Aims: The use of herbicide effectively controls weeds in agricultural practice. However, its release to the surrounding surface water bodies may lead to environmental issues. The aim of this study was to isolate the bacteria that were able to remove 2,2-dichloropropionic acid (2,2-DCP) from a paddy field located in Malang. Methodology and results: The 2,2-DCP degrading bacteria were isolated and their ability to grow on higher 2,2-DCP concentrations (50 and 80 mM) was tested. Bacterial degradation of 2,2-DCP was examined through measurement of released chloride ions. The potential isolates were identified according to their 16S rDNA sequences. Two potential isolates, BB9.2 and BC14.3 were observed for their growth on 20, 50, and 80 mM 2,2-DCP. Isolate BC14.3 had the shortest cell doubling time of approximately 4.1 h with 100% 2,2-DCP (20 mM) utilization, whereas BB9.2 was only able to degrade 80% of 2,2-DCP at the same concentration. The 16S rDNA gene sequences suggested that BB9.2 and BC14.3 belong to Acinetobacter calcoaceticus and Pseudomonas plecoglossicida, respectively. Conclusion, significance and impact of study Bacterial strains with 2,2-DCP degrading potentials were successfully isolated from long-term exposed agricultural soil. They demonstrated notable utilization of the organic halide. This is the first time that strains of A. calcoaceticus and P. plecoglossicida were reported to utilize 2,2-DCP.

Aims: This study presents the first structural model and proposed the identity of four important key amino acid residues, Asp13, Arg51, Ser131 and Asp207 for the stereospecific haloalkanoic acid dehalogenase from Rhizobium sp. RC1. Methodology and results: The enzyme was built using a homology modeling technique; the structure of crystallized LDEX YL from Pseudomonas sp. strain YL as a template. Model validation was performed using PROCHECK to generate the Ramachandran plot. The results showed 80.4% of its residues were located in the most favoured regions suggested that the model is acceptable. Molecular dynamics simulation of the model protein was performed in water for 10 nanoseconds in which Na+ was added to neutralize the negative charge and achieved energy minimization. The energy value and RMSD fluctuation of Cα backbone of the model were computed and confirmed the stability of the model protein. Conclusion, significance and impact of study: In silico or computationally based function prediction is important to complement with future empirical approaches. L-haloacid dehalogenase (DehL), previously isolated from Rhizobium sp. RC1 was known to degrade halogenated environmental pollutants. However, its structure and functions are still unknown. This structural information of DehL provides insights for future work in the rational design of stereospecific haloalkanoic acid dehalogenases.

Halogenated compounds create the most important class of xenobiotic which commonly lead to pollution. Some of these compounds are very toxic and cause enormous problems to human health and to the environment. Many of these toxic chemicals have been shown to occur in various extreme habitats. Pollutant-degrading microorganisms, adapted to grow in various environments, play an important role in the biological treatment of polluted extreme habitats. The presence of dehalogenase producing microorganisms in extreme habitat in particular is necessary since the enzyme can catalyze the removal of a halogen atom from a substrate. Therefore, it can reduce the toxicity of the halogenated compound and some are of interest for study in industrial application. Thermophiles, psychrophiles, acidophiles, alkaliphiles and halophiles are types of extremophiles. Knowledge of the biodegradation of toxic chemicals in extreme environment is limited. Here, examples of dehalogenase producing bacteria isolated from various extreme conditions and its special characteristics/features will be discussed in this review.

