Objective The aim is to expore the expression of HSP70 in human esophagus cancer and introduce method of separation of HSP70-peptide compound.Methods The expression of human HSP70 RNA in human(esophagus) cancer was hybridized with P17 probe by Northern blot.The HSP70-peptide complex was isolated by(affinity) chromatography.Results HSP70 mRNA was detected in human esophagus cancer tissue at much more higher level than corresponding normal tissue;HSP70-peptide compound are separated from esophagus cancer of human body.Conclusion This research not only testified the strong expression of HSP70 in esophagus cancer,but also supplied a practical method of extraction and purification of HSP70-peptide compound of tumor origin.This study makes a starting point for further research in this field.

Objective To explore the characteristics of sleep disturbance and its related factors in patients with rheumatoid arthritis (RA).Methods A total of 71 patients with RA in Department of Rheumatology Huaxi Hospital have completed the following questionnaires, including Pittsburgh sleeping quality index (PSQI), visual analogue scale (VAS), disease activity score in 28 joints (DAS28), health assessment questionnaire (HAQ), hospital anxiety and depression scale (HADS), fatigue severity scale (FSS) and a self-designed general status questionnaire.Results The prevalence of sleep disturbance was 42.3％ (30/71) in rheumatoid arthritis patients (68.4％).The scores of DAS28, VAS, PSQI, HAQ, FSS and HADS in patients with sleep disturbance were significantly higher than those in patients with good sleep, which were respectively 3.90 ± 1.12 vs 2.92 ± 1.92, (5.03 ± 2.63) scores vs (2.41 ± 1.84) scores, (10.87 ± 2.42) scores vs (4.29 ± 1.85) scores, 3.0 (0.0, 7.0) scores vs 2.0 (0.5,4.0) scores, (39.17 ±14.02) scores vs (29.63 ± 16.12) scores, (14.50 ±7.77) scores vs (9.49 ±6.57) scores (P ＜ 0.05 in all scales).According to the results of Pearson correlation analysis, PSQI had significantly positive correlation with DAS28 (r =0.462, P ＜ 0.01), VAS (r =0.556, P ＜ 0.01), HAQ (r =0.360, P ＜ 0.01), FSS(r =0.420, P ＜ 0.01) and HADS (r =0.447, P ＜ 0.01) respectively.The logistic regression analysis indicated that VAS was a predictor for poor sleep quality (P ＜ 0.01).The patients receiving biological agents had significantly (P ＜ 0.05) lower scores of DAS28 (2.86±1.39 vs 3.52 ± 1.1 0), PSQI [(5.90 ± 4.24) scores vs (8.53 ± 3.78) scores], VAS(2.15 ± 2.30 vs 4.05 ± 2.46), HAQ [0.0 (0.0, 2.0) scores vs 3.0(0.0,6.0) scores] compared to those taking oral drugs.Conclusion High prevalence of sleep disturbance in patients with RA is noted, which indirectly influences the activity of disease, quality of life, depression, fatigue and other physical and mental health.Biological agents can partly improve the sleep disturbance and functional status.

Objective To study the effects of injection of brain waking-liquid on unconsciouness rat as its outer brain injured. Methods To inject via abdomen the liquid to the testing rats with symptoms. Results The surviving rate and the consciousness regaining rate of every testing group is obviously higher than that of control groups. They have notable differences statistically.Conclusion The brain waking liquid is effective for curing the unconsciouness by outer brain injury.

Objective To investigate the incidence of latent tuberculosis infection (LTBI) in patients with rheumatic diseases in order to find evidence for the prevention of mycobacterium tuberculosis (MTB) in these patients.Methods From January 2013 to July 2015,759 patients with rheumatic diseases and 38 health controls were enrolled.All of them underwent interferon-gamma release assays(T-SPOT.TB) to screen for LTBI.Incidence of MTB infection was evaluated in different groups and test was used for statistical analysis between groups.Results The incidences of LTBI in patients and health controls were 27.27％ (207/759) and 10.53％ (4/38),respectively.In 2013,24.66％ (73/296) (standardized infection rate 23.37％) patients with rheumatic diseases were positive for LTBI screening test.In 2014 and 2015,the percentages were 32.02％ (73/296) (standardized infection rate was 32.15％) and 25.96％ (73/228) (standardized infection rate was 28.46％),respectively,which was statistically significant in these 3 groups (P =0.004).the infection rate in 2014 tended to be higher than that in 2013 (P =0.001).There were 30.24％ (88/291) male and 25.43 ％ (119/468) female patients who were considered as LTBI.But the difference was not significant between genders.The infection rates between patients older than 60 years old and less was significantly different,which were 45.65％ (42/92) and 24.74％ (165/667),respectively (P =0.000).As far as diseases were concerned including rheumatoid arthritis,systemic lupus erythematosus,spondyloarthritis and other rheumatic diseases,the incidences were 33.93％ (57/168),22.06％ (45/204),25.73％ (44/171) and 28.24％ (61/216) respectively,without statistical significance.Conclusions The incidence of LTBI is high in patients with rheumatic diseases.Attention should be paid especially to elderly patients and rheumatoid arthritis patients who have relatively higher rates of LTBI.Careful monitoring and prevention measures are suggested to take in these patients.