OBJECTIVE:To study the enterococcal distribution in clinical samples and their drug resistance to commonly use antibiotic and provide the guidance for rational administration.METHODS : Adopting paper diffusion method to observe the drug resistance of enterococci isolated from clinical samples in our hospital and analyzing their distribution in the samples.RESULTS :Enterococcus faecalis accounts for 44.1% in 227 strains and Enterococcus faecium accounts for 37.9%,the other kinds of enterococci were rare.There was no statistical difference between drug resistance of Enterococcus faecium and that of enterococcus faecalis. Vancomycin was the most potent antimicrobial.Penicillin,ampicillin,and nitrofurantion showed good antibacterial activity against enterococcus faecalis. Enterococcus faecium displayed high-level resistance against antibiotics in addition to glycopeptides.CONCLUSION:Doctor should pay attention to the drug resistance about clinically common pathogenic bacterium. Continual surveillance of resistance rate of enterococcus is important to direct the treatment of enterococcus infections.

Biomarkers ; Cystatin C ; blood ; Early Diagnosis ; Hepatorenal Syndrome ; diagnosis ; Humans ; Renal Artery

Anterior mandibular fracture, which includes symphyseal and parasymphyseal mandibular fractures, is one of the most common types of fracture in clinical work, and is usually associated with condyle fracture. This type of fracture predisposes the patients to facial widening, malocclusion, and ankylosis when not properly and timely treated because of the influence of its anatomic structure. This article reviews the characteristics, complication, diagnosis, and therapy of this type of fracture.
Humans ; Malocclusion ; Mandibular Condyle ; Mandibular Fractures

Objective:To discuss the management and maintenance of medical equipment in hospital, the department of biomedical and engineering should develop a reasonable management plan to meet the needs of using safety management of medical equipment based on the risk analysis.Methods: Make up a strategy based on the analysis of application risk according to the clinical function, physical risk, fault avoidable probability and the adverse events recorded, and conduct the preventative maintenance and safety performance inspection. Results: The focus of clinical engineering professionals change from passive acceptance to repair equipment to preventive equipment maintenance, and carry out safety performance inspection to reduce the risk caused by the failure of equipment which is harmful to the patients.Conclusion: The clinical engineering professionals need to pay attention to the application risk and improve the management strategy while they improve the technical level.

0.05).However,there were significant differences between the groups in HAMD score at the first weekend and second weekend(P

OBJECTIVE: To construct hospital pharmaceutical website and facilitate the evolvement of hospital pharmaceutical care.METHODS: Website management system was developed independently and the system was used to construct hospital pharmaceutical website in our hospital.RESULTS & CONCLUSIONS: Website management system which was designed with B/S three-tier structure which sustains multi-level management authority control and a variety of database and runs stably.The professional website based on website management system is convininent and maintenance,rapid in operation,cheap in cost,low in consumption of human resouces,comprehensive in function,highly professional,and it has greated enhanced the working efficiency in hospital pharmacy.

OBJECTIVE:To establish an RP-HPLC method for determination of the content of bortezomib in its crude drug. METHODS: Bortezomib was separated on Phenomenex-C18 chromatographic column with mobile phase consisted of methanol-phosphate buffer (pH 2.0) (60∶40) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 254 nm. RESULTS: The linear range of bortezomib was 50.72～152.16 ?g (r=0.999 9) with an average recovery rate of 99.62% (RSD=0.39%,n=6). CONCLUSIONS: The method is sensitive,specific and simple in operation,and it is applicable for the determination of bortezomib in its crude drug.

OBJECTIVE:To provide the reference for the set-up of precautions against flu pandemic in China.METHO_DS:RNA of influenza viruses isolated from flu children in flu seasons between 2003 and 2005 in Beijing was extracted and amplification of M2 gene fragments associated with ion channel were performed by one step reverse transcription–polymerase chain reaction(RT-PCR).The sequencing of amino acid was determined,and its bioinformation was analyzed with software,then the positions of amino acid for resistance to amantadine were detected.RESULTS:20 of 31 isolated strains were resistant to amantadine,the percentage of which was 66.7%.CONCLUSION:The monitoring of drug resistance to flu viruses should be strengthened in China.

