Ischemic stroke is one of the major diseases of causing adult disability and death.Age,sex,smoking,hypertension,and diabetes,etc.are the risk factors for ischemic stroke,but they can only partially explain the reasons of stroke onset.Twins,families,and single-gene genetic stroke studies have shown that ischemic stroke has obvious genetic predisposition.In recent years,genomics has made remarkable progress in ischemic stroke study and has shown that many new single nucleotide polymorphisms or genes are associated with ischemic stroke and its risk factors.However,the contribution of these genetic genes for ischemic stroke is still too small and the repetitive studies are needed for further confirmation.

OBJECTIVE: To establish a bacterial endotoxin test method for fluorescein sodium injection.METHODS: Based on the bacterial endotoxin test method stated in Chinese Pharmacopoeia 2005 edition(second part),an interference test was conduced on different batches of fluorescein sodium samples using tachypleus amebocyte lysate from different companies.RESULTS: At a concentration of no more than 0.75 mg?mL-1,fluorescein sodium showed no interfere with the bacterial endotoxin test.CONCLUSION: It is feasible for bacterial endotoxin test of fluorescein sodium injection be conducted in a limit value of 0.15 EU?mL-1.

Currently targeted therapy of breast cancer therapy has become the hot research field and a kind of brand-new biological treatment mode after three traditional pattern of the surgery, radiotherapy and chemotherapy for breast cancer. Molecular targeted therapy is aimed at the target that may cause cancer cells, such as protocarcinogenic genes and tumor-suppressor genes, cell signaling pathways, cytokines and receptors, antiangiogenesis etc. It can reverse malignant biology behavior to inhibit tumor cell growth from the molecular level and has the advantages of high specific effects and low side effects. This paper focuses on the drug of the molecular targeted therapy for breast cancer and the latest progress of targeted therapy.

Objective To establish the quality standard for Kangyuan Granula. Methods Radix Astragali, Radix Paeoniae Alba, Fructus Lycii and Radix et Rhizoma Glycyrrhizae in this prescription were identified by TLC. The content of the Paeoniflorin was determined by HPLC. The column of AichromBond-AQ-C18 (4.6 mm?150 mm, 5 ?m) was used, the mobile phase was Water-Acetonitrile (85∶15), at the flow of 1.0 mL/min, the column temperature of 30 ℃, and peaks were detected at 230 nm. Results The characteristic of identification by TLC was distinct and highly specific. The linear range of Paeoniflorin was 0.0545～0.545 ?g, r =0.999 5, the average recovery was 97.90%, RSD=1.06% (n =6). Conclusion The method was easy to operate with accurate result and good reproducibility. It can be used effectively for the quality control of this preparation.

Antioxidants ; metabolism ; therapeutic use ; Bronchopulmonary Dysplasia ; drug therapy ; etiology ; metabolism ; Humans ; Hyperoxia ; complications ; Infant, Newborn ; Lung ; metabolism ; Reactive Oxygen Species ; metabolism

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Guillain-Barre Syndrome ; therapy ; Humans ; Male

Objective To investigate the clinical efficacy and safety of phaocemulsification and intraocular lens combined with goniosynechialysis(Phaco + IOL + GSL) for treating the patients with primary angle closure glaucoma(PACG) with cataract.Methods 136 cases (141 eyes) PACG and cataract patients underwent the surgical treatment,their clinical data were retrospectively analyzed.67 cases(69 eyes) underwent Phaco + IOL + GSL surgical treatment(treatment group) and 69 cases (72 eyes) underwent Phaco + IOL + TBL surgical treatment (control group).The surgical treatment effects were compared and analyzed between two groups.Results At postoperative 1-month,3-months,6-months and 12-months,the intraocular pressure of treatment group was (13.32 ± 1.63) mm Hg,(14.43 ± 1.84)nn Hg,(14.65 ± 1.80) mm Hg and (14.73 ± 1.88)mm Hg respectively,the visual acuity was (0.423 ± 0.091),(0.472 ± 0.088),(0.615 ± 0.109) and (0.682 ± 0.127) respectively,the anterior chamber depth was (2.75 ± 0.59) mm,(2.97 ± 0.56) mm,(3.25 ± 0.58) mm and (3.41 ± 0.64) mm respectively.The intraocular pressure of control group was (17.35 ± 2.13)mm Hg,(16.87 ± 2.15) mm Hg,(16.31 ± 2.00) mm Hg and (15.68 ±2.02)ram Hg respectively,the visual acuity was (0.321 ±0.069),(0.362 ±0.062),(0.354 ±0.069)and (0.381 ±0.088) respectively,the anterior chamber depth was (2.21 ±0.48)mm,(2.35 ±0.40)mm,(2.41 ±0.47)mm and (2.43 ± 0.56)mm respectively.Compared with the control group,the intraocular pressure significantly reduced,visual acuity significantly increased and anterior chamber depth significantly increased of treatment group in posto perative 1-month,3-months,6-months and 12-months(all P ＜0.05).There were no serious complications in the two groups.Concluslon The triple therapy of Phaco + IOL + GSL has better clinical efficacy and security,and the therapy is worthy of clinical application.

Objective To evaluate the clinical efficacy of procedure for prolapse and hemorrhoids (PPH)plus Milligan-Morgan methods for circumferential mixed hemorrhoid.Methods A total of 74 cases with circumferential mixed hemorrhoid were treated by PPH plus Milligan-Morgan methods.Results The anal form of all cases were changed correctly.There was no complication such as anal edema,incontinence,anal stenosis,postoperative massive bleeding and infection.Conclusion PPH plus Milligan-Morgan methods is a safe and effective way for circumferential mixed hemorrhoid.This way can decrease postoperative complications and improve the curative effect.

Objective To explore the clinical value of early arterial phase (EAP) enhanced imaging with MSCT in detecting liver focal lesions.Methods 100 patients with liver focal lesions were examined with MSCT scan.The images were analyzed.Results There were 85.0％ of the 100 cases that demonstrated EAP enhancement,which was hemangioma of liver(100.0％),hepatapostema (100.0％),hepatocellular carcinoma (95.0％),liver metastasis (84.0％),and cholangiocellular carcinoma(25.0％) in descending order.The abnormal vascular lesions and heterogeneous enhancement had high sensitivity and specificity for hepatocellular carcinoma,and the edge nodular enhancement for hemangioma,and the ring enhancement for metastases.Conclusion The EAP enhanced imaging with MSCT in detecting liver focal lesions has great applicative value.

Medical institutes in the United States,the United Kingdom and Canada have systematically explored and published standard of physician competence,such as ACGME six-critical-components,GMC good medical practice and CanMEDS,which has led to changes in the content,item format and clinical skill test of foreign medical incensing exam.Foreign research inspires Chinese counterparts to study and develop the Chinese standard of physician competence so as to continuously enhance the medical licensing exam.