Bacterial colonization is a sign of bacterial infection in the hospital, and the bacterial colonization rate indicates the risk of bacteria infection. Newborn infection happen easily in the hospital. In recent years, sereral researches indicate that vaginal delivery, breastfeeding and probiotics usage and so on can lower the probability of intestinal pathogenic bacteria colonization, which can lower the probability of neonatal enterogenous infection. Therefore, there are many factors that can effect on newborn intestinal bacteria colonization, and because experimental conditions are limitated, so we have many problems to solove in the future.

Lymph node mapping, a hotspot in oncology and surgery in recent years, has been successful-ly used in the discovery and resection of the metastatic lymph nodes in patients with melanoma and breast cancer. For the complex of lymphatic drainage and the defect of tracers, lymph nodes mapping is still under investigation. As a new type of nano-materials, the quantum dots are used widely, the tumor lymph node mapping.

Lyophilized storage of red blood cells(RBC) may provide a useful storage alternative Both shelf life and transportation logistics can be improved over those for existing refrigerated or frozen storage. The present study was designed to evaluate the effects of lyophilization process on mean RBC recovery. Blood was drawn from healthy adult having no history of either hemoglobinophathy or abnormal RBC metabolism. Blood stored in plastic transfer bags were lyophilized for 16 hours, then it was rehydrated . In group 1, samples were stored at 4℃ for 6 hours, while in group 2 were stored at 4℃ for 5 hours, then 25℃ for 1 hour. The mean RBCs recovery in group 2 was higher than group 1(81 4?7 6% vs 62?10 2%,respectively). Samples were lyophilized using buffer containing different concentrations of glucose, and studies showed that there was no significant difference in mean RBCs recovery between samples in lyophilization buffer containing high and medium concentration of glucose, but low concentration of glucose in buffer resulted in lower mean RBCs recovery. The mean RBCs recovery was 30% in low concentration of glucose buffer and 45 1%, 47 2% in medium and high concentrations of glucose buffer, respectively. We also observed effects of different rehydration buffer with different osmotic pressure, and it showed that high osmotic pressure buffer resulted in higher mean RBCs recovery than iso-osmotic buffer. There was little change in the cell size and cell volume. The results presented in this report clearly demonstrate that human RBCs can be successfully lyophilized. Lyophilization buffer and rehydration buffer may influence the mean RBCs recovery.

AIM To establish the method for quality control of Qiwei Ketenzi Pill (Semen Entadae, Semen Nigellae, Fructus Piperis, ect). METHODS Semen Nigellae was identified by TLC, and vitexicarpin was determined by HPLC. RESULTS TLC spots developed were fairly clear, and the bland test showed no interference. vitexicarpin in Qiwei Ketenzi Pill showed a good linear relation in the concentration range of 0.106 5 - 2.130 0 ?g and the average recovery was up to 101.4 % , RSD was 1.34 T《B》 % . CONCLUSION The method is effecive for the quality control of Qiwei Ketenzi Pills.

Objective: To investigate the effects of endothelin -1(ET-1) in the process of oral carcinogenesis. Methods: A sensitive SABC immuno-histochemical method and image pattern analysis technique were adopted in the examination of the localization and content of ET-1 in human oral mucosa from 10 cases of oral submucous fibrosis (OSF), 9 of oral leukoplakia (OLK),14 of oral squamous cell carcinoma(SCC)and 10 of normal control(NOR). Results: (1)Increaed expression of ET-1 was observed in the epithelial cells in OSF, OLK and SCC.The immune reative material was prominent within the cytoplasm and on the cell membrane of spinocells and basiocells.The proportion of ET-1 positive cells and cellular content of ET-1 in OSF,OLK and SCC were all significantly higher than that in NOR (P0.05). Conclusion: The cellular content of ET-1 probably increases in the process from premalignance to malignance.Its overexpression in OSF possibly indicates the potential malignance of epithelial cells.ET-1 may play a possible role in the pathogenesis of OSF,OLK and SCC.

Objective: To establish the determination of Shexianbaixin Pill. Methods: HPLC was applied to determine cinobufagin and resibufogenin. The determination was carried out using an ODS column with a solvent system: acetonitrile 0.5KH 2PO 4. The flow rate was 1.0mL?min -1 , detection wavelength was at 296nm and the column temperature was at 35℃. Results: The linear ranges of cinobufagin and resibufogenin were 0.148?g～0.746?g and 0.116?g～0.580?g, respectively. The average recoveries of cinobufagin and resibufogenin were 100.90%( RSD =1.004%, n =5), and 100.64%( RSD =1.006%, n =5), respectively. Conclusion: This method is simple, accurate with strong specificity and can be used to control the quality of Shexiangbaoxin Pill.

Objective To replace the imported vacuum pump with a Chinese-made instrument using a micro-ELISA negative pressure pump,to further extend the life of the machine.Methods Electric aspirator(YB.Dx23D) was used as source of negative pressure to connect with barrels of waste and the vacuum-phase of imported enzyme immunoassay instrument.The circuit was transformed,and the host matched the load,so the host and negative pressure pumps worked simultaneously.Results Compared with other aspirators,the modified electric aspirator YB.Dx23D has low-noise ratio,high negative pressure,vast flow,small volume,and mobile function.Conclusion The modified aspirator YB.Dx23D electric instrument is well used in washing ELISA plates and the effective use of waste water.Meanwhile,it can be applied to other necessary suction negative pressure(vacuum) equipment.

Objective To explore the relevant factors of hematoma enlargement in patients with hypertensive intracerebral hemorrhage(CH) at acute stage.Methods The clinical data of 208 patients with CH(29 patients with hematoma enlargement) were retrospectively analyzed.Results 27 cases(93.1%)of hematoma enlargement group onset at ≤ 24 h after CH.Compared with non-hematoma enlargement group,systolic blood pressure in the hematoma expansion group was significantly increased;and the rates of Glasgow coma scale(GCS) score decreased,basal ganglia hemorrhage,hepatic dysfunction,long-term alcohol drinking,taking Aspirin and diabetes in hematoma expansion group were significantly higher than those in the non-hematoma enlargement group(P

Objective To investigate the effect of changes in plasma lipid levels on the accumulation of glomerular extracellular matrix in type 2 diabetic rats.Methods Rats were divided into three groups,namely,normal control,diabetes mellitus treated with Fenofibrate,which was gastrically administrated in the dose of 20mg?kg -1 ?d -1 for 22 weeks.A quantitative analysis of the components of glomerular extracellular matrix (ECM) were performed with immunoperoxide (ABC) method and the computer imagine analysis system.Results The result showed that diabetic rats had significantly higher plasma lipid levels and accumulation of ECM including collagen Ⅳ,laminine and fibronectin than the normal control ( P

Objective To study the immune function of gynecologic malignant tumor patients with chemotherapy and the influence of cord blood transfusion.Methods Thirty cases of gynecologic malignant tumor patients with chemotherapy and 30 of healthy women were divided into three groups,MTT colorimetry and flow cytometry was used to determine the change of immune function.Results ①The number of CD4 + T cell in patients was lower than that in healthy women and CD8 + was much higher.The ratio of CD4 +/CD8 + was inverted( t=2.56,P