Objective To explore the effect of treadmill training on expression of glial fibrillary acidic protein (GFAP) and brain-derived neurotrophic factor (BDNF) after cerebral ischemia-reperfusion in rats. Methods 30 male Wistar rats were randomly divided into sham group, model group and treadmill training group, with 10 rats in each group. The latter 2 groups were modeled with middle cerebral artery occlusion for 2 hours and reperfusion. The treadmill training group underwent treadmill exercise on the 3rd day after modeling for 12 days. Neurological function was evaluated with modified Neurological Severity Scores (mNSS). The neuronal pathological change in ischemic cortex was observed with HE staining. The expressions of GFAP and BDNF in cortex were determined by Western blotting. Results Com-pared with the model group, the mNSS scores decreased in the treadmill training group (F=9.931, P<0.01), the pathological damage in the ischemia cortex significantly lessened, and the expressions of GFAP (t=6.73) and BDNF (t=3.78) increased (P<0.05). Conclusion Treadmill training may increase the expression of GFAP and BDNF after cerebral ischemia-reperfusion in rats, and promote the recovery of neurologi-cal function.

Objective:To explore the relationship between the latent classes of online emotion regulation among college students and the distribution difference of different types on variables such as gender,grade,ages of surfing the net and so on.Methods:Totally 421 college students were assessed with Online Emotion Regulation Questionnaire (OERQ).Latent class analysis was used to identify online emotion regulation classes,and then analyzed the distribution of latent classes among different genders,grades and ages of surfing the net.Results:Two latent classes of online emotion regulation were identified,including online emotion inhibiting/ignoring class (37.6％) and online emotion venting class (62.4％).The prevalence of online emotion venting class among girls (56.6％ vs.3.8％),sophomores (36.3％ vs.11.9％),juniors (23.4％ vs.3.1％),those with more than 36 months of surfing the net (31.6％ vs.18.8％) were significantly higher than the other of the respective kind.Whereas,the rate of inhibiting/ignoring class among boys (32％ vs.7.6％),freshmen (20％ vs.5％) were significantly higher.Conclusion:It suggests that online emotion regulation could be divided into two different latent classes and prevalence of each class is significantly different in different genders,grades,and ages of surfing the net.

Objective To investigate the clinical efficacy of acupuncture in treating subhealth.Methods One hundred and fifty-four patients with subhealth were allocated, using a random number table, to observation and control groups, 77 cases each. The control group received basic treatment by diet, exercise, rest and rest and psychological intervention and the observation group, acupuncture therapy in addition. Subhealth scale subscores and the 36-Item Short-Form Health Survey (SF-36) subscore were recorded and blood biochemical indicators were measured in the two groups before and after treatment. the clinical therapeutic effects were compared between the two groups.Results The total efficacy rate was 96.1% in the observation group and 83.1% in the control group; there was a statistically significant difference between the two groups (P<0.05). There were statistically significant pre-/post-treatment differences in subhealth scale subscores in the two groups (P<0.05). After treatment, sleep, digestion, urination, positive emotion, ability and social relation subscores were higher, and fatigue, discomfort and negative emotion subscores were lower in the observation group than in the control group (both P<0.05). There were statistically significant pre-/post-treatment differences in blood biochemical indicators in the two groups (P<0.05). After treatment, blood uric acid (UA), total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) were significantly lower and high-density lipoprotein cholesterol (HDL-C) was significantly higher in the observation group than in the control group (both P<0.05). The 36-Item Short-Form Health Survey (SF-36) subscore were higher in thetwo groups after treatment than before treatment (P<0.05). After treatment, physiological function, role-physical, role-emotional, body pain and general health subscore were significantly higher in the observation group than in the control group (P<0.05).Conclusions Acupuncture helps to improve subhealth status in population, regulate blood biochemical indicators and enhance the clinical therapeutic effect to raise the quality of life in the patients.

Objective:To explore the correlation between fibrinogen levels and chemotherapy effect of platinum drugs in ovarian cancer patients. Methods:Totally 91 ovarian cancer patients and 95 patients with benign ovarian tumor were involved in our retrospec-tive study, and the associations between preoperative fibrinogen levels and clinic pathologic parameters in the patients were analyzed. Results:High fibrinogen levels were observed in 36.26％ (33/91) of the patients with ovarian cancer ( >4.0 g·L-1), which was significantly higher than that in the patients with benign tumor(P<0. 05). The high fibrinogen levels in ovarian cancer patients were associated with the higher FIGO stage and poor chemo-response, while were not associated with the patients' age, CA-125 levels and histological type and grade (P>0. 05). More importantly, no correlation was observed between high blood fibrinogen and CA-125 lev-els (P>0.05). Chemotherapy resistance was more in the patients with high fibrinogen levels than that in the other patients(P <0. 05). The OR value of high fibrinogen levels was 3. 571, 95％ CI was 1. 276-10. 000, which suggested high fibrinogen level is a dangerous factor for chemotherapy resistance. Conclusion:The study suggested that elevated fibrinogen level was a dangerous factor for chemotherapy response, which has a potential role in the poor prognosis of ovarian cancer patients independent of CA-125.

