Cardiovascular Diseases ; etiology ; Chronic Disease ; Humans ; Hypertrophy, Left Ventricular ; etiology ; Kidney Diseases ; complications ; Risk Factors

BACKGROUND:Occurrence and development of diabetic complications is closely related to the severity of oxidative stress imbalance in the body. OBJECTIVE:To investigate the effect of embryonic stem cel s on oxidative stress response of rats with diabetic nephropathy. METHODS:Primarily cultured rat embryonic stem cel s were observed for cel morphology and surface antigen detection. Sprague-Dawley rats were divided into experimental group (two injections of embryonic stem cel s via the tail vein), model group (injection of the same volume of PBS), and normal control group (with no modeling, intraperitoneal injection of sodium citrate-citrate buffer). In the former two groups, the rats were intraperitoneal y injected sodium citrate-citrate buffer diluted streptozotocin to establish diabetic nephropathy models before treatment. At 5 weeks after the last injection, blood glucose level, renal function indicators (urine protein/urine creatinine, blood urea nitrogen and serum creatinine) were tested in each group;contents of malondialdehyde and protein carbonyl were detected in the kidney;the expression level of superoxide dismutase was detected by western blot assay. RESULTS AND CONCLUSION:The embryonic stem cel s were oval or round, with clear boundary and good refraction, and highly expressed Oct-4 and SSEA-1. Compared with the control group, renal biochemical indicators, malondialdehyde and protein carbonyl contents were significantly increased, while the expression level of superoxide dismutase was decreased dramatical y in the model group and experimental group (P<0.05);compared with the model group, the renal biochemical indicators, malondialdehyde and protein carbonyl contents were dropped significantly in the experimental group, but the expression of superoxide dismutase was significantly rebounded (P<0.05). Taken together, embryonic stem cel s can reverse the occurrence and development of diabetic nephropathy by inhibiting oxidative stress in progress.

Objective To study the significance of serum human epididymis protein 4 ( HE4 ) and human cartilage glycoprotein-39(YKL-40) in the diagnosis of ovarian cancer .Methods 100 patients with ovarian cancer , 90 patients with benign tumor of ovary and 50 healthy people were enrolled .Serum HE4 and YKL-40 were detected . Results The positive rates of serum HE 4 in ovarian cancer group , ovarian benign tumor group and healthy group were 86.0%,5.5%,2.0%.The positive rate of serum HE4 in ovarian carcinoma was significantly higher than that in benign ovarian tumor group and the healthy group (χ2 =31.06,30.82,all P<0.01).The positive rates of serum YKL-40 in ovarian cancer group ,ovarian benign tumor group and healthy group were 82.0%,3.3%,2.0%.The positive rate of serum YKL-40 in ovarian carcinoma was significantly higher than that in benign ovarian tumor group and the healthy group(χ2 =30.92,32.06,all P<0.01).Serum HE4 in the diagnosis of ovarian cancer ,the sensitivity was 86.0%,specificity of 95.7%,accuracy of 91.7%.Serum YKL-40 in the diagnosis of ovarian cancer ,the sensitivity was 82.0%,specificity of 97.1%,accuracy of 90.8%.For the joint detection,the sensitivity for diagnosis of ovarian cancer was 92.0%,and the specificity was 93.6%,accuracy was 95.0%,higher than those of the single detection . Conclusion Combined with serum HE 4 and YKL-40 detection can significantly improve the diagnostic accuracy of ovarian cancer patients .

ObjectiveTo study the optimized security strategy for informing cancer patients with the disease,and let patients be fully informed and even more actively cooperate with doctors while they are under treatment.MethodsGrouped with adversity quotient (AQ) score,these cancer patients were in comparison with those who had been informed by other ways without support strategy,and different groups of patients were estimated in different strategies in accordance with self-rating anxiety scale (SAS) and self-rating depression scale (SDS).Results With certain strategies,high and low AQ score groups' SAS and SDS scores stably restored in 1 day to 1 week,and the differences had statistical significance(P ＜ 0.01 ).The patients were well-informed without any difficulty and actively cooperated with treatment.No related factors led to self-mutilation,suicide and other serious incidents.While the SAS and SDS scores of the control group who were not informed by the strategy were not satisfactorily recovered,and the patients could not actively cooperate with the doctors.Especially being notified after 1 d to 1 week,the SAS and SDS scores had no significant improvement,and the differences had no statistical significance compared with themselves ( P ＞ 0.05 ).It is difficult to avoid the occurrence of serious incidents.Conclusion Based on AQ score,it is safe and effective to inform cancer patients of their disease condition by individual strategies.It not only respects patients rights,but also gains the active cooperation of the patients,and is helpful for treatment with minimizing adverse effects.

