Aged ; Angiofibroma ; metabolism ; pathology ; Desmin ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Inflammation ; Leiomyosarcoma ; pathology ; Neoplasms, Muscle Tissue ; metabolism ; pathology ; Vimentin ; metabolism ; Vulvar Neoplasms ; metabolism ; pathology

When people are walking, they will produce gait signals and different people will produce different gait signals. The research of the gait signal complexity is really of great significance for medicine. By calculating people's gait signal complexity, we can assess a person's health status and thus timely detect and diagnose diseases. In this study, the Jensen-Shannon divergence (JSD), the method of complexity analysis, was used to calculate the complexity of gait signal in the healthy elderly, healthy young people and patients with Parkinson's disease. Then we detected the experimental data by variance detection. The results showed that the difference among the complexity of the three gait signals was great. Through this research, we have got gait signal complexity range of patients with Parkinson's disease, the healthy elderly and healthy young people, respectively, which would provide an important basis for clinical diagnosis.
Aged ; Gait ; Healthy Volunteers ; Humans ; Parkinson Disease ; physiopathology ; Reference Values ; Signal Processing, Computer-Assisted ; Walking

OBJECTVEThis study evaluates the expression of interleukin-18 (IL-18) and hypoxia-inducible factor (HIF)-lα in rat periodontitis model exposed to normoxia and chronic intermittent hypoxia (CIH) environments. The possible correlation between periodontitis and obstructive sleep apnea-hypopnea syndrome (OSAHS) was also investigated. Methods: Thirty-two Sprague-Dawley (SD) rats were randomly assigned into four groups: normoxia control, normoxia periodontitis, hypoxia control, and hypoxia periodontitis groups. The periodontitis models were established by ligating the bilateral maxillary second molars and employing high-carbohydrate diets. Rats in hypoxia control and hypoxia periodontitis groups were exposed to CIH treatment mimicking a moderately severe OSAHS condition. All animals were sacrificed after eight weeks, and the clinical periodontal indexes were detected. The levels of IL-18 and HIF-1α in serum and gingival tissues were determined using enzyme-linked immunosorbent assay (ELISA). The correlation between attachment loss (AL) and the levels of IL-18 and HIF-lα in hypoxia periodontitis group was evaluated.

RESULTSThe levels of IL-18 and HIF-lα in hypoxia periodontitis group were significantly higher than that in normoxia periodontitis and hypoxia control groups (P<0.05). Furthermore, the levels of IL-18 and HIF-lα in serum (r-0.792, r=0.753, P<0.05) and gingival tissues (r-0.817, r=0.779, P<0.05) were positively correlated with AL.

CONCLUSIONCIH could aggravate the destruction of periodontal tissues, which is correlated with IL-18 and HIF-lα levels.

Animals ; Disease Models, Animal ; Enzyme-Linked Immunosorbent Assay ; Gingiva ; Hypoxia ; Hypoxia-Inducible Factor 1, alpha Subunit ; Interleukin-18 ; Periodontal Index ; Periodontitis ; Rats ; Rats, Sprague-Dawley ; Serum ; Sleep Apnea, Obstructive

The quality of sleep has a great relationship with health and working efficiency. The result of sleep stage classification is an important indicator to measure the quality of sleep, and it is also an important way to diagnose and treat sleep disorders. In this paper, the method of detrended cross-correlation analysis (DCCA) was used to analyze sleep stage classification, sleep electroencephalograph signals, which were extracted from the MIT-BIH Polysomno graphic Database randomly. The results showed that the average DCCA exponent of the awake period is smaller than that of the first stage of non-rapid eye movement (NREM) sleeps. It is well concluded that the method of studying the sleep electroencephalograph with this method is of great significance to improve the quality of sleep, to diagnose and to treat sleep disorders.
Electroencephalography ; Humans ; Signal Processing, Computer-Assisted ; Sleep Stages

Microbial genome reduction and modification are important strategies for constructing cellular chassis used for synthetic biology. This article summarized the essential genes and the methods to identify them in microorganisms, compared various strategies for microbial genome reduction, and analyzed the characteristics of some microorganisms with the minimized genome. This review shows the important role of genome reduction in constructing cellular chassis.
Genes, Essential ; genetics ; Genetic Engineering ; Genome, Microbial ; genetics ; Synthetic Biology ; methods

The aim of establishment of animal models is to replicate human diseases in the animals and serve studies on causes, symptoms, pathogenesis, diagnosis and treatment, etc. of those diseases. Up to now, there is no ideal animal model of Alzheimer's disease(AD). The lag of animal models of Alzheimer's disease (AD) has greatly restricted the studies on of AD-related drug development. Regarding the causes and mechanisms of AD, some more desirable AD animal models have been developed. In recent years, some transgenic AD animal models have emerged and much facilitate the studies on etiology and pathogenesis of AD. However, this model can not replicate all the features of AD. The most striking distinction is a lack of neurofibrillary tangles (NFTs), and in some types of transgenic models (especially single transgenic models), no extensive neuronal loss is observed. Although tau protein has been detected in those models by immunohistochemical methods, paired helical filaments (PHF) have never been found. In this article, we are trying to review on some hot topics of development and application of transgenic mouse models in AD research.

