Objective To study the reasons why PLoS One has set off an upsurge in China especially in hospitals, the academic level of papers published in PLoS One, and how to correctly judge the SCI-covered papers published by hospitals.Methods Papers published in PLoS One by hospitals in China were retrieved.The number of papers published in PLoS One by hospitals in China, the institutions that published the papers in PLoS One, and the num-ber of fund-supported papers were analyzed.Results A total of 9739 papers were published in PLoS One by hospitals in China from 2007 to October 2015, with an average cited frequency of 5.29.The majority of authors who published the papers were from affiliated hospitals of scientific research institutions and teaching hospitals of colleges and uni-versities.Of the 9739 papers, 87.78%were supported by funds from different foundations.Conclusion Nothing is strike about the academic level of papers published in PLoS One by hospitals in China with their average cited fre-quency lower than that (8.14) in our country.The SCI-covered papers published by hospitals should not be con-sidered as the only criterion in assessing the performances of staff in hospitals.

目的观察急性心肌梗死患者早期康复护理的远期效果。方法40例老年急性心肌梗死患者随机分为实验组20例(早期康复组)和对照组20例(常规治疗组),前者接受2周的康复护理,后者接受3周的康复护理。对两组患者进行为期18个月的随访,观察其心功能、活动情况,及心绞痛和再梗死的发生率。结果两组患者的心功能无明显差异,但实验组患者心绞痛的次数和再梗死的发生率低于对照组(P<0.05)。结论对急性心肌梗死患者进行早期康复是安全、可行的,可改善心功能和降低再梗死的发生率,减少并发症的发生。

Female ; Humans ; Melanosis ; Middle Aged ; Occupational Diseases

Objective To investigate the distribution and the characteristics of drug resista-nce of coagulase negative Staphylococcus,and to provide the reference for properly choosi-ng antibiotic therapy of coagulase negative Staphylococcal infecs.Methods Contrasted 484 coagulase negative Staphylococcus(CNS) species isolated in hospital from 2001 to 2003 and 512 species from 2004 to 2007.Nosocomial CNS was identified and then drug resistance test was performed by K-B method,Nitrocefin method was utilized to detect β-lactamase.Clinical and Laboratory Standards Institute.Perfor-mance Standards forantimicro-bial susceptibility testing;Fifteenth information supplement.CLSI/NCCLS document M100-S17[J].Clinical and Laboratory Standards.Results The drug-resistant rate of.CNS to penicillin,erythromycin,oxacillin,amoxicillin/clavulanate,clindamycin,cepzolin,ceftriaxone and cefepime was high(>70%),whereas that to rifampin,nitrofurantoin,amikacin,tetracycline and trimetholvrim/sulfamethoxazole was low(<50%).No drug-resistant CNS strains to vancomyein were found.Conclusions,and no outstanding imparity from two groups.Conclusion The patterfi of resistance has changed greatly in the past 3 years.Clinical physicians should pay attention to properly choosing and using antibiotics.

Objective To investigate the clinical value ofcombined detection of the serum levels of CA125,TSGF and DD in the diagnosis of ovarian cancer. MethodsThe serum CA125 and DD were determined by enzyme linked immunosorbent assay and TSGF was determined by biochemistry shade selection in all 252 patients(including 84 cases of ovarian cancer,84 cases of ovarian benign diseases,84 cases of controls). ResultsThe concentration of CA125,TSGF and DD in the diagnosis of ovarian cancer group were higher than those in ovarian benign diseases group and the controls(all P＜0.05).There was no statistical significance in the concentration of CA 125,TSGF and DD between ovarian benign diseases group and the controls(all P ＞ 0.05).In combined assay of CA 125,TSGF and DD,the sensitivity rate was 94.05%,the specificity rate was 90.48%,and the accuracy rate was 92.26%.Comparing with the single assay,combined analysis had much higher diagnostic sensitivity(all P＜0.05). ConclusionThe assay of CA125,TSGF and DD had significant dinical value in diagnosis of breast cancer,combined detection could increase the positive detection of ovarian cancer,and compensate the use of only one tumor marker.

Crocin is a chemical extraction from saffron and it is the most important kind of pigment of saffron.Recently,the research on cervical cancer,bladder cancer,leukemia,tongue squamous cell carcinoma,colon cancer,pancreatic cancer shows that crocin has a role in inducing apoptosis of the tumor cells by activating caspase-3,regulating the ratio of bcl-2/bax and repressing the expression of survivin.Crocin has a strong inhibitory effect on tumor proliferation by inducing cell cycle to arrest through down-regulating the expression of cyclin D1 and up-regulating the expression of p27kip1.Crocin can also exert antitumor effects by liposome encapsulation to enhance cytotoxic effect.Crocin is a kind of effective chemical for tumor treatment and prevention with no significant toxico-and by-effect in vivo,and is likely to be used as an anticancer drug clinically in the future.

Pediatric liver transplantation has long been developed in the western world.Currently,favorable outcomes after liver transplantation have been achieved in pediatric recipients and the postoperative 5-year survival rate reached 80％ in western transplantation centers.In the mainland China,pediatric liver transplantation started quite recently and there is still a big gap compared with western centers.In addition,there is unbalanced development between adult and pediatric recipients due to surgical difficulties and undesirable outcomes following pediatric liver transplantation.The operation methods of pediatric liver transplantation include whole liver transplantation,reduced-size liver transplantation,living donor liver transplantation and split liver transplantation.Perioperative complications of pediatric liver transplantation mainly include portal vein thrombosis,hepatic artery thrombosis,intra-abdominal bleeding,infections,rejection,bile leakage and biliary strictures.While the long-term complications are infections,post-transplantation lymphoproliferative disorders,acute and chronic rejections caused by poor compliance with immunosuppressive therapy.Perioperative complications have been reduced recently thanks to improvement of surgical techniques.With the highly-prolonged survival period,long-term postoperative complications have been playing a significant role in recipients' survival rates.Management of long-term follow-up and compliance has been the next focus of pediatric liver transplantation.

Pancreatic cancer is one of the most malignant gastrointestinal tumors,characterized by a poor prognosis,absence of specific clinical symptoms and signs,prone to metastases and lack of response to conventional therapy.1 year survival rate of this disease increased to about 20 ％ by gemcitabine,but the morbidity and mortality rates are still higher and higher in the whole worldwide.DNA methylation is one of the mechanisms of epigenetics which is the study of heritable changes in gene expression that are not coded in the DNA sequence itself.It seems that DNA methylation indeed plays an important role in the development and progression of pancreatic cancer.

Newborns,especially premature newborns,because of immature of immune function,have high incidence of sepsis.Today sepsis is still an important cause of neonatal death and poor prognosis.Unfortunately,clinical signs of sepsis are non-specific,so early diagnosis and treatment of the newborn infant with suspected sepsis are essential to prevent severe complications.Blood culture is the gold standard for diagnosis of sepsis,but the positive rote is low.Indicators in diagnosis of sepsis included acute phase reaction proteins,cytokines,cell surface antigen and bacterial DNA testing.This article compares and analyzes the indicators in diagnosis of neonatal sepsis.

Glucocorticoid is one of the widely used drugs in clinic,especially in nephrotic syndrome.This paper mainly summarizes its use in nephrotic syndrome in clinic,focusing on its classification,different dosage,mechanisms,basic principles of application,the inhibition of adrenal axis and precautions.