ObjectiveTo observe the changes in plasma interleukin-33 (IL-33) in patients with sepsis and its regularity, the association between IL-33 and the infection, and the significance of IL-33 in predicting the prognosis of sepsis.Methods A prospective single-center single-blind clinical study was conducted. Forty patients with sepsis in intensive care unit (ICU) of Shengjing Hospital of China Medical University from May 2012 to January 2013 were enrolled. The patients were divided into general sepsis, severe sepsis and septic shock groups according to the severity of systemic infection and presence of organ dysfunction. The sepsis patients were again divided into 28-day death group and survival group. Ten healthy volunteers and 11 patients with systemic inflammatory response syndrome (SIRS) were enrolled as healthy control and SIRS groups. The levels of procalcitonin (PCT), IL-33, IL-6, IL-1β, tumor necrosis factor-α (TNF-α), and IL-33 receptor sST2 were determined with enzyme linked immunosorbent assay (ELISA) within 3 hours, and 24 hours and 5 days after enrollment to ICU. The acute physiology and chronic health evaluationⅡ (APACHEⅡ) score was calculated. The clinical outcome, length of stay in ICU, and duration of mechanical ventilation were recorded. The relationship between IL-33 and each parameter was analyzed by Spearman analysis. Receiver operating characteristic (ROC) curve was drawn to evaluate IL-33 in predicting the outcome of sepsis.Results Plasma IL-33 in sepsis patients within 3 hours after admission was significantly increased compared with that of the healthy controls and SIRS group (ng/L: 15.43±7.22 vs. 0.67±0.24, 1.25±1.09, bothP< 0.01). Compared with SIRS group, PCT in sepsis group was significantly increased [μg/L: 52.23 (19.69, 73.37) vs. 1.22 (0.69, 3.73),Z = -2.447,P< 0.001]. With exacerbation of illness, APACHEⅡ score, the values of PCT and IL-33 were gradually increased in general sepsis, severe sepsis and septic shock groups, while the length of stay in ICU and the duration of mechanical ventilation were gradually prolonged (P< 0.05 orP< 0.01). The concentration of IL-33 (ng/L) of sepsis patients admitted to ICU within 3 hours, and 24 hours and 5 days of the illness was 15.43±7.22, 11.82±6.16, 5.55±2.25, respectively (F = 4.823,P = 0.004). There was a positive correction between IL-33 within 3 hours after ICU admission and APACHEⅡ score (r = 0.351,P = 0.031), PCT (r = 0.412,P = 0.005), IL-6 (r = 0.535,P = 0.030), IL-1β (r = 0.674,P = 0.030), TNF-α (r = 0.250,P= 0.030), sST2 (r = 0.620,P< 0.001), and length of stay in ICU (r = 0.296,P = 0.013), duration of mechanical ventilation (r = 0.385,P = 0.011). Decreased plasma IL-33 level could be found in the survivors (n = 37,F = 7.798,P< 0.01), and its level in non-survivors (n = 3) was increased (F = 37.283,P> 0.05). The area under the ROC curve (AUC) of IL-33 and PCT in ROC curve were 0.821, 0.829. When the cut-off value of IL-33 was 13.79 ng/L, the sensitivity was 74.2%, the specificity was 79.6%; when the cut-off value of PCT was 4.70μg/L, the sensitivity was 87.5%, and the specificity was 81.4%.Conclusions The concentration of IL-33 3 hours after ICU admission was obviously increased in sepsis patients, and it was positively correlated with PCT, therefore it is valuable in the diagnosis of the infection. In addition plasma IL-33 is related to the severity of sepsis. Its trend of change is valuable in predicting the outcome and in distinguishing sepsis from SIRS.

OBJECTIVE:To compare antidiuretic activity of Ootheca Mantidis before and after processing,and to explore the best medicinal part and mechanism of Ootheca Mantidis. METHODS:96 rats were randomly divided into blank group,model group,positive group,Ootheca Mantidis group,Ootheca Mantidis stir-fried with salt group,steamed Ootheca Mantidis group, crude product eggs and egg shell groups,processed product eggs and egg shell groups,with 8 rats in each group,12 groups in to-tal. Except blank group,other groups were given adenine 250 mg/kg,ig,for 4 weeks to induce kidney-yang and diuresis model. From third week,Ootheca Mantidis crude drug group and processed Ootheca Mantidis group were all given relevant medicine 0.11 g(crude drug)/ml i.g,and crude product eggs and egg shell groups and processed product eggs and egg shell groups were given rel-evant medicine,ig,once a day,by mass ratio of eggs to egg shell(cude drug 1∶2.4,salt stir-fried product 1∶1.7,steamed prod-uct 1∶2.1)for consecutive 4 weeks. The urinary volume,body weight,renal index and the serum contents of ADH and ALD were all determined. RESULTS:Compared with blank group,body weight and serum content of ADH and ALD decreased in model group,while renal index and urinary volume increased(P<0.05). After 4 weeks of treatment,compared with model group,body weight and serum content of ADH increased in Ootheca Mantidis groups,while urinary volume and renal index decreased (P<0.05);serum content of ALD increased in treatment groups;there was statistical significance in the serum content of ALD in those groups except Ootheca Mantidis group,Ootheca Mantidis stir-fried with salt group and steamed Ootheca Mantidis group (P<0.05);except for ALD,those index were in descending order of steamed Ootheca Mantidis group>Ootheca Mantidis stir-fried with salt group>Ootheca Mantidis group,and steamed Ootheca Mantidis shell group had best exchange. CONCLUSIONS:The an-tidiuretic activity of Ootheca Mantidis has been enhanced after processing. The egg shell of steamed Ootheca Mantidis is main me-dicinal part. To increase the serum content of ADH might be one of the main mechanism of arresting polyuria.

