A study on hemocyte in peripheral blood of women ages of 18 and above in some suburb communes in Th¸i B×nh province has shown that level of average Hb is about 120 - 130 g/l, highest level of it is in ages of 18 -30 (141, 44 g/l) and lowest in ages of 70 and above (123,66g/l). The rate of subjects with Hb level less than average normal threshold (120% g/l) is 24,76%. This rate is 20,16% in ages of 41 -50 and highest rate is 48,48% in ages of 70 and above mild anemia (most common); moderate anemia: uncommon; and severe anemia: 0.
Hemocytes ; Hemoglobins ; Anemia Peripheral Blood Stem Cell Transplantation

The dose of CD34+ cells is known to influence the outcome of allogeneic peripheral blood stem cell (PBSC) and/or T-cell-depleted transplantation. A previous study proposed that 2×10⁶ CD34+ cells/kg is the ideal minimum dose for allogeneic transplantation, although lower doses did not preclude successful therapy. In the case we present here, CD34+ cells were collected from a matched sibling donor on the day of allogeneic hematopoietic stem cell transplantation; however, the number of cells was not sufficient for transplantation. Consequently, PBSCs were collected three additional times and were infused along with cord blood cells from the donor that were cryopreserved at birth. The cumulative dose of total nuclear cells and CD34+ cells was 15.9×10⁸ cells/kg and 0.95×10⁶ cells/kg, respectively. White blood cells from this patient were engrafted on day 12. In summary, we report successful engraftment after infusion of multiple low doses of CD34+ cells in a patient with severe aplastic anemia.
Anemia, Aplastic ; Cord Blood Stem Cell Transplantation ; Fetal Blood ; Hematopoietic Stem Cell Transplantation ; Humans ; Leukocytes ; Parturition ; Peripheral Blood Stem Cell Transplantation ; Siblings ; Stem Cells ; Tissue Donors ; Transplantation, Homologous

Anemia, Aplastic ; therapy ; Child ; Female ; HLA Antigens ; genetics ; immunology ; Humans ; Mesenchymal Stem Cell Transplantation ; Peripheral Blood Stem Cell Transplantation

Generalized vitiligo associated with allogeneic bone marrow or stem cell transplantation has rarely been reported. We experienced two cases of generalized vitiligo after allogeneic bone marrow and stem cell transplantation. The first patient was received an allogeneic bone marrow transplantation and subsequent allogeneic peripheral blood stem cell transplantation for severe aplastic anemia from his sister. The second patient underwent allogeneic bone marrow transplantation for acute myelogeneous leukemia from his sister. Generalized vitiligo developed after transplantation. Neither vitiligo nor evidences of autoimmune diseases were found in their stem cell donors. Vitiligo was effectively treated with narrow band UVB irradiation.
Anemia, Aplastic ; Autoimmune Diseases ; Bone Marrow ; Bone Marrow Transplantation ; Humans ; Leukemia ; Peripheral Blood Stem Cell Transplantation ; Siblings ; Stem Cell Transplantation* ; Stem Cells* ; Tissue Donors ; Vitiligo*

BACKGROUND: Most hypomethylating agent (HMA) responders with myelodysplastic syndrome (MDS) eventually need allogeneic stem cell transplantation (SCT) because they often acquire resistance to HMAs within two years of treatment. Considering the nature of MDS and the poor outcomes of SCT when performed after confirming the progression of MDS to acute myeloid leukemia (AML), allogeneic SCT should be performed with caution in patients with low-risk MDS. METHODS: To address low-risk MDS, the Korean AML/MDS working party group designed a survey for 34 MDS experts in Korea on therapeutic HMA and allogeneic SCT policies for low-risk MDS. The level of consensus was defined as the percentage of agreement among the experts. RESULTS: With regard to the optimal time for allogeneic SCT for HMA responders with MDS-RA, 76% experts agreed that allogeneic SCT should be performed when a patient has a low platelet count. With regard to the relapse pattern that was most commonly found during HMA treatment in responding patients with MDS-RA, 54% experts agreed that the most common pattern that indicated HMA failure was the gradual worsening of cytopenia. CONCLUSION: The optimal time to perform allogeneic SCT in RA patients who achieved hematologic complete remission during HMA treatment is when the platelet count decreases. However, these suggestions need to be evaluated in larger future studies. Therefore, careful decisions should be taken at each step of allogeneic SCT to maximize the outcomes for patients with MDS-RA and iron overload.
Anemia* ; Consensus ; Humans ; Iron Overload ; Korea ; Leukemia, Myeloid, Acute ; Myelodysplastic Syndromes ; Peripheral Blood Stem Cell Transplantation* ; Platelet Count ; Recurrence ; Stem Cell Transplantation

