Brain ; growth & development ; Child Development ; Humans ; Infant Behavior ; Infant, Newborn ; physiology ; Motor Activity ; Neonatal Screening ; Nerve Net ; Neurologic Examination

Aged ; Asthma ; complications ; drug therapy ; physiopathology ; Coal Mining ; Humans ; Immunoglobulins, Intravenous ; administration & dosage ; therapeutic use ; Infusions, Intravenous ; Male ; Middle Aged ; Pneumoconiosis ; complications ; drug therapy ; physiopathology ; Respiratory Function Tests ; Treatment Outcome

The aim of this study was to investigate the effect of total parenteral nutrition(TPN), infection, or growth hormone on changes in proliferation of intestinal mucosa epithelial cells and expression of mucosa c jun . Cecal ligation and puncture was used to replicate intra abdominal infection model, and transjugular intracaval catheterization was chosen to give TPN. Experimental rats were divided into four groups: normal control, TPN control, TPN plus sepsis group, and TPN plus growth hormone group. Changes in mucosa thickness, villus height, crypt depth , mucosa c jun mRNA expression and epithelial cell proliferation index were observed. The results showed that all of mucosa thickness, villus height, crypt depth of intestinal mucosa decreased, proliferation of epithelial cells were markedly inhibited in TPN group. Sepsis states aggravated the above changes in intestinal mucosa. Corresponding to mucosa atrophy, expression of c jun reduced obviously. Growth hormone could promote growth of intestinal mucosal cell, improve proliferation of epithelial cells and expression of mucosa c jun. It suggested that changes in c jun expression appeared to be associated with different proliferation states of intestinal mucosa epithelial cells. c jun mRNA could be used as an important marker to reflect mucosa epithelial cell proliferation .

The aim of this study was to observe the changes in tissue suppressors of cytokine signaling (SOCSs) mRNA expression, and to investigate their potential role in the pathogenesis of sepsis induced by cecal ligation and puncture (CLP). Fifty four Wistar rats were randomly divided into three groups: normal control group ( n =6), CLP induced sepsis group ( n =36) and recombinant bactericidal/ permeability increasing protein (rBPI 21 ) treatment group ( n =12). Tissue samples from the liver, lung and kidney were collected to determine SOCS1 and SOCS3 mRNA expressions. Meanwhile, tissue endotoxin and TNF ? levels were also determined. The results showed that, after CLP, endotoxin and TNF ? levels in the liver, lung and kidney significantly increased, peaking at 2～12h ( P

The aim of this study was to investigate changes in high mobility group 1 (HMG 1) protein in postburn Staphylococcus aureus infection and its potential signaling mechanism. A total of 61 male Wistar rats were randomly divided into four groups as follows: normal control group ( n =6), scald control group ( n =9), postburn sepsis group ( n =36) and rapamycin (RPM) treatment group ( n =10). Tissue samples from the liver and kidney were collected to determine tumor necrosis factor ? (TNF ?) and HMG 1 mRNA expression. The results showed that, after Staphylococcus aureus challenge, TNF ? mRNA expression was up regulated rapidly in both the liver and the kidney, peaking at 2 hours ( P

To study the formation and change of platelet leucocyte aggregation (PLA) in red blood cell concentrations(CRCs) stored at 4℃ and the preventive effectiveness of filtration, 8 units of CRCs were each divided into two equal parts in two integrative bags and two paired groups were formed: control group and filtration group. The following items of control group and filtration group were detected weekly. Platelet and leukocyte counts, concentration and percentage of platelet lympocyte (P lym), platelet monocyte (P mon), platelet neutrophil (P neu) aggregatoins, and concentration of total PLA. The results showed that rate and concentration of PLA in the control group were significanly higher than those in the circulating blood. The rates of P lym and P mon in CRCs stored at 4 ℃ were two to five times of those before storage, and the rate of P neu in CRCs stored at 4 ℃ was ten to twenty times of that before storage. PLA in the CRCs separated from plasma rose to a high level at the first day of preparation. The concentration of PLA in CRCs reached the highest level at one week after stored at 4 ℃ and then decreased, but a high concentration was retained all the while. P neu is the major part (about 80%～90%) of whole PLA in CRCs. PLA was not detectable by flow cytometry during the whole storage course. The results suggested that high concentrated PLA was detected in CRCs stored at 4 ℃, which could be prevented by leucocyte depleting filtration.