Objective To explore the values of combined detection of carcino embryonic antigen (CEA),carcinoma antigen 125 (CA 125),cytokerain 19 fragment 21-1 (CYFRA2 1-1),carcinoma antigen Scc-Ag (Scc-Ag) and neuron specific enolase (NSE) for diagnosis and treatment of lung cancer.Methods The concentrations of CEA,CA125,CYFRA21-1,Scc-Ag and NSE in 198 patients with lung cancer (lung cancer group),50 with benign pulmonary disease (benign disease group) and 50 healthies (health control group) were detected by electrochemiluminescence immunoassay (ECLIA).The concentration of Pro-GRP31-98 was detected by enzyme linked immunoassay.Results Six tumor markers in lung cancer group showed significant diffcrence compared with those of benign disease group and health control group (P ＜ 0.01).The concentrations of serum tumor molecular markers were significantly higher in Ⅲ,Ⅳ stage group than those in [,Ⅱ stage group.Before the treatment,the concentrations of serum tumor molecular markers in lymph node metastasis group and lymph node recurrence group were significantly higher than those in no lymph node metastasis group and no lymph node recurrence group after the treatment (P ＜ 0.01).The positive rate of CEA,CA125 in lung adenocarcinoma group,CYFRA21-1 and Scc-Ag in lung squamous cell carcinomas group and NSE and Pro-GRP31-98 in small cell lung carcinoma group were significantly different from the other two kinds of pathological types (P ＜ 0.01).The positive rates of CEA and CA125 combinated detection in lung adenocareinoma,CYFRA21-1 and Scc-Ag combinated detection in lung squamous cell carcinomas,NSE and Pro-GRP31-98 combinated detection in small cell lung carcinoma was significantly higher than the individual detection respectively.The sensitivitv rates of CEA,CA125,CYFRA21-1,Scc-Ag,NSE and Pro-GRP31-98 were 52.52 ％,51.01％,48.99 ％,53.03 ％,49.49 ％ and 50.50 ％,respectively.And the combinated detection sensitivity rate of the six tumor markers was 96.46 ％,which significantly higher than that of a single marker (P ＜ 0.01).Conelusions The tumor molecular markers of CEA,CA125,CYFRA21-1,Scc-Ag,NSE and Pro-GRP31-98 may be the indicators in diagnosis of lung cancer.The combined detection of serum tumor molecular markers would significantly improve the sensitivity of diagnosis for lung cancers.At the same time,it should be valuablc for determination of clinical stage,histological type and follow-up studies of lung cancer.

Objective To investigate the methods and clinical outcome of the operative treatment of thoracolumbar fractures with pedicle screw single segment fixation and the approach through paravertebral muscles.Methods One hundred and twenty patients were selected as our subjects,who were suffered from thoracolumbar fracture without neurological deficit and underwent surgical treatment in the Central Hospital of Zibo from Jan.2009 to Dec.2010.All cases were randomly divided into mini-invasion group and control group.Patients in mini-invasion group were received the single segment fixation of the spatium intermusculare of longissimus muscle and multifidus,and in control group were received the traditional approach with short segment fixation group.The muscle gap approach along the longest muscle and multifidus muscle gap enters,preserving the integrity of the paraspinal muscles.Results The operation periods of mini-invasion group and control group were (53.8 ± 12.3) min and (88.5 ± 18.5) min (t =1.731,P ＜ 0.05),the intraoperative bleeding volume were (95.7 ±21.5) ml and (280.3 ±83.1) ml(t =2.414,P ＜0.05),the postoperative drainage volume were (20.4±8.2) ml and (132.9 ±50.3) ml(t =2.068,P ＜0.05) and inpatient time were (13.8 ±2.2)d and (20.4 ±5.5)d(t =1.853,P ＜0.05).However,there was no significant difference in terms of fracture healing time,visual analogue scale,Oswestry disability index and radiology(P ＞0.05).All cases had union of fractures and no reduction loss and fixation failure occurred.Condusion The treatment of pedicle screw single segment fixation and the approach through paravertebral muscles on thoracolumbar fracture is proved to be an effective method with minimally invasive,less bleeding volume,simple procedure and fast recovery method.