Lymph node mapping, a hotspot in oncology and surgery in recent years, has been successful-ly used in the discovery and resection of the metastatic lymph nodes in patients with melanoma and breast cancer. For the complex of lymphatic drainage and the defect of tracers, lymph nodes mapping is still under investigation. As a new type of nano-materials, the quantum dots are used widely, the tumor lymph node mapping.

Bacterial colonization is a sign of bacterial infection in the hospital, and the bacterial colonization rate indicates the risk of bacteria infection. Newborn infection happen easily in the hospital. In recent years, sereral researches indicate that vaginal delivery, breastfeeding and probiotics usage and so on can lower the probability of intestinal pathogenic bacteria colonization, which can lower the probability of neonatal enterogenous infection. Therefore, there are many factors that can effect on newborn intestinal bacteria colonization, and because experimental conditions are limitated, so we have many problems to solove in the future.

Lyophilized storage of red blood cells(RBC) may provide a useful storage alternative Both shelf life and transportation logistics can be improved over those for existing refrigerated or frozen storage. The present study was designed to evaluate the effects of lyophilization process on mean RBC recovery. Blood was drawn from healthy adult having no history of either hemoglobinophathy or abnormal RBC metabolism. Blood stored in plastic transfer bags were lyophilized for 16 hours, then it was rehydrated . In group 1, samples were stored at 4℃ for 6 hours, while in group 2 were stored at 4℃ for 5 hours, then 25℃ for 1 hour. The mean RBCs recovery in group 2 was higher than group 1(81 4?7 6% vs 62?10 2%,respectively). Samples were lyophilized using buffer containing different concentrations of glucose, and studies showed that there was no significant difference in mean RBCs recovery between samples in lyophilization buffer containing high and medium concentration of glucose, but low concentration of glucose in buffer resulted in lower mean RBCs recovery. The mean RBCs recovery was 30% in low concentration of glucose buffer and 45 1%, 47 2% in medium and high concentrations of glucose buffer, respectively. We also observed effects of different rehydration buffer with different osmotic pressure, and it showed that high osmotic pressure buffer resulted in higher mean RBCs recovery than iso-osmotic buffer. There was little change in the cell size and cell volume. The results presented in this report clearly demonstrate that human RBCs can be successfully lyophilized. Lyophilization buffer and rehydration buffer may influence the mean RBCs recovery.

Objective To investigate the effect of changes in plasma lipid levels on the accumulation of glomerular extracellular matrix in type 2 diabetic rats.Methods Rats were divided into three groups,namely,normal control,diabetes mellitus treated with Fenofibrate,which was gastrically administrated in the dose of 20mg?kg -1 ?d -1 for 22 weeks.A quantitative analysis of the components of glomerular extracellular matrix (ECM) were performed with immunoperoxide (ABC) method and the computer imagine analysis system.Results The result showed that diabetic rats had significantly higher plasma lipid levels and accumulation of ECM including collagen Ⅳ,laminine and fibronectin than the normal control ( P

Objective To study the immune function of gynecologic malignant tumor patients with chemotherapy and the influence of cord blood transfusion.Methods Thirty cases of gynecologic malignant tumor patients with chemotherapy and 30 of healthy women were divided into three groups,MTT colorimetry and flow cytometry was used to determine the change of immune function.Results ①The number of CD4 + T cell in patients was lower than that in healthy women and CD8 + was much higher.The ratio of CD4 +/CD8 + was inverted( t=2.56,P

Objective To evaluate the value of sterotactic mammorgraphy core needle biopsy in the diagnosis of nonpalable breast lesion. Methods 32 cases in this study were diagnosed by SMCNB,of whem,21 cases were operated on.Pathologic findigs of the tissues through SMCNB were compared with those of tissues by operation. Results Accuracy of diagnosis from SMCNB was 95%,sensitivity 80%,and specificity 100%.False negative result was found in 1 case.No false positive result occurred. Conclusions SMCNB is a simple and less invasive procedure with accurate location.It is valuable in the diagnosis of nonpalpable breast lesions.

The bark of Cinnamomum cassia Presl can be regenerated after peeling by painting with auxin (NAA ) and 6-benzyl amido Purine and dressed with plastic films. Mechanism regener ation and its economical potential need further rescarch

AIM To establish the method for quality control of Qiwei Ketenzi Pill (Semen Entadae, Semen Nigellae, Fructus Piperis, ect). METHODS Semen Nigellae was identified by TLC, and vitexicarpin was determined by HPLC. RESULTS TLC spots developed were fairly clear, and the bland test showed no interference. vitexicarpin in Qiwei Ketenzi Pill showed a good linear relation in the concentration range of 0.106 5 - 2.130 0 ?g and the average recovery was up to 101.4 % , RSD was 1.34 T《B》 % . CONCLUSION The method is effecive for the quality control of Qiwei Ketenzi Pills.

Objective To determine the origin of cord serum leptin and its relationship with neonatal anthropometry. Methods Sixty five women and their babies took part in this prospective cohort study. Blood was taken from the women just before delivery and from the umbilical cord of their babies at delivery. Serum leptin was measured by radio immunoassay. Neonatal anthropometric measurements were recorded within 48 hours after delivery. Linear regression analysis was used to explore the relationship between serum leptin concentrations and anthropometric measures and multiple regression analysis then applied to determine which variables remained independently associated with leptin. Results The leptin concentration ( ?s )in maternal serum was (19.93?7.21) ng/mL and in cord blood was (10.50?3.45) ng/mL. Cord leptin levels correlated with placental weight, neonatal birthweight, skinfold thickness and ponderal index but not with maternal leptin levels. The correlation with Placental weight and neonatal birthweight remained significant after multiple regression analysis. Conclusions Relatively big serum leptin concentration gradient between mother and umbilical vessels indicates that placenta might play an important role in leptin production. We hypothesize that leptin might play an important role during pregnancy and fetal development.

Objective: To investigate the effects of endothelin -1(ET-1) in the process of oral carcinogenesis. Methods: A sensitive SABC immuno-histochemical method and image pattern analysis technique were adopted in the examination of the localization and content of ET-1 in human oral mucosa from 10 cases of oral submucous fibrosis (OSF), 9 of oral leukoplakia (OLK),14 of oral squamous cell carcinoma(SCC)and 10 of normal control(NOR). Results: (1)Increaed expression of ET-1 was observed in the epithelial cells in OSF, OLK and SCC.The immune reative material was prominent within the cytoplasm and on the cell membrane of spinocells and basiocells.The proportion of ET-1 positive cells and cellular content of ET-1 in OSF,OLK and SCC were all significantly higher than that in NOR (P0.05). Conclusion: The cellular content of ET-1 probably increases in the process from premalignance to malignance.Its overexpression in OSF possibly indicates the potential malignance of epithelial cells.ET-1 may play a possible role in the pathogenesis of OSF,OLK and SCC.