One hundred and eighty patients of shoulder periarthritis were treated by combined traction, acupuncture, physiotherapy, Tuina and functional exercise. After 10 treatments, 133 cases were cured, 36 cases got marked effectiveness, 8 cases got effectiveness, 3 cases had no effectiveness and the total effective rate was 98.3%. Combined therapy can relieve adhesion of soft tissues induced by various causes to achieve the purpose of accelerating recovery.

Objective To observe the clinical therapeu tic effect of hyperkinetic syndrome of childhood treated by electroacupuncture plus acupoint application. Method Sixty-five cases with hyperkinetic syndrome of childhood were treated by electroacupuncture plus acupoint application (electroacupuncture group); 53 cases were treated by acupuncture (acupuncture group) and 53 cases were treated by Ritalin (west drug group). The above three groups were compared with each other in therapeutic effect. Results The effective rate of treating hyperkinetic syndrome of childhood by electroacupuncture plus acupoint application was 87.7%; the effective rate in west drug group was 86.8% and in acupuncture group was 77.4%. A comparison among the three groups showed there was no significant difference in clinical ther apeutic effect ( P > 0.05 ). Conclusion Electroacupuncture plus acupoint application was an effective therapy of hyperkinetic syndrome of childhood.

Objective To investigate the clinical characteristics of upper gastrointestinal diseases in very old people (aged ≥ 90 years),and to explore the necessity and safety of the gastroscopy for the super elderly people.Methods Clinical data of 50 very old people were retrospectively analyzed.Results The total detection rate of upper gastrointestinal disease was 76.0％ (38/50),in which the reflux esophagitis (RE) was the most common (26.0％),followed by erosive gastritis (20.0％),atrophic gastritis (18.0 ％),peptic ulcer (12.0 ％),gastrointestinal cancer (10.0％),duodenitis (8.0％) and fundic gland polyps (6.0％) respectively.RE lesions detected by gastroscopy were mainly mild and were divided into endoscopic grade LA-A (46.1％),LA-B (30.8％),LA C (7.7％),and LA-D (15.4％) according to the Los Angeles classification.The most common symptoms were abdominal distension and anorexia,and the both incidences were 12.0％.There was no significant difference in the prevalence of clinical symptoms between the elderly people with upper gastrointestinal disease and without abnormal gastroscopic findings [44.7％ (17/38) vs.41.7％ (5/12),x2 =0.035,P＞0.05].22 patients (44.0％) underwent gastroscopy under intravenous anesthesia.Conclusions The incidence of upper gastrointestinal disease is high in very old people,and mild reflux esophagitis is the most common.Clinical symptoms are little or asymptomatic in most very old people.Gastroscopy under intravenous anesthesia is safe and feasible for the very old people.

Purpose:To analyze retrospectively the clinical manifestation, treatment and prognosis. Methods:From 1978 to 1996,16 patients with olfactory neuroblastoma were treated.4 cases were stage A,6 cases were stage B and 6 cases were stage C. Six patients were treated with radiotherapy alone or combined with chemotherapy, 9, with surgery and postoperative radiotherapy and 1,with operative alone. The radiation dosage ranged from 40～70Gy with 2 Gy per day. Results:The 1 , 3 and 5 year survival rates were 75%, 75% and 69%.Conclusions:Olfactory neuroblastoma is sensitivity for radiotherapy, for the patients with stage A, radiotherapy alone is effective for stage B and C olfactory neuroblastoma , combined modality of surgery and postoperative radiotherapy should be the treatment of choice.

Objective　To apply high frequency ultrasound to laser treatment of children facial subcutaneous cavernous hemangiomas.Methods　Ten children with facial subcutaneous hemangiomas were treated with Nd:YAG laser, the process was supervised with high frequency ultrasound.Results　Before treatment,tumors showed low and inhomogenous echogenicity with blood flow signal;after treatment,the tumors appeared high and homogenous echogenicity with little or no blood flow signal. Conclusions　The high frequency ultrasound is helpful for laser treatment of facial subcutaneous cavernous hemangiomas in preoperative location and postoperative assessment.

Objective To investigate the disease causing gene of Stargardt disease. Method Fifteen patients with Stargardt disease were analyzed with 11 primers of the 11 exons of ABCR gene by using PCR SSCP and DNA direct sequencing techniques. Results Three newly detected disease causing mutations were found. Among those mutations, one is a frameshift mutation and others are single base transition. Conclusion This research confirmed that ABCR gene is associated with Stargardt disease, and 3 new mutations of ABCR gene were found.