Objective To explore the effects of low-concentration heparin sodium (LCHS) on preventing thrombophlebitis in patients with peripherally inserted central catheter (PICC). Methods Totally 287 patients who received PICC from 2007 to 2009 in our department were divided into LCHS group ( n = 128 ), in which the patients had no coagulation disorders or other blood diseases and the used drugs were compatible with heparin sodium, and control group ( n = 159). In the LCHS group, LCHS ( 10 U/ml) was used for irrigation and sealing of the catheter with positive pressure. In the control group, 0. 9% normal saline was used for the same procedures. Changes of the clinical manifestations of phlebitis and the values of plasma fibrinogen in both two groups were observed.Results In LCHS group, 21 patients had obvious clinical manifestations of phlebitis, among whom 8 patients met the diagnostic criteria of thrombophlebitis. On the contrary, in control group, 38 patients had obvious clinical manifestations of phlebitis ( P ＜ 0. 05 ), among whom 31 patients met the diagnostic criteria of thrombophlebitis ( P ＜0. 001 ). The changes of plasma fibrinogen were also significantly different between these two groups ( P ＜ 0. 001 ).Conclusion LCHS can prevent thrombophlebitis in patients with PICC who have no coagulation disorder or other blood diseases and the used drugs are compatible with heparin sodium.

The incidence of gastrointestinal diseases increases every year.Etiology and pathogenesis of gastrointestinal diseases have been not yet elucidated,such as inflammatory bowel disease,gastrointestinal cancers,and allergic diseases.In recent years,the role of mucin genes expression in gastrointestinal diseases has been paying much attention to.Studies have shown that mucins are the main component of mucus in the digestive tract,and a major factor in the formation of the gastrointestinal barrier.Mucins are related to gastrointestinal epithelium,intestinal microflora and inflammatory factors.Abnormal expression of gastrointestinal mucin genes,especially MUC1 and MUC2,are related to Gastrointestinal inflammation and cancer.Currently gastrointestinal mucin genes expression contribute to exploring the diagnosis,treatment and prognosis of digestive diseases.This study is to summarize the progress in gastrointestinal mucin genes expression.

Food allergy is the earliest and most common allergic disease in infants that affects children' s growth and development.It is closely related to other allergic diseases with a great increase in prevalence.It is important to learn the mechanisms and related factors,investigate the prevention and treatment,and explore the influence to other allergic diseases with food allergy in infants.

ObjectiveTo analyse the prognostic factors of acute respiratory distress syndrome (ARDS) of children by comparing mortality in ARDS. MethodARDS type ,treatment process if there were multiple organ dysfunction syndrome (MODS), and whether family members actively cooperated with the treatment factors in 38 children with ARDS were analyzed. ResultsARDS of the original mortality of sepsis was the highest[ 66.67％ ( 14/21 )]; treatment process combined with MODS in 8 cases, 6 died, the mortality up to 75.00％ (6/8), significantly higher than combined without MODS [40.00％ (12/30)],there was significant difference (P ＜ 0.05); family members actively cooperated with the treatment could reduce mortality of children (P ＜0.05); PaO2 and PaO2/FiO2 value:pulmonary-exterior type was lower than pulmonary-interior type, combined with MODS was lower than combined without MODS, family members actively cooperated with the treatment was lower than family members inactively cooperated with the treatment (P ＜ 0.05 );PaCO2 value:pulmonary- exterior type was lower than pulmonary-interior type, family members actively cooperated with the treatment was lower than family members inactively cooperated with the treatment (P ＜ 0.05 ) ; pH: pulmonary-exterior type was higher than pulmonary-interior type, combined with MODS was higher than combined without MODS, family members actively cooperated with the treatment was higher than family members inactively cooperated with the treatment (P ＜0.05);the critical illness score in family members with a positive attitude was higher than that in family members with a negative attitude (P ＜ 0.05).ConclusionThe primary disease of sepsis, associated with MODS, the family members with a negative attitude toward the treatment affect the prognosis of children with ARDS.

ObjectiveTo investigate the clinical manifestation and therapy method of hyperthyroidism accompanied by liver damage. MethodsTo analyze the clinical data of 38 cases those with hyperthyroidism accompanied by liver damage and divide them by damage degree into two groups,one of which contains mild impairment of 26 cases and the other one contains severe impairment of 12 cases. Then make a analysis about the comparison of the two groups on the level of thyroid disorders and the correlation with liver damage. ResultsOf all the 38 cases 26 (68. 4％ ) cases have slight liver damage and 12(31.6％ ) cases have severe liver damage. The symptom:26 cases have abdominal distension in different degree, weakness, sickness, port colored urine, aurigo and so on. 2 cases have hepatomegaly. 10 cases have no obvious symptoms of liver damage. The FT3 and FT4 in the severely liver-impaired group is obviously higher than the slightly liver-damaged group( (t =3.16,3.27, allP ＜ 0. 05 ) ,while the TSH is obviously lower( t =3.09 ,P ＜ 0.05 ). 38 cases are all healed and the symptom of hyperthyroidism has got under control. The cases of ATD therapy or 131 I therapy are not any aggravated in liver damage. ConclusionHyperthyroidism may lead to liver damage whose level is directly proportional to the level of thyroid dysfunction. And the result can be different, even no any symptom.

Objective To observe the effect of comfort care on psychological status and life quality of patients undergoing interventional treatment of coronary heart disease. Methods 86 cases patients with coronary heart disease were randomly classified into the observation group and the control group with 43 cases in each group. The observation group was treated with comfort care and the control group was treated with normal nursing. The psychological status and life quality between the two groups were compared. Results Compared with pre-nursing, not only physiological function, independence, spiritual beliefs, social relations and quality of life but also SAS and SDS in the observation group were significantly improved after nursing. However, there were no significant differences in the control group. Moreover, the scores of life quality and anxiety and depression were significantly better than those in the control group. Conclusions Comfort care on patients with coronary heart disease can improve anxiety and depression status, servicing satisfaction and life quality of patients.