BACKGROUND: A student enrolled in Allied Health Sciences has a curriculum and academic standards that are different compared to a student enrolled in a medical programme. Nevertheless, little is known about the psychological distress of this population. Our objective was to determine whether psychological distress is prevalent in students enrolled in an Allied Health Sciences programme, as well as factors that contribute to it. METHODS: Three hundred and thirty-three (333) undergraduates studying Allied Health Sciences, ages 18-30, were included in this cross-sectional study. A representative sample of students from nursing, physiotherapy, medical laboratory science, medical radiography, and optometry programmes were selected through a stratified sampling technique. A 21-item Depression Anxiety and Stress Scale (DASS-21  10) was used to collect data on psychological distress indicated by depression, anxiety, and stress. We fitted a multivariate logistic regression to identify factors associated with psychological distress among the participants. RESULTS: As a result of the study, 58.9% reported depression, 66.1% reported anxiety, and 45.1% reported stress. Depression (AOR= 3.23; 95% CI: 1.33- 7.81), anxiety (AOR= 3.33;95% CI:1.34- 8.26) and stress (AOR= 2.58;95% CI: 1.14- 5.85) were associated with family history of mental illness. Poor academic performance was associated with anxiety (OR=2.75; 95% CI: 1.35- 5.61) and stress (OR=2.00; 95% CI: 1.05-3.81). CONCLUSIONS The prevalence of psychological distress among allied health sciences students is high, especially among students with poor academic performance and those with a family history of mental illness. Psychological distress can be prevented with early detection and awareness programmes.
Depression ; Anxiety

BACKGROUND: Research is the act of inquiry to know and is one of the pivotal points and fundamental goals of a university. It is an important part of an academic job and is believed to be the next most valued part of their duty after teaching OBJECTIVES: This study aims to determine the research productivity of Nigerian academics in medicine and allied health sciences disciplines in Nigeria. METHODOLOGY: 177 participants completed a 41- items questionnaire that elicits information on sociodemographic, the institution, the program, and research productivity (research papers, conferences attendance and presentation, and book chapters) using a multistage cluster random sampling of Nigerian Universities. RESULTS: Majority of the included participants were male (70.06%), married (79.14%), and below the senior teaching cadre (38.99%). Majority of the academicians do not have any training overseas (67.06%) and have indicated time as a constraint to research productivity (67.06%). Three papers in the past two years is an indication that Nigerian academics in the medical and allied health sciences, on average, may not be deemed to be highly productive. Professors (16.58+27.14) and those in higher ranks (Readers: 11.84+11.22 and senior lectures 12.58+13.53) have published more papers than their counterparts in the junior cadre (lecturer I and II: 4.87+6.58 and 2.69+3.87). Academics in the present study has a median research grant of zero in the past two years. A simple majority of the participants (55.29%) agree on mandating of PhD for promotion and career advancement of academics. There is no difference in the number of papers published (6.04+10.52 and 7.62+13.21) or conferences attended (7.42+19.14 and 4.04 +4.02) between academics in private and public Universities CONCLUSION Apparently low and dismal productivity by these academics in medicine and allied health sciences affirm the need for capacity building on an ongoing basis.

BACKGROUND: Pregnancy-related low back pain is a common complaint with numerous adverse consequences. Unfortunately, the effect of healthrelated quality of life and physical activity status on pregnancy-related low back pain remains understudied. OBJECTIVES: This study aimed to examine the influence of physical activity and health-related quality of life on pregnancy-related low back pain among pregnant women. METHODS: A survey of 398 pregnant women was conducted using the Modified Pregnancy Low Back Pain, Medical Outcome Survey Short Form (MOS-SF), and Pregnancy Physical Activity Questionnaire (PPAQ) to assess pregnancy-related low back pain, quality of life, and physical activity status among the participants. RESULTS: About fifty-four percent (54%) of the pregnant women reported experiencing low back pain during pregnancy. There were no significant differences between most physical activity intensities and domains for pregnant women with and without pregnancy-related low back pain, with the exception of women identified as sedentary (12.9±14.8 versus 17.4±16.2) or inactive (18.7±20.0 versus 23.6±19.8). Furthermore, waist circumference (OR: 0.97; 95% CI: 0.96 to 0.99) and Physical component scores (PCS) (OR: 0.96; 95% CI: 0.93 to 0.98) were associated with pregnancy-related low back pain. An interaction of height and occupation also showed an association with pregnancy related low back pain (OR: 1.04; 95% CI: 1.01 to 1.08). CONCLUSION Sedentary and inactivity are the only physical activity intensity and domain associated with pregnancy-related low back pain, which affect the physical well-being of the expecting mothers.
Exercise