BACKGROUND:Occurrence and development of diabetic complications is closely related to the severity of oxidative stress imbalance in the body. OBJECTIVE:To investigate the effect of embryonic stem cel s on oxidative stress response of rats with diabetic nephropathy. METHODS:Primarily cultured rat embryonic stem cel s were observed for cel morphology and surface antigen detection. Sprague-Dawley rats were divided into experimental group (two injections of embryonic stem cel s via the tail vein), model group (injection of the same volume of PBS), and normal control group (with no modeling, intraperitoneal injection of sodium citrate-citrate buffer). In the former two groups, the rats were intraperitoneal y injected sodium citrate-citrate buffer diluted streptozotocin to establish diabetic nephropathy models before treatment. At 5 weeks after the last injection, blood glucose level, renal function indicators (urine protein/urine creatinine, blood urea nitrogen and serum creatinine) were tested in each group;contents of malondialdehyde and protein carbonyl were detected in the kidney;the expression level of superoxide dismutase was detected by western blot assay. RESULTS AND CONCLUSION:The embryonic stem cel s were oval or round, with clear boundary and good refraction, and highly expressed Oct-4 and SSEA-1. Compared with the control group, renal biochemical indicators, malondialdehyde and protein carbonyl contents were significantly increased, while the expression level of superoxide dismutase was decreased dramatical y in the model group and experimental group (P<0.05);compared with the model group, the renal biochemical indicators, malondialdehyde and protein carbonyl contents were dropped significantly in the experimental group, but the expression of superoxide dismutase was significantly rebounded (P<0.05). Taken together, embryonic stem cel s can reverse the occurrence and development of diabetic nephropathy by inhibiting oxidative stress in progress.

ObjectiveTo study the optimized security strategy for informing cancer patients with the disease,and let patients be fully informed and even more actively cooperate with doctors while they are under treatment.MethodsGrouped with adversity quotient (AQ) score,these cancer patients were in comparison with those who had been informed by other ways without support strategy,and different groups of patients were estimated in different strategies in accordance with self-rating anxiety scale (SAS) and self-rating depression scale (SDS).Results With certain strategies,high and low AQ score groups' SAS and SDS scores stably restored in 1 day to 1 week,and the differences had statistical significance(P ＜ 0.01 ).The patients were well-informed without any difficulty and actively cooperated with treatment.No related factors led to self-mutilation,suicide and other serious incidents.While the SAS and SDS scores of the control group who were not informed by the strategy were not satisfactorily recovered,and the patients could not actively cooperate with the doctors.Especially being notified after 1 d to 1 week,the SAS and SDS scores had no significant improvement,and the differences had no statistical significance compared with themselves ( P ＞ 0.05 ).It is difficult to avoid the occurrence of serious incidents.Conclusion Based on AQ score,it is safe and effective to inform cancer patients of their disease condition by individual strategies.It not only respects patients rights,but also gains the active cooperation of the patients,and is helpful for treatment with minimizing adverse effects.

Cardiovascular Diseases ; etiology ; Chronic Disease ; Humans ; Hypertrophy, Left Ventricular ; etiology ; Kidney Diseases ; complications ; Risk Factors

Objective To study the significance of serum human epididymis protein 4 ( HE4 ) and human cartilage glycoprotein-39(YKL-40) in the diagnosis of ovarian cancer .Methods 100 patients with ovarian cancer , 90 patients with benign tumor of ovary and 50 healthy people were enrolled .Serum HE4 and YKL-40 were detected . Results The positive rates of serum HE 4 in ovarian cancer group , ovarian benign tumor group and healthy group were 86.0%,5.5%,2.0%.The positive rate of serum HE4 in ovarian carcinoma was significantly higher than that in benign ovarian tumor group and the healthy group (χ2 =31.06,30.82,all P<0.01).The positive rates of serum YKL-40 in ovarian cancer group ,ovarian benign tumor group and healthy group were 82.0%,3.3%,2.0%.The positive rate of serum YKL-40 in ovarian carcinoma was significantly higher than that in benign ovarian tumor group and the healthy group(χ2 =30.92,32.06,all P<0.01).Serum HE4 in the diagnosis of ovarian cancer ,the sensitivity was 86.0%,specificity of 95.7%,accuracy of 91.7%.Serum YKL-40 in the diagnosis of ovarian cancer ,the sensitivity was 82.0%,specificity of 97.1%,accuracy of 90.8%.For the joint detection,the sensitivity for diagnosis of ovarian cancer was 92.0%,and the specificity was 93.6%,accuracy was 95.0%,higher than those of the single detection . Conclusion Combined with serum HE 4 and YKL-40 detection can significantly improve the diagnostic accuracy of ovarian cancer patients .

