Maxillary first molar with twin-root canal in palatal side is rare. This article described two cases of maxillary first molar with twin-root canal. A properly designed and prepared access of the cavity is helpful for the diagnosis and negotiation of the root canal morphology.
Dental Pulp Cavity ; Humans ; Maxilla ; Molar ; Root Canal Therapy

Objective To investigate possible nerological origination of rabbit P3a potential. Method we simultaneously recorded the ERPs at dura and various depths beneath the skull in a point of rabbit skull, similarly did some in different points of rabbit skull. Result In the Brodmann 25 and 32 areas(AcgI), the polarity of P3a to those at dura was markedly reverse and the reverse rate of P3a(81.3%) was significantly higher than other locations, the P3a without polarity reversal showed steep voltage gradient. There was an increasing trend of the P3a reversal and moderate voltage gradient nearby the AcgI, but not other regions. Conclusion Rabbit P3a potential possibly originate from the AcgI acea.

Objective To evaluate and compare the advantages and disadvantages and the clinical application between transvaginal abdominal hysterectomy(TAH)and laparoscopically assisted vaginal hysterectomy(LAVH)on the benign lesions of non-prolapsed uterus.Methods 400 patients with benign lesions of non-prolapsed uterus were divided into two groups,TAH group(202 cases)and LAVH group(198 cases).The effects of clinical treatment of the two groups were compared.Results There were significant differentces between the two groups in operation time,the blood loss,passage of gas by anus,return to normal activities,and overall cost of treatment.However,after 1to 3 months follow-up,all patients were good in terms of outcome.Conclusion Different surgical patterns of hysterectomy had their distinctive advantages.The key point of a successful operation Was to avoid complications of the operation.

ObjectiveTo explore medical students on the level of awareness of bronchoscopy,bronchoscopy teaching methods and teaching effectiveness. MethodsMaking a analysis of the appraisal questionnaire on the theoretical knowledge bronchoscopy study, bronchoscopy operational training of the intemship system of the clinical course. ResultsAfter clinical teaching of bronchoscopy, medical students have improved significantly. their operation skills and motivation in learning the respiratory system anatomy, pathological features, imaging diagnosis and bronchoscopy technology. ConclusionThe clinical teaching of bronchoscopy can improve the quality of clinical teaching in respiratory medicine, which helps to improve the clinical skills of medical students, and lay the foundation for training medical students in bronchoscopy.

We have made a cheap microsystem of capillary electrophoresis with a new method, integrating the electrodes, injection channel, separation channel, buffer reservoirs and detection cell on a polymethylmethacrylate (PMMA) chip. Using an integrated micro carbon fiber disk electrode as the working electrode in three electrodes system, 1 × 10-4 mol/L dopamine (DA) could be detected with end-column amperometric detection. The reproducibility was good. Peak current was 6.73 nA、theoretical plate numher was 71300/m and height equivalent of one theoretical plate height was 14.0 μm for 1 × 10-4 mol/L DA. The limit of detection was 3.6× 10-8mol/L and the linear range was extended from 5 × 10-7 mol/L to 1 × 10-4 mol/L for DA. 1 × 10-4 mol/L catechol (CA) and 5× 10- 5 mol/L DA were also separated completely with R. = 10.1.

Methods involved in the treatment of malignant tumor are surgical therapy, radiotherapy, chemotherapy and immunotherapy. Although some effects have been showed in tumor therapy, the limits of single use of those methods can not be avoided. Therefore, combined therapy has been pursued in order to achieve better results. The effects of those combined methods such as chemotherapy combined with cytokine, chemotherapy combined with tumor vaccine, chemotherapy combined with adjuvants have been approved both experimentally and clinically. These results, in some extent, will provide us a new way to fight against tumor.

