OBJECTIVE: To establish a bacterial endotoxin test method for fluorescein sodium injection.METHODS: Based on the bacterial endotoxin test method stated in Chinese Pharmacopoeia 2005 edition(second part),an interference test was conduced on different batches of fluorescein sodium samples using tachypleus amebocyte lysate from different companies.RESULTS: At a concentration of no more than 0.75 mg?mL-1,fluorescein sodium showed no interfere with the bacterial endotoxin test.CONCLUSION: It is feasible for bacterial endotoxin test of fluorescein sodium injection be conducted in a limit value of 0.15 EU?mL-1.

Objective To establish the quality standard for Kangyuan Granula. Methods Radix Astragali, Radix Paeoniae Alba, Fructus Lycii and Radix et Rhizoma Glycyrrhizae in this prescription were identified by TLC. The content of the Paeoniflorin was determined by HPLC. The column of AichromBond-AQ-C18 (4.6 mm?150 mm, 5 ?m) was used, the mobile phase was Water-Acetonitrile (85∶15), at the flow of 1.0 mL/min, the column temperature of 30 ℃, and peaks were detected at 230 nm. Results The characteristic of identification by TLC was distinct and highly specific. The linear range of Paeoniflorin was 0.0545～0.545 ?g, r =0.999 5, the average recovery was 97.90%, RSD=1.06% (n =6). Conclusion The method was easy to operate with accurate result and good reproducibility. It can be used effectively for the quality control of this preparation.

Ischemic stroke is one of the major diseases of causing adult disability and death.Age,sex,smoking,hypertension,and diabetes,etc.are the risk factors for ischemic stroke,but they can only partially explain the reasons of stroke onset.Twins,families,and single-gene genetic stroke studies have shown that ischemic stroke has obvious genetic predisposition.In recent years,genomics has made remarkable progress in ischemic stroke study and has shown that many new single nucleotide polymorphisms or genes are associated with ischemic stroke and its risk factors.However,the contribution of these genetic genes for ischemic stroke is still too small and the repetitive studies are needed for further confirmation.

Currently targeted therapy of breast cancer therapy has become the hot research field and a kind of brand-new biological treatment mode after three traditional pattern of the surgery, radiotherapy and chemotherapy for breast cancer. Molecular targeted therapy is aimed at the target that may cause cancer cells, such as protocarcinogenic genes and tumor-suppressor genes, cell signaling pathways, cytokines and receptors, antiangiogenesis etc. It can reverse malignant biology behavior to inhibit tumor cell growth from the molecular level and has the advantages of high specific effects and low side effects. This paper focuses on the drug of the molecular targeted therapy for breast cancer and the latest progress of targeted therapy.

To Describe the current situation of Niger children receiving acupuncture treatment.Acupuncture has 30 years of development history in Niger.Chinese medical teams had established acupuncture department in the Central Hospital in Maradi,Niamey National Hospital,and National Hospital of Zinder,and presided over acupuncture work.But due to a variety of reasons,at present,only the Central Hospital in Maradi has reserved acupuncture therapy,and was combined with physical therapy,rehabilitation therapy to form exercise therapy and functional training division.Pediatric patients are the major subjects who received acupuncture,accounting for 2/3 of outpatients.Nervous system and motor system diseases were their main diseases.

Developmental language disorder is a common childhood developmental disability,language development of children with a clear backward,but ruled out the hearing impairment,mental retardation,severemental disorder,autism,and other factors influence on language development.The most prominent clinical manifestation is that the level of language development is significantly lower than the cognitive level.They have different language development characteristics.There might be defects in semantics,syntax,etc.This paper mainly describes from the etiology,clinical manifestations,diagnosis,and other aspects of research starus on children with developmental language disorder.

MicroRNA (miRNA),an endogenous RNA,is wide spread in various organisms.Recent studies have found that miRNA plays an important role in cell proliferation,differentiation,apoptosis,immune and inflammatory response,etc.It's estimated that nearly 1/3 genes are regulated by miRNA.Because of its important role,the studies of miRNA related to kidney physiological and pathological mechanisms attract more attention gradually.it's very necessary to summarize the latest studies on kidney diseases.

With the development of endocrine drugs,neoadjuvant endocrine therapy becomes the hotspot of the research on breast cancer.A lot of studies have proved that neoadjuvant endocrine therapy can degrade the tumor staging,increase the local control rate and breast-conserving rotes.And the therapeutic effect of the third generation aromatase inhibitor is superior to that of tamoxifen.

The laboratorians are trying to make better communication and collaboration with clinical professionals in variety of ways, and to make better service for the clinicians.

Laparoscopic radical resection of rectal neoplasms is still under clinical research currently due to the following 2 reasons. Firstly, compared with open surgery, the longterm efficacy of laparoscopic surgery remains unclear; secondly, the pelvic autonomic nerves are difficult to be exposed and easy to be damaged during the surgery under laparoscope. Till present time, our department has completed 800 cases of laparoscopic radical resection of colorectal neoplasms, in which rectal neoplasms accounted for 70%. For most cases, the pelvic autonomic nerves have been exposed and protected properly. This paper summarized the skills to protect the pelvic autonomic nerves in the laparoscopic total mesorectal excision used for the treatment of rectal neoplasms.