Objective To investigate the clinical efficacy of acupuncture in treating subhealth.Methods One hundred and fifty-four patients with subhealth were allocated, using a random number table, to observation and control groups, 77 cases each. The control group received basic treatment by diet, exercise, rest and rest and psychological intervention and the observation group, acupuncture therapy in addition. Subhealth scale subscores and the 36-Item Short-Form Health Survey (SF-36) subscore were recorded and blood biochemical indicators were measured in the two groups before and after treatment. the clinical therapeutic effects were compared between the two groups.Results The total efficacy rate was 96.1% in the observation group and 83.1% in the control group; there was a statistically significant difference between the two groups (P<0.05). There were statistically significant pre-/post-treatment differences in subhealth scale subscores in the two groups (P<0.05). After treatment, sleep, digestion, urination, positive emotion, ability and social relation subscores were higher, and fatigue, discomfort and negative emotion subscores were lower in the observation group than in the control group (both P<0.05). There were statistically significant pre-/post-treatment differences in blood biochemical indicators in the two groups (P<0.05). After treatment, blood uric acid (UA), total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) were significantly lower and high-density lipoprotein cholesterol (HDL-C) was significantly higher in the observation group than in the control group (both P<0.05). The 36-Item Short-Form Health Survey (SF-36) subscore were higher in thetwo groups after treatment than before treatment (P<0.05). After treatment, physiological function, role-physical, role-emotional, body pain and general health subscore were significantly higher in the observation group than in the control group (P<0.05).Conclusions Acupuncture helps to improve subhealth status in population, regulate blood biochemical indicators and enhance the clinical therapeutic effect to raise the quality of life in the patients.

Objective:To explore the correlation between fibrinogen levels and chemotherapy effect of platinum drugs in ovarian cancer patients. Methods:Totally 91 ovarian cancer patients and 95 patients with benign ovarian tumor were involved in our retrospec-tive study, and the associations between preoperative fibrinogen levels and clinic pathologic parameters in the patients were analyzed. Results:High fibrinogen levels were observed in 36.26％ (33/91) of the patients with ovarian cancer ( >4.0 g·L-1), which was significantly higher than that in the patients with benign tumor(P<0. 05). The high fibrinogen levels in ovarian cancer patients were associated with the higher FIGO stage and poor chemo-response, while were not associated with the patients' age, CA-125 levels and histological type and grade (P>0. 05). More importantly, no correlation was observed between high blood fibrinogen and CA-125 lev-els (P>0.05). Chemotherapy resistance was more in the patients with high fibrinogen levels than that in the other patients(P <0. 05). The OR value of high fibrinogen levels was 3. 571, 95％ CI was 1. 276-10. 000, which suggested high fibrinogen level is a dangerous factor for chemotherapy resistance. Conclusion:The study suggested that elevated fibrinogen level was a dangerous factor for chemotherapy response, which has a potential role in the poor prognosis of ovarian cancer patients independent of CA-125.

Objective To explore the effect of treadmill training on expression of glial fibrillary acidic protein (GFAP) and brain-derived neurotrophic factor (BDNF) after cerebral ischemia-reperfusion in rats. Methods 30 male Wistar rats were randomly divided into sham group, model group and treadmill training group, with 10 rats in each group. The latter 2 groups were modeled with middle cerebral artery occlusion for 2 hours and reperfusion. The treadmill training group underwent treadmill exercise on the 3rd day after modeling for 12 days. Neurological function was evaluated with modified Neurological Severity Scores (mNSS). The neuronal pathological change in ischemic cortex was observed with HE staining. The expressions of GFAP and BDNF in cortex were determined by Western blotting. Results Com-pared with the model group, the mNSS scores decreased in the treadmill training group (F=9.931, P<0.01), the pathological damage in the ischemia cortex significantly lessened, and the expressions of GFAP (t=6.73) and BDNF (t=3.78) increased (P<0.05). Conclusion Treadmill training may increase the expression of GFAP and BDNF after cerebral ischemia-reperfusion in rats, and promote the recovery of neurologi-cal function.

Objective:To explore the relationship between the latent classes of online emotion regulation among college students and the distribution difference of different types on variables such as gender,grade,ages of surfing the net and so on.Methods:Totally 421 college students were assessed with Online Emotion Regulation Questionnaire (OERQ).Latent class analysis was used to identify online emotion regulation classes,and then analyzed the distribution of latent classes among different genders,grades and ages of surfing the net.Results:Two latent classes of online emotion regulation were identified,including online emotion inhibiting/ignoring class (37.6％) and online emotion venting class (62.4％).The prevalence of online emotion venting class among girls (56.6％ vs.3.8％),sophomores (36.3％ vs.11.9％),juniors (23.4％ vs.3.1％),those with more than 36 months of surfing the net (31.6％ vs.18.8％) were significantly higher than the other of the respective kind.Whereas,the rate of inhibiting/ignoring class among boys (32％ vs.7.6％),freshmen (20％ vs.5％) were significantly higher.Conclusion:It suggests that online emotion regulation could be divided into two different latent classes and prevalence of each class is significantly different in different genders,grades,and ages of surfing the net.

