies to increase self-esteem and retention intention.
Bullying* ; Employment ; Humans ; Intention* ; Nursing* ; Workplace

It seems best to view ADHD as a developmental disability that comprises impairments in persistence of effort(sustained attention), the ability to delay responding and inhibit behavior(impusivity), and the regulation of excessive activity level or responding in situations(hyperactivity). These problems tend to arise early in childhood, often during the preschool years, and persist in over half the cases into adulthood as adult ADHD. ADHD in adolescents and adults responds to treatment with stimulants, tricyclic antidepressants, etc. Nonpharmacologic modalities such as behavior modification, individual and family therapy and cognitive therapy are useful adjuncts to psychopharmacologic management. Without effective treatment, ADHD often results in increased risk of trauma, substance abuse, conduct disorder, marital dishamony, family dysfunction, divorce, and incarceration in adolesence and adulthood. Lifelong treatment with proper medication and counselling are important to ADHD.
Adolescent* ; Adult* ; Antidepressive Agents, Tricyclic ; Behavior Therapy ; Cognitive Therapy ; Conduct Disorder ; Developmental Disabilities ; Divorce ; Drug Therapy ; Family Therapy ; Humans ; Substance-Related Disorders

The criteria for evaluating permanent impairments resulting from principal dysfunction of the ear, nose, throat, and related structures are uncertain in our country. However, our government is now striving to build up a welfare state with upgrading the law of industrial accident insurance. The author assess permanent impairment ratings of these structures by evaluating losses in structures or the following functions: hearing; equilibrium; respiration; mastication; olfaction, and taste; speech and voice; and the effect of these losses on the ability to perform activities of daily living. Impairment criteria were adapted from the literature, law and ordinances in our country.
Accidents, Occupational ; Activities of Daily Living ; Ear ; Hearing ; Insurance ; Jurisprudence ; Mastication ; Nose ; Otolaryngology* ; Pharynx ; Respiration ; Smell ; Voice