Objective To explore the characteristics, related risk factors and prognosis of cervical carcinoma in elderly women. Methods 163 cases with stage Ⅰ-Ⅱ cervical carcinoma according to International Federation of Gynecology and Obstetrics (FIGO) criteria underwent radical hysterectomy and pelvic lymph dissection from January 1997 to December 2006. They were divided into aged group, adult group and young group. The clinical pathological data, prognosis and recurrence were retrospectively analyzed. Results The prevalence rate of stage Ⅱ cervical carcinoma was 78.7% (37/47) in aged group, 61.7 % (58/94) in adult group and 59.1% (13/22) in young group. The prevalence difference was statically significant. The prevalence rate of non-squamous cell carcinoma was 8. 5 % (4/47) in aged group, which was higher than that in adult group [5.3%(5/94)]and lower than that in young group [18. 2% (4/22)] (P<0.05). In aged group, pathological grading were 78.7% (37/47) of middle differentiation and 4. 3% (2/47) of low differentiation(P<0. 01). The rate of lymph node metastasis was 8. 51% (4/47)in aged women, 27.7 % (26/94) in adult women and 40.90% (9/22) in young women (P<0. 01). The recurrence rate was similar among the three groups. The death rate was 85.7% in overall recurrent cases, (12/14)in aged group, 73.1% (19/26)in adult group and 50.0% (4/8)in young group, and there was no significant difference (P=0. 193). Conclusions Cervical carcinoma in elderly women has a later clinical stage, higher prevalence of non-squamous cell carcinoma, more middle differentiation tumour tissues and fewer lymphatic metastasis, which can provide evidence for clinical diagnosis and treatment.

Free radical plays a key role in the process of cerebral ischemic injury. It aggravates cell membrane damage through the peroxidation of unsaturated fatty acids, and then results in neuronal death and brain edema. Edaravone, a novel potent free radical scavenger, exerts its neuroprotective effect by inhibiting endothelial injury in cerebral ischemic areas and relieving neuronal damage. It also reduces thrombolytic therapy-induced brain edema and bleeding events after reperfusion. Clinical experiences suggest that the therapeutic time window of edaravone is very wide, and when it combines with thrombolytic therapy, it may reduce stroke mortality and promote the recovery of neurological deficit. This article reviews the course of development of edaravone from laboratory to clinical stage.

Objective To investigate the expression of Human leucocyte antigen(HLA)-DP,-DQ,-DR and CD40 in human retinal pigment epithelial(RPE)cells,to determine their molecule expression in immune response process,and their abilities to stimulate T lymphocyte activation. Methods Human RPE cells were cultured with or without(-IFN respectively.Expression of HLA-DP,-DQ,-DR and CD40 was measured by immunohistochemical staining.Meanwhile,peripheral blood mononuelear cells(PBMC)were co-cultured with RPE cells in vitro,and then the expression of activated lymphocytes CD69 was measured by fluorescence activated eell sorter(FACS). Results Expression of HLA-DP,-DQ,-DR and CD40 antigen were enhanced by γ-interferon inducement.Increasing amount of CD69 positive Iymphocytes were found in the co-culture system of RPE cells and PBMC. Conclusion T-lymphocytes in the peripheral blood were activated by human RPE cells which is antigen presenting cells with immunological characteristics potential.

Essential thrombocythemia (ET) which is a clonal disease of hematopoietic stem cell mainly reflects hyperplasia of megakaryocyte. Childhood ET is characterized by persistance of thrombocytosis, mild to moderate hepatomegalia and splenomegalia, thrombogenesis and hemorrhage complications. The pathogenesis of ET is obviously associated with the abnormal or disturbance expression of multiple genes, micro RNAs,immunological molecules and some chromosome aberration. This review will focus on recent developments in the pathogenesis of ET in molecular biology, immunology and cytogenetics.

Objective To establish a HPLC method for the determination of emodin in Dabaidu capsule. Methods The chromatographic system was adopted C18 column, using methanol -0.1% phosphoric acid (70∶30) as mobile phase. The content was detected at a waveleng of 254 nm and the flow rate was 1.0 ml/min.Results The emodin have a good tincar rolatim in the range of 6.181 ～ 18.54 μg/ml (r=0.9999), the average recovery rate was 98.93% and RSD = 0.65% (n = 6) . Conclusion The established method is accuracy,sensitive and simple.