BackgroundIn 1988, the Forty-first World Health Assembly adopted a resolution for the Global Polio Eradication.Since the initiative was launched, number of polio cases has fallen by over 99%. Today, only threecountries in the world, Afghanistan, Pakistan and Nigeria - remain polio-endemic. The Polio Eradicationand Endgame Strategic Plan of 2013-2018 calls for the gradual eradication of wild virus strain and thevaccine virus strain at the same time. In order to prevent the border transmission of wild type poliovirus,virus leakage from laboratories, it is required to conduct inventory of laboratories handling poliovirus andpotential infectious materials every 2 year.GoalTo identify laboratories handling poliovirus and potential infectious materialsMethodsSurvey of laboratories handling poliovirus and potential infectious materials was conducted amongstate, private, clinical, biomedical and environmental testing in total of 127 laboratories operating in21 provinces and 9 districts of Ulaanbaatar city by questionnaire. Survey questionnaire consists of 6sections (general, sample storage, laboratory biosafety, staff knowledge, information source, trainingand etc.). Study results were processed using SPSS-19 statistical programme.Results34.7% of 96 biomedical laboratories were analyzed stool samples. These laboratories were analyzedrotavirus (17.0%), intestinal bacteria (67.0%), Helicobacter (14.3%), parasite and other indicators (1.7%)in stool samples. 43.8% of laboratories were stored stool samples for one day and 3.1% up to oneyear. From 31 environmental testing laboratories 73.3% were bacterialdetection test on environmentalsamples. 60% of wastewater samples were collected from rivers, 16% on entrance to wastewatertreatment plant and after biological treatment combined, and 24% from other sources. Soil sampleswere collected near waste disposal and other sources (46.4%), and from unknown sources (53.6%).24.1% of all laboratories were stored environmental samples for 3 days, 3.4% for 45 days. Accordingto results, surveyed laboratories did not store samples for more than 1 year. Also, none of surveyedlaboratories (100%) were not stored poliovirus and potential infectious materials.Conclusion· The investigated laboratories were not stored poliovirus and potential infectious materials.· The biosafety and biosecurity status of laboratories should be improved in near future throughenhancing knowledge of laboratory workers and organizing training related to biosafetyandbiosecurity.

BACKGROUND: Acute cholecystitis is defi ned mostly as bacteria from intestinal infl ammation to gallbladder. Sometimes the inflammation can occur when bacteria and viral can fl ow by blood and lymphus. Acute cholecystitis is leading the second place of acute abdomen. (1.2.3.7.8). The acute cholecystitis complication is not decreasing(4.5.6). The mortality is 0.5-0.8%(2.4.5.9). The acute cholecystitis is comparing with cholelith. Foreign scientists are recommending that fi rst 24-48 hours to treat by drugs, and after that if infl ammation is not healing to do cholecystectomy. In our country the acute cholecystitis is taking the place after acute abdomen and appendicitis. And also, acute cholecystitis morbidity is not decreasing and indication of cholecystectomy is not decided yet. OBJECTIVE: The main purpose of this survey is to study changing of acute cholecystitis depending on time Materials and Methods We studied 58 patients who had cholecystectomy in Surgical Department of The Central Clinical Hospital for State Special Clerks between 2005 and 2008. The result analyzed by SPSS-15.0 Program. RESULT AND DISCUSSION: The patients who was studied were 14 men (24.14%) and 44 women (75.86%). For the clinical symptoms of acute cholecystitis, the result has been occurred as following: the epigastria pain is 17 (29.31±5.9), around the right rib arch is 49 (84.48±4.7), the pain spread of the right shoulder blade is 20 (34.48±6.2), and the pain spread of the right shoulder is 33 (56.89±6.5), to have a fever 6 (10.34±3.0), vomit 10 (17.24±4.9), diarrhea 7 (12.06±4.2), thirsty 16 (27.58±5.8). The pain around right rib arch, pain spread right arm and shoulder, and thirsty are the clinical features that close to the features of scientist’s Alperovich B.I., Soloviev M.M., Saveliev V.S. Acute cholecystitis depending on time 0-24 hours catarrhal 5, phlegmonous 2, necrosis 1, 24-48 hours phlegmonous 4, necrosis 10, necrosis hole 2, 48-72 hours phlegmonous 10, necrosis 8, necrosis hole 3, above 72 hours phlegmonous 2, necrosis 5, necrosis hole 6. Acute cholecystitis starts above 24 hours. CONCLUSION: 1. The acute cholecystitis has been occurred 14 for men and 44 for women. Ate the age of 30-39. These cases were determined more then 31 percent. 2. For the clinical symptoms of acute cholesystitis, the result has been occurred as following: - the epigastria pain is 17, (29.31±5.9) - around the right rib arch is 49 (84.48±4.7) - the pain spread of the right shoulder blade is 20 (34.48±6.2), and - the pain spread of the right shoulder is 33 (56.89±6.5). 3. Under the period study of the acute cholecystitis, the pus, necrosis and perforation cases have been excessively occurred specially at 48-72 hours. 4. Under the comparison study between the acute cholecystitis and its period, the acute wall cholecystitis changes have been occurred specially at 24-48 hours.