Lichens striatus (LS) is an acquired, self-limiting inflammatory dermatosis that follows the lines of Blaschko. The etiology of the eruption is unknown, but several theories have been proposed with focus on environmental factors, viral infection, cutaneous injury, hypersensitivity, and genetic predisposition. We describe a 19-year-old woman who developed a unilateral linear eruption 17 months after allogenic peripheral blood stem cell transplantation. Histopathology revealed features, which were consistent with LS. To the best of our knowledge, our patient is the first case describing the appearance of LS occurring after allogenic stem cell transplantation. We speculate that this condition represents an unusual form of localized, chronic graft-versus-host disease.
Adult ; Anemia, Aplastic ; Female ; Genetic Predisposition to Disease ; Graft vs Host Disease ; Humans ; Hypersensitivity ; Lichens ; Peripheral Blood Stem Cell Transplantation ; Skin Diseases ; Stem Cell Transplantation ; Young Adult

PURPOSE: To evaluate the features of magnetic resonance(MR) imaging of bone marrow (BM) following bone marrow transplantation (BMT). MATERIALS AND METHODS: Eighteen BMT recipients (8 severe aplastic anemia and 10 leukemia patients) underwent MR imaging. Fourteen were males and four were females, and their mean age was 27.2 years. Allogeneic transplantation was performed in 14 patients, unrelated transplantation in three, and peripheral blood stem cell transplantation in one. The mean interval between BMT and MR examination was 22.7 weeks. MR imaging was performed using a 0.5 T superconducting MR unit(Gyroscan T5, Phillips, Netherlands). Signal intensity(SI) on T1 weighted (T1WI) and short tau inversion recovery(STIR) images of lumbar vertebral BM, and on T1WI of pelvic BM, was analyzed with respect to that of muscle. RESULTS: In nine patients in whom BMT was successful, the SI of lumbar vertebral BM was low to slightly high on T1WI and iso to low on STIR images. Six patients with labile engraftment syndrome and two relapsed patients showed inhomogeneous high SI with scattered low signal areas on T1WI and variable SI on STIR images. In particular, in patients who had relapsed, the SI seen on STIR images was high. One patient in whom rejection had occurred showed homogeneous high SI on T1WI and low SI on STIR images of lumbar vertebral BM. The SI of pelvic BM, as seen on T1WI, was inhomogeneously high, irrespective of engraftment status. The SI of pelvic BM showed a larger high-signal portion than did lumbar vertebral BM seen on T1WI. CONSLUSION: MR imaging of lumbar vertebral BM was useful for the evaluation of BM status after BMT. Engraftment of the pelvis might be delayed compared to that of the lumbar vertebrae.
Anemia, Aplastic ; Bone Marrow Transplantation* ; Bone Marrow* ; Female ; Humans ; Leukemia ; Lumbar Vertebrae ; Magnetic Resonance Imaging* ; Male ; Pelvis ; Peripheral Blood Stem Cell Transplantation ; Transplantation, Homologous

PURPOSE: To evaluate the features of magnetic resonance(MR) imaging of bone marrow (BM) following bone marrow transplantation (BMT). MATERIALS AND METHODS: Eighteen BMT recipients (8 severe aplastic anemia and 10 leukemia patients) underwent MR imaging. Fourteen were males and four were females, and their mean age was 27.2 years. Allogeneic transplantation was performed in 14 patients, unrelated transplantation in three, and peripheral blood stem cell transplantation in one. The mean interval between BMT and MR examination was 22.7 weeks. MR imaging was performed using a 0.5 T superconducting MR unit(Gyroscan T5, Phillips, Netherlands). Signal intensity(SI) on T1 weighted (T1WI) and short tau inversion recovery(STIR) images of lumbar vertebral BM, and on T1WI of pelvic BM, was analyzed with respect to that of muscle. RESULTS: In nine patients in whom BMT was successful, the SI of lumbar vertebral BM was low to slightly high on T1WI and iso to low on STIR images. Six patients with labile engraftment syndrome and two relapsed patients showed inhomogeneous high SI with scattered low signal areas on T1WI and variable SI on STIR images. In particular, in patients who had relapsed, the SI seen on STIR images was high. One patient in whom rejection had occurred showed homogeneous high SI on T1WI and low SI on STIR images of lumbar vertebral BM. The SI of pelvic BM, as seen on T1WI, was inhomogeneously high, irrespective of engraftment status. The SI of pelvic BM showed a larger high-signal portion than did lumbar vertebral BM seen on T1WI. CONSLUSION: MR imaging of lumbar vertebral BM was useful for the evaluation of BM status after BMT. Engraftment of the pelvis might be delayed compared to that of the lumbar vertebrae.
Anemia, Aplastic ; Bone Marrow Transplantation* ; Bone Marrow* ; Female ; Humans ; Leukemia ; Lumbar Vertebrae ; Magnetic Resonance Imaging* ; Male ; Pelvis ; Peripheral Blood Stem Cell Transplantation ; Transplantation, Homologous