Objective To study the distribution of purinergic P2X_3 receptor immunoreactive afferent fibers in the rat pharyngeal mucosa and provide morphological evidence for exploring the role of ATP in the signal transduction of sensory stimuli. Methods Twelve adult Wistar rats were used and immunofluorescenthistochemical double-labeling technique combined laser confocal scanning microscope was applied in the present study. Results 1.P2X_3 immunoreactive fibers were observed on sections from all parts of pharyngeal mucosa. Two types of positive fibers were found. One was free nerve fibers covered with many varicosities. Another one ramified in mucosa and showed complex arborization endings. Nerve plexus in mucosa were formed by P2X_3 immunoreactive fiber ramifications. 2.Most calcitonin gene-related peptide (CGRP) positive fibers intermingled with P2X_3 immunoreactive fiber arborizations. A few P2X_3 immunoreactive fibers were covered with many varicosities co-localized with CGRP. 3.In petrosal ganglion, most neurons were stained with P2X_3 or CGRP immunoreactivity and small number of P2X_3 immunoreactive neurons co-stained with CGRP. Conclusion These results indicated that the different types of afferent fibers in rat pharyngeal mucosa expressed purinergic P2X_3 receptor immunoreactivity and ATP might be related to nociceptive or physiological signals transduction in rat pharyngeal mucosa.

Triple-negative breast cancer is defined by the absence of expression of oestrogen, progesterone, and Her-2 receptors. The clinicopathological characteristics include: higher histological grades, earlier relapse with worse prognosis, and a higher incidence of visceral metastases than bone metastases. Since it has no response to hormone treatment and targeted therapy for Her-2, chemotherapy became the main therapy. In addition, it may have a relationship with basal-like breast cancer and BRCAl-related breast cancer on the phenotype and molecular level. Inter-individual variations in DNA damage and repair have been associated with an increased risk of breast cancer. BRCA1 together with its associated genes such as BRCA2, ATM, RAD51 and CHEK2 play vital roles in different DNA repair pathways to preserve genome stability. Therefore variations of BRCA1 and its associated genes in multiple repair pathways may result in reduced DNA repair capacity and altered sensitivity to cytotoxic chemotherapeutic agents,which could promote incidence of distant metastases. Reduced DNA repair capacity and dysfunctional BRCA1 pathway in triple-negative breast cancer have been reported, however the detailed mechanisms are still unknown. Do the genetic variations of tumor cells or resistance of tumor cells to therapeutic drugs play the dominant role in high incidence of metastases in patients with triple-negative breast cancer? In this review, the association of dysfunctional BRCA1-related DNA repair pathways with high incidence of metastasis in triple-negative breast cancer would be introduced.

ObjectiveTo observe the changing of the level of high-sensitivity C-reactive protein level and serum lipids in elderly patients with early chronic kidney disease. Methods90 cases of early chronic kidney disease were collected and 90 cases without chronic kidney disease served as control.The levels of total cholesterol(TC),triglyeride(TG),high density lipoprotein(HDL),low density lipoprotein(LDL)and high-sensitivity C-reactive protein (hs-CRP)were measured. ResultsThere was significant difference between early chronic kidney disease group and the control group on TG,HDL and hs-CRP(all P＜0.05).The level of HDL in study group was significantly lower than those in control group(P＜0.05＝. ConclusionThe patients with early chronic kidney disease often had hypertriglyceridemia,declining HDL and elevating hs-CRP.It should be paid attention early.

Objective To study the effect of different analgesia methods on postoperative cellular immune function in patients with radical resection of lung cancer.Methods Fifty patients undergoing selective radical resection of lung cancer were randomly divided into two groups.Group A (25 patients) was given morphine patient-controlled epidural analgesia(PCEA) and group B (25 patients) was given preemptive analgesia with parecoxib combined with morphine PCEA.T-lymphocyte subsets (CD4+,CD8+)and natural killer cell (NK cell) were measured by flow cytometry at preanesthesia,2 h intraoperative,24,48 h postoperative.Results The level of CD4+,CD8+ and NK cell in two groups at preanesthesia had no significant difference (P ＞0.05).The level of CD4+ and CD4+/CD8+ in two groups at 2 h intraoperative,24,48 h postoperative were significantly lower compared with preanesthesia (group A:CD4+:0.252 ± 0.035,0.227 ±0.025,0.248 ± 0.028 vs.0.310 ± 0.032,CD4+/CD8+:1.2 ± 0.1,1.0 ± 0.1,1.1 ± 0.2 vs.1.5 ± 0.2;group B:CD4+:0.265 ± 0.033,0.263 ± 0.028,0.264 ± 0.031 vs.0.312 ± 0.035,CD4+/CD8+:1.3 ± 0.2,1.2 ± 0.1,1.2 ± 0.1 vs.1.4 ± 0.1)(P ＜ 0.05).Compared with group A,the range of descend of CD4+ and CD4+/CD8+ in group B was significantly less at 24,48 h postoperative (P ＜ 0.05).The level of NK cell in two groups at 24,48 h postoperative was significantly lower than preanesthesia(O.112 + 0.019,0.113 + 0.016 vs.0.126 + 0.019 and 0.117 +0.016,0.120 +0.018 vs.0.127 + 0.021) (P ＜0.05).The level of NK cell in group B at 24,48 h postoperative was significantly higher than that in group A (P ＜ 0.05).Conclusion Parecoxib sodium preemptive analgesia combined with morphine PCEA may reduce immune depress in tumor palients by lowering the range of descend of CD4+ and NK cell.