Abstract Mana-4, an herbal medicine, had been used to treat incomplete-mannered and infection-caused hot disease in Mongolian traditional medicine. It has already reported that Mana-4 acts as an anti-inflammation agent, an activator of T and B cells, an immune-modulator and an inducer of cellular proliferation. Moreover, it enhances the immune system and energy level of human body. It was confirmed that the main active compounds in Mana-4 are inulin and total flavonoids which are effective for many diseases. Drug formulation types are very important to delivery the drugs to the targeted tissues and organs without loss of active ingredients. Total flavonoids in the extract of Mana-4 and granulated Mana-4 was qualitatively evaluated by TLC and yellow-brown spots (Rf was 0.4) were found on TLC plates, indicating that the preparations contained flavonoids. Also, it confirmed that the appropriate extractor of total flavonoids from Mana-4 was 70% of ethanol. In conclusion, the tablet formulation from Mana-4 was successfully prepared and the quality requirements was allowable.

Background: Infections in respiratory systems have spread throughout the world without any restrictions including living places, public issues, and lifestyle. Three main causes of illnesses for the population of cities and rural areas were gastrointestinal diseases, respiratory diseases, and cardiovascular diseases. After investigated some medicinal herbs including Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa, it has been reported that they had antiinflammatory, analgesic, and wound healing effects. Lozenge formulation has some advantages for treatment application, such as easily absorbed, good bioavailability and ability of diminishing stomach irritation. In this study, we aimed to obtain a suitable extract from Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa for further lozenge formulation. Purpose: To obtain a suitable extract from Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa, and to conduct qualitative and quantitative studies for some biologically active substances Materials and methods: In this study, an aerial part of Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa were used, and the study was conducted in MUPS. For obtaining a suitable extract, the raw materials were extracted by remaceration, repercolation and circulation methods in 20% and 70% of ethanol and distilled water. The flavonoids and polyphenolic compounds in the extracts were determined by thin layer chromatography. Quantitative analysis for total flavonoids was performed by spectrophotometer. Results: According to the result, a yellow spot-on chromatogram was detected in extracted raw materials (Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa), indicating that flavonoid contained in the extracted solution.
The result was compared to standards of rutin (Rf=0.2) and quercetin (Rf= 0.94). Also, a black, blue spot-on chromatogram was detected in extracted raw materials (Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa), indicating that polyphenols contained in the extracted solution. The spots were compared to gallic acid as a standard substance. In the quantitative assay of total flavonoids in raw materials, black-green precipitation was revealed after procedure. From this result, remaceration and circulation techniques were suitable to extract the raw materials. Flavonoid content was 3.35±0.04% after using remaceration technique, which indicated that it was more suitable to extract the raw materials. Conclusions These results showed that the appropriate extracting solution for Stelleria Chamaejasme L. and Oxytropis Pseudoglandulosa was 70% of ethanol. In this case, 3.35±0.04% of flavonoid was extracted by remaceration technique.