Objective:To analyze the infection results of The immunofluorescence detection of respiratory syncytial virus (RSV) in huazhou region from 2009 to 2010.Methods: The N gene sequence of RSV was taken as a reference to design specific primers and TaqMan fluorescent probe and to establish real-time The immunofluorescence method to detect sensitivity and specificity.21548 swab samples of children with acute respiratory infections in huazhou region from 2009 to 2010 were tested with real-time fluorescence. The immunofluorescence method.Results: The specificity and sensitivity of real-time fluorescence. The immunofluorescence method for detection of RSV infection were higher, without cross-reactivity to other respiratory viruses.10,477 cases of clinical specimens in 2009 were detected, with positive RSV infection rate of 9.73%, by real-time The immunofluorescence method detection. Positive detection rate of RSV infection had two peaks, which lied in February and December; the positive rate was 11% and 11% respectively. RSV infection rate was the highest for children under 6 years old.Conclusion: Real-time fluorescence features convenient detection, high specificity, and high sensitivity, which can be used for clinical detection of RSV infection. From 2009 to 2010, RSV infection remains a major pathogen of acute respiratory infections in children in huazhou region.

Objective To investigate the effect of curcumin on the proliferation,cell cycle distribution and gene expression of human breast cancer MCF-7 cells.Methods MCF-7 cells were treated with 10,20,40,80 ?mol/L curcumin for 24,48 or 72 h.MTT was used to assay the biologic activities of curcumin and its antiproliferative effect.FCM was used to detect cell cycle distribution and apoptosis.The expression levels of bcl-2,bax,PCNA mRNA were analyzed by RT-PCR.Results The curcumin IC_(50)value for MCF-7 cells was(18.5 ?mol/L.) The inhibitory effect of curcumin on cell growth was in a time-and dose-dependent manner.MCF-7 cells treated with 20 ?mol/L or(40 ?mol/L) curcumin for 24 h were arrested in G_0/G_1 phase and induced apoptosis.The expression levels of bcl-2,PCNA mRNA were decreased in MCF-7 cells by 10,20 and 40 ?mol/L curcumin treatment for 24 h,and bax mRNA was increased.Conclusion Curcumin is of inhibitory effect on MCF-7 cell growth,and it can arrest MCF-7 cells in G_0/G_1 phase,inhibit the proliferation and induce apoptosis.Curcumin down-regulates bcl-2,PCNA mRNA expression in MCF-7 cells,but upregulates bax mRNA expression.

Object To investigate the ultrafine grinding and nanometer powder's antiobesity effect of Amorphophallus rivieri Durieu on nutritional obese rats. Methods The content of triglyceride, cholesterol and high density lipoprotein (HDL) of blood was measured by UV spectraphotometry method. The blood glucose was determined by spectraphotometry method. The antiobesity effect was determined by weight loss and Lee's coefficient. Results Compared with powder and polysaccharide of A. rivieri, the ultrafine grinding and nanometer powder of A. rivieri could significantly decrease the rat body weight and the Lee's coefficient of nutritional obese rats (P

Objective To investigate the generative and regulatory effects of AchMR on P 3a and its mechanism in different subregions of rabbit cingulate gyrus. Methods Event related potentials(ERPs) P 3a potentials in Normal, AcgI, AcgⅡand Pcg regions were recorded after microinfusion of 0.5 mmol/L scopolamine at three different time points(instantly, at the 15th minute and at the 30th minute), 2 mmol/L acetylcholine and 1 mmol/L scopolamine. Results The P 3a amplitude decreased and/or P 3a latency increased with scopolamine in time and dose dependent pattern in AcgⅠ, AcgⅡ and Pcg, and P 3a might disappear only in AcgⅠ and the P 3a again be recorded after microinfusion of acetylcholine in AcgⅠ. Conclusion The action of AchMR in AcgⅠ area markedly affects the generation and/or regulation of P 3a and confirms the AcgⅠ is the source of rabbit P 3a potential. The action of neurons in AcgⅠ might facilitate the P 3a potential.