BACKGROUND:Occurrence and development of diabetic complications is closely related to the severity of oxidative stress imbalance in the body. OBJECTIVE:To investigate the effect of embryonic stem cel s on oxidative stress response of rats with diabetic nephropathy. METHODS:Primarily cultured rat embryonic stem cel s were observed for cel morphology and surface antigen detection. Sprague-Dawley rats were divided into experimental group (two injections of embryonic stem cel s via the tail vein), model group (injection of the same volume of PBS), and normal control group (with no modeling, intraperitoneal injection of sodium citrate-citrate buffer). In the former two groups, the rats were intraperitoneal y injected sodium citrate-citrate buffer diluted streptozotocin to establish diabetic nephropathy models before treatment. At 5 weeks after the last injection, blood glucose level, renal function indicators (urine protein/urine creatinine, blood urea nitrogen and serum creatinine) were tested in each group;contents of malondialdehyde and protein carbonyl were detected in the kidney;the expression level of superoxide dismutase was detected by western blot assay. RESULTS AND CONCLUSION:The embryonic stem cel s were oval or round, with clear boundary and good refraction, and highly expressed Oct-4 and SSEA-1. Compared with the control group, renal biochemical indicators, malondialdehyde and protein carbonyl contents were significantly increased, while the expression level of superoxide dismutase was decreased dramatical y in the model group and experimental group (P<0.05);compared with the model group, the renal biochemical indicators, malondialdehyde and protein carbonyl contents were dropped significantly in the experimental group, but the expression of superoxide dismutase was significantly rebounded (P<0.05). Taken together, embryonic stem cel s can reverse the occurrence and development of diabetic nephropathy by inhibiting oxidative stress in progress.

ObjectiveTo study the optimized security strategy for informing cancer patients with the disease,and let patients be fully informed and even more actively cooperate with doctors while they are under treatment.MethodsGrouped with adversity quotient (AQ) score,these cancer patients were in comparison with those who had been informed by other ways without support strategy,and different groups of patients were estimated in different strategies in accordance with self-rating anxiety scale (SAS) and self-rating depression scale (SDS).Results With certain strategies,high and low AQ score groups' SAS and SDS scores stably restored in 1 day to 1 week,and the differences had statistical significance(P ＜ 0.01 ).The patients were well-informed without any difficulty and actively cooperated with treatment.No related factors led to self-mutilation,suicide and other serious incidents.While the SAS and SDS scores of the control group who were not informed by the strategy were not satisfactorily recovered,and the patients could not actively cooperate with the doctors.Especially being notified after 1 d to 1 week,the SAS and SDS scores had no significant improvement,and the differences had no statistical significance compared with themselves ( P ＞ 0.05 ).It is difficult to avoid the occurrence of serious incidents.Conclusion Based on AQ score,it is safe and effective to inform cancer patients of their disease condition by individual strategies.It not only respects patients rights,but also gains the active cooperation of the patients,and is helpful for treatment with minimizing adverse effects.

Objective To study the significance of serum human epididymis protein 4 ( HE4 ) and human cartilage glycoprotein-39(YKL-40) in the diagnosis of ovarian cancer .Methods 100 patients with ovarian cancer , 90 patients with benign tumor of ovary and 50 healthy people were enrolled .Serum HE4 and YKL-40 were detected . Results The positive rates of serum HE 4 in ovarian cancer group , ovarian benign tumor group and healthy group were 86.0%,5.5%,2.0%.The positive rate of serum HE4 in ovarian carcinoma was significantly higher than that in benign ovarian tumor group and the healthy group (χ2 =31.06,30.82,all P<0.01).The positive rates of serum YKL-40 in ovarian cancer group ,ovarian benign tumor group and healthy group were 82.0%,3.3%,2.0%.The positive rate of serum YKL-40 in ovarian carcinoma was significantly higher than that in benign ovarian tumor group and the healthy group(χ2 =30.92,32.06,all P<0.01).Serum HE4 in the diagnosis of ovarian cancer ,the sensitivity was 86.0%,specificity of 95.7%,accuracy of 91.7%.Serum YKL-40 in the diagnosis of ovarian cancer ,the sensitivity was 82.0%,specificity of 97.1%,accuracy of 90.8%.For the joint detection,the sensitivity for diagnosis of ovarian cancer was 92.0%,and the specificity was 93.6%,accuracy was 95.0%,higher than those of the single detection . Conclusion Combined with serum HE 4 and YKL-40 detection can significantly improve the diagnostic accuracy of ovarian cancer patients .