Actinomyces ; growth & development ; Coinfection ; Humans ; Maxillary Sinus ; Maxillary Sinusitis ; diagnosis ; microbiology

Female ; HIV Infections ; prevention & control ; transmission ; Humans ; Infant, Newborn ; Infectious Disease Transmission, Vertical

Objective To evaluate the clinical effect of intracavitary microwave hyperthermia combined with radiotherapy for advanced cervical cancer. Methods 66 patients were randomized into two groups:33 pa-tients treated by radiotherapy plus hyperthermia ( H + R group) ,33 patients treated by radiotherapy alone ( Rgroup). Radiotherapies of both groups were alike:a midplane dose of 30 Gy to the whole pelvis and 20 Gy to the parametrical areas were given by using 8 MV-X rays, combined with 42 Gy with 192Ir high dose rate(HDR)intracavitary brachytherapy. Intraluminal 915 MHz microwave hypertherrrfia was given 30 rain to 1 h after radio-therapy. The temperature was increased and maintained at 43-45℃ for 45 minutes, once or twice a week for 7-10 times. Results The rates of local control and infection in H + R and R groups were 87.88% and 63.63%,15.15% and 36.36% respectively. The differences of the two groups were statistically significant( P <0.05).In H + R group, the level of CD4/CD8 and NK cells after being treated were higher than those before being treated and that of R group( P <0.05). The rates of 2-year disease-free survival and 3-year survival for H +R and R groups were 93.94% and 72.72%, 84.85% and 60.61% respectively. The differences of the two groups were statistically significant( P<0.05 ). The rates of bone marrow inhibition and radiatory rectum inju-ry were lower in H + R group than those in R group. Conclusion Hyperthermia plus radiotherapy can improve local control rate and survival rate in advanced cervical cancer.

The factor and question about equipment purchase approval and management were discussed in this paper.Solutions of how to improve the efficiency and transparence on the equipment purchase and management were put forward.According to this,A Manage System had been designed which made the equipment application,demonstration,verification,bidding,checking and accepting become fluency and dynamic.All the data can be checked from the computer.The working course state is clear at a glance.

Objective To evaluate the clinical curative effect of the non-muscle invasive bladder cancer by selective green laser.Methods 80 cases of non-muscle invasive bladder cancer clinical data were retrospective analysis who received selective green laser treatment.Results All operations were successful,surgery durations were 15-120 min.Patients had basically no intraoperative bleeding,no obturator nerve reflex,without severe complication such as electricity cut syndrome.Postoperative applied of pirarubicin bladder perfusion chemotherapy.All patients were followed-up for 3 months-1 year.No recurred tumor was observed.Conclusion Transurethral selective green laser treatment of non-muscle invasive bladder cancer should be efficient and curative treatment,no serious complications are observed.

In order to provide some more scientific and practical advice and tactics for acupuncture in prevention of migraine in clinic, a computer-based information search was conducted in MEDLINE database, and the current reports of clinical trials were summarized and a comparison of Western and Chinese research designs was made. The significant role of acupuncture for prevention of migraine has not been applied in wide range in China until the present day. However, it has been paid great attention in Western countries. This situation might be related to the differences of efficacy assessment of acupuncture between China and Western countries; meanwhile, in the past traditional acupuncturists put much stress on the therapeutic approaches for acute attacks of headache but less on prevention. It is necessary for Chinese doctors to learn from research method in the West and emphasize more on acupuncture for preventing migraine and conduct high-level clinical trials as well. The author recommends that Chinese doctors should select acupuncture points mainly according to meridian syndrome differentiation and apply long term intermittent interventions.

The concepts of monomer medicine and the Chinese traditional medicine were introduced in this article. The different effects of a monomer drug between different types of syndromes of traditional Chinese medicine (TCM) were explained based on the author's clinical experience and related literature. The aim, significance and methods about the application of western medical drugs according to the theory of TCM were pointed out. And the feasibility of the application was analyzed.

Objective To evaluate the efficacy of hyperbaric oxygen in treatment of sudden deafness and to analyze related factors of deafness,in order to provide the basis for clinical treatment.Methods 72 cases with sudden deafness were admitted.41 cases (total of 43 ears) were given conventional treatment.31 cases (total of 32 ears) were given hyperbaric oxygen plus conventional treatment.The influencing factors of deafness in 32 cases were also analyzed.Results The total effective rate of hyperbaric oxygen therapy group was 81.25％,that of the conventional drug treatment group was 65.12％,the difference was statistically significant (P ＜ 0.05).In hyperbaric oxygen therapy group,the visiting time less than 24h,the effective rate was 100.00％,which was higher than visiting time 1 to 14 days (90.00％) and more than 15 days (37.50％) (P ＜ 0.05).The effective rate of visiting time between 10-20 days and more than 20 days was significant different (P ＜ 0.05).The effective rate from high to low was 15-34 years old,35-54 years old and ≥55 years old.The effective rate of 15-34 and 35-54 years old group was significantly higher than ≥55 group (P ＜ 0.05).The effective rate of mild deafness was 100.00 ％,which was significantly higher than moderate deafness 84.62％ and severe hearing loss 20.00％ (all P ＜ 0.05).Conclusion The effect of hyperbaric oxygen plus drug in treatment of sudden deafness was significant.The sudden deafness shoud be treated early in clinical by hyperbaric oxygen.