Objective To improre typieal proeedure of UPPP,increase clinical effects and deerease complioations caused by the operation.Methods Twenty eight patients with obstructive sleep apnea syndrome who had been confirmed by PSG in our hospital from 2003 to 2005 were involved in this study.1)improving stripping method:the fat and tonsil were removed by electric knife.2)improving molding method:the muscle of uvula was maitained,but the fat was removed;mucous and the tissue under it were double-layer sutured.3) the data before and 6 months after UPPP were analyzed by questionnaire and polysomnography(PSG)detection.Result No fissuration occurred.The distance between soft palate and posterior wall was 3?0.6 before operation and it was 6?0.8 6 months after operation.The effect is 100% based on the questionnaire investigation and PSG detection.Conclusions 1)Hemostasis of electric knife was reliable;2)Double-layer suture of mucous and the tissue under it can avoid hemorrhage and insure molding of pharyngeal cavity;3)The results of the uvula preservation can improve the effect of the UPPP by increasing the nasopharyngeal and oropharyngeal cavity,and avoiding velopalatal insufficiency.

Objective To investigate the microcosmic pathologic mechanism of ac upoint application for asthma. Methods Sixty guinea pigs were randomly alloca ted to three groups: Group A (model control), Group B (guinea pig model treated with acupoint application) and Group C (normal control). Group B was treated wit h the application of herbal medicines on the acupoints located at neck and back. Results From the 6th day of treatment, incubation period of nodding breath was prolonged in Group B as compared with Group A (P

AIM: To study the effects of cyproheptadine (Cyp) and anisodamine (Ani)on the changes of intracellular free Ca 2+ concentration ([Ca 2+ ] i) induced by tumor necrosis factor (TNF ?) in single endothelial cells, and to explore the mechanisms of TNF ?-mediated shock and antishock actions of Cyp and Ani. METHODS: Human umbilical vein endothelial cell strains(ECV304) were seed in 35 mm tissue culture dish with 2 mL DMEM culture medium. The cultured cells were loaded by Fluo-3/AM. The spatial distribution and the dynamic changes of [Ca 2+ ] i in single endothelial cell was determined by laser scanning confocal microscopy(LSCM). RESULTS: [Ca 2+ ] i in single endothelial cell after stimulation of TNF ? rapidly increased in a dose-dependent manner and approached the peak value within 60 seconds, afterwards, decreased and kept above the basal level. The confocal scanning image showed that [Ca 2+ ] i elevation was more obvious in nuclear than in cytoplasma, and decreased slowly. Cyp (3?10 -5 , 6?10 -5 mol/L) and Ani (2?10 -5 , 4?10 -5 mol?L -1 ) markedly inhibited TNF ? (1.2?10 -9 mol?L -1 )-induced [Ca 2+ ] i elevation. CONCLUSIONS: TNF ? markedly induces elevation of [Ca 2+ ] i in single endothelial cell, it may be an important mechanism of TNF ?-induced shock and tissue injury. Cyp and Ani obviously suppress TNF ?-induced [Ca 2+ ] i elevation, which probably is one of the mechanisms of their antishock effects.

Objective:To explore the way to separate and culture eutopic and ectopic endometrial glandular cells and their stromal cells,providing an in vitro cell model of endometriosis for study of its mechanism. Methods:Digestion,filtration and sedimentation were used to isolate and culture the eutopic and ectopic endometrial glandular cells and their stromal cells. The estrogen level was imatated to study the way of promoting cell growth. Morphological characters of eutopic and ectopic endometrial cells were examined using optical microscope. Results:The success rate of separation and culture of normal control endometrial glandular cells and its stromal cells was 91.7%(11/12);of eutopic endometrial glandular cells and its stromal cells of endometriosis was 93.8%(15/16);of etopic endometrial glandular cells and its stromal cells of endometriosis was 75.0%(12/16) . Conclusion:The cultured eutopic and ectopic endometrial cells is more like human body feature than the endometriosis model of animals. So the isolation and culture of eutopic and ectopic endometrial glandular cells and their stromal cells may serve as an in vitro experimental model.