OBJECTIVETo evaluate the efficacy of treatment with allogeneic peripheral blood stem cell transplantation for benzene-induced severe aplastic anemia.

METHODSHLA-compatible sibling (pregnancy) allogeneic peripheral blood stem cell transplantation (Allo-PBSCT) was successfully performed for a patient with severe aplastic anemia caused by benzene poisoning. 9.41 x 10(8) nucleated cells/kg, 12.49 x 10(6) CD(34) positive cells/kg and CFU-GM 8.2 x 10(5)/kg were infused. The patient was treated with cyclophosphamide (120 mg/kg), total body radiation (8 Gy) and anti-lymphocyte globulin (60 mg/kg) before transplantation. Donor buffy coat cells (9.02 x 10(8) nucleated cells/kg, 10.62 x 10(6) CD(34) positive cells/kg, 6.3 x 10(5) CFU-GM/kg) were infused again on day 18 after transplantation to prevent from graft failure. Graft versus host disease prophylaxis consisted of both methotrexate and cyclosporin A.

RESULTSThe lowest ANC was 0, the lowest platelet was 3 x 10(9)/L after transplantation. The patient achieved an ANC of greater than 0.5 x 10(9)/L from 21st day, and the platelet of greater than 50 x 10(9)/L from 28th day after transplantation. Grade I cGVHD was found the fourth month after grafting. Examination of recipient's bone marrow cells showed a normal 46, XX (presumably marrow donor) karyotype. Blood group changed from B to O.

CONCLUSIONThis is the first case reported in China showing a successful treatment of benzene-induced severe aplastic anemia with allo-PBSCT. Allo-PBSCT may be an effective remedy for this kind of patients.

Adult ; Anemia, Aplastic ; chemically induced ; therapy ; Benzene ; poisoning ; Humans ; Male ; Occupational Diseases ; chemically induced ; therapy ; Peripheral Blood Stem Cell Transplantation ; Transplantation, Homologous ; Treatment Outcome

OBJECTIVETo investigate the efficacy of allogeneic peripheral blood stem cell transplantation (PBSCT) in the treatment of severe aplastic anemia (SAA) and severe infection.

METHODSA patient with SAA and pseudomonas aeruginosa septicemia was treated with PBSCT from an HLA-identical sibling with cyclophosphamide (CY) and total body irradiation (TBI) for conditioning. The patient was infused with 20.3 x 10(8)/kg mononuclear cells including 61.0 x 10(6)/kg CD34(+) cells following the conditioning regimen.

RESULTSTwelve days after PBSCT, the absolute neutrophil count (ANC) of 1.0 x 10(9)/L was achieved, with platelet count > 50 x 10(9)/L at twenty days. The donor origin of engraftment was confirmed by polymerase chain reaction (PCR) analysis of short tandem repeats at the end of the first, sixth and twelfth month. The patient's body temperature dropped to normal level when her ANC reached 0.5 x 10(9)/L on day 10, and the bacterial culture of blood sample became negative subsequently. Symptoms and signs of acute or chronic graft versus host disease (GVHD) were not observed in 30 months after PBSCT.

CONCLUSIONSHematopoiesis was reconstituted shortly after PBSCT. The combination of CY and TBI and the infusion of sufficient peripheral blood stem cells may contribute to the successful engraftment. PBSCT may be considered as the first choice when hematopoietic stem cell transplantation is needed for SAA patients complicated with severe infection.

Adult ; Anemia, Aplastic ; immunology ; therapy ; Bacteremia ; complications ; Female ; Humans ; Peripheral Blood Stem Cell Transplantation ; methods ; Prognosis ; Pseudomonas Infections ; complications ; Transplantation, Homologous ; immunology