Objective To understand the diabetes drug application of 2012-2014 in Wujin People's Hospi-tal of Changzhou,and to provide reference for clinical medicine.Methods The information system in Wujin People's Hospital of Changzhou was used,anti diabetic drug use data were retrieved from 2012 to 2014 in our hospital.The sales volume of drugs,defined daily dose( DDD) ,drug DDDs,defined daily cost of DDDc and sorting ratio were ana-lyzed as index.Results Consumptions of antidiabetic drugs as insulin and its analogs in our hospital were between 400-500 million yuan,occupied about 70%of the proportion,and played a leading role.Alpha glucosidase inhibitors and metformin were the main component in the oral hypoglycemic drugs,accounted for 35% and 10% respectively;biguanides of DDDs value ordering three years in a row were in the first place,the DDDs of alpha glucosidase inhibi-tors value ordering three consecutive annual were averagely in the third and fourth.Conclusion The clinical medica-tion of anti diabetic drugs in Wujin People's Hospital of Changzhou is reasonable,safe and economic.

The paper introduces concept, value, content, composition and frame theory of the overall informatization framework, an-alyzes the requirements of regional health informatization, summarizes the main modes of regional health informatization construction in China and elaborates the overall framework of regional health informatization and positioning of each composing factors, in order to provide references for regional health informatization construction in various regions.

Taking the library of Beijing Obstetrics and Gynecology Hospital as an example, the paper analyzes the problems existed in literature access of medical personnel, discusses corresponding countermeasures, including constructing one-stop retrieval platform, establishing diversified information communication channel, innovating training mode, improving the quality of librarians, etc.

Ubiquitous library and ubiquitous service are the future trend of library.Described in this paper are thus the extended traditional service and multidimensional personal and deepened information service such as sub-ject service, academic conference information service, special subject navigation service, new foreign book transla-tion service, special subject information service, in order to let the readers use the library at any time and at any place and enjoy the library everywhere .

BACKGROUND:Dislocation is a common short-term complication after total hip arthroplasty. Many factors can induce dislocation. Among controlable factors, the diameter of the femoral head may be one of factors affecting the incidence of dislocation. Generaly, large prosthesis meets the biomechanical feature of hip joint, and can increase the joint stability. OBJECTIVE:To observe the effects of different diameters of the femoral head on range of motion of the hip joint during primary total hip arthroplasty so as to provide evidence for selecting the prosthesis with an ideal diameter. METHODS: We colected data of 77 cases (87 hips), who underwent primary total hip arthroplasty with the femora head of 28 mm and 32 mm diameter in the Department of Orthopedics, Inner Mongolia Autonomous Region Wulanchabu Central Hospital from August 2009 to August 2012. Hip prosthesis was provided by ZIMMER Company. The hip motion interface was made from polyethylene on metal. The femoral head prosthesis was used as folows: 28 mm diameter in 51 hips, and 32 mm diameter in 36 hips. Posterior approach was utilized. Range of motion of hip internal rotation and external rotation to dislocation was measured at hip flexion 90° during the operation, and compared between different diameter groups. RESULTS AND CONCLUSION:The method of surgery was not changed during operation in al patients. No significant difference in the sum of degree at dislocation was detected between two different diameter groups (P > 0.05). No significant difference in the intraoperative range of motion during total hip arthroplasty was visible in patients with femoral neck fracture between the two different diameter groups (P > 0.05). No significant difference in the intraoperative range of motion during total hip arthroplasty was visible in patients with avascular necrosis of the femoral head between the two different diameter groups (P > 0.05). These data suggested that compared with the femoral head of 28 mm diameter, the femoral head of 32 mm diameter did not increase the range of motion during total hip arthroplasty. After operation, two kinds of femoral head did not affect the dislocation after total hip arthroplasty in 2-year folow-up.