Objective:To construct and identify recombinant Bb-MPT64 vaccine of mycobacterium tuberculosis. Methods: MPT64 antigen gene were amplified by PCR from mycobacterium tuberculosis H37Rv,then the gene was cloned into Escherichia coli-Bifidobacteria shuttle plasmid pGEX-1?T to construct pGEX-MPT64.The recombinant plasmid was electroporated into Bifidobacteria bifidum(Bb) to construct rBb-MPT64 vaccine.The expression of pGEX-MPT64 was induced with isopropyl-?-D- thiogalactopyranosid(IPTG) and protein MPT64/GST was examined by SDS-PAGE and Western blotting techniques. Results:688bp MPT64 gene was successfully amplified by PCR and cloned into pGEX-1?T by restriction endonuclease analysis.rBb-MPT64 vaccine was successfully constructed by PCR.SDS-PAGE and Western blotting demonstrated that the protein MPT64/GST were expressed in E.coli,BL21. Conclusion:rBb-MPT64 vaccine of mycobacterium tuberculosis is successfully constructed,which may provide basis for the development of new anti-tuberculosis vaccine.

Objectives:To determine the relationship between hyperuricemia and lipid meterbolism abnomaltity, hypertention, and weight index. Methods:Statistical analysis was given to 9 528 cases for their health examination results, including the blood uric acid,blood fat(total cholestrrol,trigly ceride,high density lipoprotein,low density lipoprotein), blood pressure,and BMI. Results:The detection rate of hyperuricemia was 18.13%(1 727cases),in which male were accounted for 13.44%,female 6.25%. In the 1 727 cases of hyperuicemia,41.31%(535cases) were detected with lipid metabolism,overweight 45.33%(587cases), obesity15.06%(195cases). Conclution:In the cases of hyperuricemia,there are more males than females,and the number of lipid metorbolism abnormality,hypertention,overweight, and obesity is higher than that in the normal groups. Reducing Weight,controlling hyperlipemia and keeping the normal blood pressure are the considerate measures for the prevention and care of hyperuricemia.

Objective:To compare the effect of histone deacetylase inhititor trichostatin A(TSA) on the proliferation and apotosis of different hepatoma cell lines and to investigate the effect of TSA on hepatitis B virus(HBV) replication. Methods:Human hepatoma cell lines HepG2,HepG2.2.15,QGY7701 and normal liver cell line L02 were treated with TSA, the effects on proliferation and apoptosis were examined by MTT assay and agarose gel electrophoresis of DNA; The viral proteins of hepatitis B surface antigen(HBsAg)and e antigen(HBeAg) in culture medium were measured using the Abbott Microparticle Enzyme Immunoassay kits;The medium HBV DNA levels were quantified using HBV real-time PCR kit. Results:(a)TSA treatment repressed proliferation and induced apoptosis in hepatoma cell lines;(b)The normal repressed proliferation and induced apoptosis in hepatoma cell lines;(b) The normal liver cell line L02 was not sensitive to the treatment of TSA;(c)TSA stimulated HBV replication. Conclusions:TSA treatment could inhibit proliferation of different hepatoma lines,and the normal liver cell line L02 is not sensitive to TSA treatment. However,TSA stimulates HBV replication. Therefore,our results suggest that TSA might be a good chemotherapic candidate for hepatocellular carcinoma patients without HBV infection but may not be suited to treat hepatocellular cancer patients with HBV infection.

Objective:To investigate the biologic reference interval and clinical meaning of laboratory indexs on intrahepatic cholestasis of pregnancy. Methods:506 normal pregnancy women and 358 intrahepatic cholestasis of pregnancy women(ICP) was elected. detected the laboratory indexs of intrahepatic cholestasis of pregnancy:TBA,TB,DB,CG respectively,and 506 normal pregnancy women based on gestational age ware compared,358 ICP women based on the serious degree of the condition ware compared. Results:the content of TBA,TB,DB,CG wasn't related to gestational age in 506 normal control group,and the biologic reference interval are: TBA,TB,DB,CG.There are obviously difference of the content of TBA、TB、DB、CG between the ICP women and the normal control group(P

The incidence of gastrointestinal diseases increases every year.Etiology and pathogenesis of gastrointestinal diseases have been not yet elucidated,such as inflammatory bowel disease,gastrointestinal cancers,and allergic diseases.In recent years,the role of mucin genes expression in gastrointestinal diseases has been paying much attention to.Studies have shown that mucins are the main component of mucus in the digestive tract,and a major factor in the formation of the gastrointestinal barrier.Mucins are related to gastrointestinal epithelium,intestinal microflora and inflammatory factors.Abnormal expression of gastrointestinal mucin genes,especially MUC1 and MUC2,are related to Gastrointestinal inflammation and cancer.Currently gastrointestinal mucin genes expression contribute to exploring the diagnosis,treatment and prognosis of digestive diseases.This study is to summarize the progress in gastrointestinal mucin genes expression.

A method was developed for the determination of ochratoxin A (OTA) in human urine by HPLC-FLD after molecularly imprinted polymer solid phase extraction (MIP-SPE) column. After the pH being adjusted to 2.5 with 0.1 mol x L(-1) HC1, sample was cleaned up with MIP-SPE column for ochratoxin A, the analyte was analyzed by high performance liquid chromatography coupled with fluorescence detection (HPLC-FLD), and finally all the positive results were confirmed by LC-MS/MS. Recoveries from urine samples spiked with OTA at levels ranging from 2 to 20 ng x mL(-1) were 90.6%-101.9%, and RSDs were 0.1%-1.6%. Sixty-five volunteers living in Beijing took part in the study, of which 5 were found containing OTA in their urine and the highest value was 0.091 ng x mL(-1). The MIP-SPE column was firstly applied to purify and concentrate OTA in human urine, this method is simple, rapid and reliable and can be used to determine the contents of OTA in human urine.