Objective To observe the morphologic features of neurons labeled with green fluorescent protein (GFP) gene recombinant Sindibis virus in the deep part of lamina Ⅲ in the medullary dorsal horn (MDH) of the rat. Methods Neurons were infected and labeled with GFP gene recombinant Sindibis virus injected into lamina Ⅲ.Immunohistchemical staining showed the labeling results.The morphologic features of the GFP labeled neurons were revealed after reconstruction. Results A few GFP-labeled single neurons were found in the distal portions away from the virus injection site within the deep part of lamina Ⅲ of the MDH.According to the morphological features,especially their axons and axonal collaterals,GFP labeled neurons were classified into projection neurons and intrinsic neurons.The axons and their collaterals of the projection neurons entered to the superficial part of lamina Ⅲ,lamina Ⅱ and/or the medullary reticular formation.Most of the axons and their collaterals of the intrinsic neurons extended mainly within lamina Ⅲ.Conclusions According to the morphological features of axons and their axonal collaterals,GFP labeled neurons were classified into projection neurons and intrinsic neurons in the deep part of lamina Ⅲ of the MDH.GFP gene recombinant virus labeling technique is an effective method to investigate the morphological features of neurons.

Objective Comparison on the influence of invigorating spleen and restoring virility to the NPY level in brain and gene expression. Method Establishing the spleen insufficiency model of rat by adopting bitter decline diarrhea, wild eating and overworking, to examine the hypothalamus ventral kernel, the hippocampi CA1 area and forehead cortex, the gene expression change of NPY. Result In the model group, the immunoreaction masculine substance of NPY, hypothalamus ventral kernel,hippocampi CA1 area and prefrontal cortex decreases obviously. The immunoreaction masculine substance of NPY on above mentioned parts in the group of invigorating spleen and restoring virility increases distinctly. In the model group, the expression of NPY mRNA on prefronta cortex and hypothalamus ventral kernel decreases obviously, while on the groups of invigorating spleen and restoring virility increases obviously. Conclusion In the model group, the NPY level and gene expression in brain to promote study memory changed. Invigorating spleen and restoring virility may affect study memory by regulating the NPY level and gene expression.

Objective To study whether L-cysteine and lysolecithin (16:0) could be as a serum markers in the detection of OVC for overcoming the OVC defects of early detection .Methods Totally 142 cases of healthy check -up patients ( control group ) 100 cases from First Affiliated Hospital of Wenzhou Medical University in January 2012 to January 2014 were extracted patient specimens .These pa-tient specimens were used to detect by MALDI -TOF-MS mass spectrometer and obtain the peak m/z by the method of principal compo-nent analysis for screening expression difference between the two groups in the metabolites and the correlation between metabolites and pathological grade.Results The most difference substance between two groups were 184.05 and 496.30m/z which identified as LPC (16:0) and HCA;On HCA average level and detection rate , OVC group was significantly higher than control (P<0.01); On 184.05 and 496.30m/z peak area, control group was significantly higher than OVC group (P<0.01);HCA positively was correlated with patho-logic grade (P<0.05);184.05 and 496.30 m/z peak area were negative correlation with pathologic grade (P<0.05).Conclusion L-cysteine and lysolecithin (16:0) mechanism in the pathogenesis of OVC is unclear , but it can be used for the detection of serum OVC markers.

Objective To evaluate the pathophysiology of paraplegia during transarterial chemoembolization in liver cancer and investigate effective management and prevention for improving clinical situation and relieve major symptoms.Method 2758 patients accepted TACE procedure because of liver cancer(and/or combined with remote metastasis),demonstrated 4 cases suffering from paraplegia(3 males,1 female)since Mar.2003 to Feb.2005,with mean age of(51?14)years old,The operative records and the clinical features after chemoembolization were summarized in detail.Results The incidence of paraplegia was 0.145%,with major symptoms of dysesthesia and hypokinesise emerging within 4 hours after TACE,and most symptoms aggravating gradually within 24-48 hours untill paraplegia appeared,and then turned to stabilization about 5-7 days,with their body functions partial recovery in 2 months.Conclusion More attention should be paied to prevent ectopic embolization of spinal cord vessels originating from extrahepatic collateral arteries during TACE or TAE.