Cardiovascular Diseases ; etiology ; Chronic Disease ; Humans ; Hypertrophy, Left Ventricular ; etiology ; Kidney Diseases ; complications ; Risk Factors

Gastroenterology ; Humans

Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous group of non-Hodgkin's lymphoma.An uncommon subset with myc and either bcl-2 or bcl-6 rearrangement,also known as ‘double-hit’ lymphomas,is considered very aggressive clinical course and poor prognosis despite high-intensity chemotherapy.Recently,these lymphomas have received increased attention.This review explores the existing literatures for the involved genes with their functions,clinical features,diagnosis and treatment.

ObjectiveTo investigate and compare the efficacies of fire needling and warm needling in treating tenosynovitis of long head of biceps brachii.MethodNinety patients were randomly allocated to treatment and control groups, 45 cases each. The treatment group received fire needling and the control group, warm needling. The therapeutic effects were compared between the two groups. The difference in the shoulder pain and disability index (SPADI) score between before and after two or four weeks of treatment was assessed in the two groups.ResultThe total efficacy rate was 97.8% in the treatment group and 86.0% in the control group. There was a statistically significant difference in therapeutic effect between the two groups (P<0.05). The therapeutic effect was better in the treatment group than in the control group. In the two groups, the SPADI subscores were closely related to treatment time. There was a statistically significant difference between various time points (P<0.01). There were also statistically significant between-group differences in the SPADI pain score and total score (P<0.05).ConclusionBoth fire needling and warm needling are effective in treating tenosynovitis of long head of biceps brachii. The therapeuticeffect of fire needling is superior to that of warm needling.

Objective To investigate the influence of trimetazidine combined with atorvastatin on heart func-tion and the BNP level in patients with cornary heart failure.Methods The clinical data of 400 patients with cornary heart disease were retrospectively analyzed.194 patients in the control group were treated with conventional treatment, 206 patients in the observation group were treated with trimetazidine combined with atorvastatin on the basis of the control group.The treatment effect,cardiac function and BNP level were observed and compared.Results The effec-tive rate of the observation group was 91.26%,which was significantly higer than 76.29% of the control group (χ2 =16.667,P <0.05).After treatment,the cardiac index,stroke volume,cardiac output,left ventricular ejection fraction and BNP level of the two groups were significantly higher than before treatment,and those in the observation group were significantly higher than the control group(t =2.505,2.851,3.308,3.452,all P <0.05).Conclusion Trime-tazidine combined with atorvastatin in treatment of cornary heart failure has good curative effect,it can reduce the serum level of BNP,improve cardiac function significantly,which is of great value for clinical use.

Objective To investigate clinical efficacy and safety of metformin combined with sitagliptin or pioglitazone in treatment of type 2 diabetes mellitus (T2DM).Methods Senvnty-six patients with T2DM hospitalized from January 2011 to December 2012 were randomly divided into A(n =40) and B (n =36) group.Patients in group A were given metformin plus sitagliptin scheme for 12 weeks,and in group B were given metformin plus pioglitazone scheme for 12 weeks.The blood glucose level was measured and clinical efficacy and adverse drug reactions during treatment were record.Results (1) Fasting plasma glucose (FPG),2 h postprandial blood glucose(2 hPBG) and glycosylated hemoglobin(GH) in A and B group after 12 weeks of treatment were (6.4 ± 1.8) mmol/L,(7.0 ± 1.9) mmol/L; (8.1 ± 2.7) mmol/L,(9.5 ± 2.8) mmol/L; (6.2 ± 2.1) ％,(7.1 ± 2.2) ％,lower than that of pre-treatment ((9.4 ± 2.4) mmol/L,(9.1 ± 2.5) mmol/L; (13.6 ± 4.5) mmol/L,(13.3 ± 4.9) mmol/L; (9.2 ± 2.4) ％,(8.9 ± 2.6) ％ and the difference were significant (t =6.325,4.013,6.628,4.040,5.950,3.171 respectively,P ＜ 0.05).2 hPBG level of group A was (8.1 ±2.7) mmol/L,lower than that of group B ((9.5 ±2.8) mmol/L,t =2.214,P =0.030).(2) Efficiency in group A was 90.0％ (36/40),slightly higher than that of group B (83.3％ (30/36)),but the difference was not statistically significance (x2 =0.269,P =0.604).(3) There were no seriously adverse drug reactions in two groups.Rates of adverse reactions in two groups were 25.0％ (10 cases),30.6％ (11 cases) respectively (x2 =0.292,P =0.589).Conclusion Both treatment plans were proved effective in treatment of patients with T2DM.Compared with plan of metformin plus pioglitazone,metformin plus sitagliptin was showed a comparative advantage in clinical efficacy,especially with outstanding curative effect in reducing postprandial blood glucose.