Objective: The Beagle dog is suitable for biomedical researches due to its stable biological characteristics.This study aimed to investigate the anatomical application value of the Beagle dog in the reconstruction of hypopharyngeal and cervical esophagus.Methods: Ten Beagle dogs selected for the reconstruction of hypopharyngeal and cercvical esophagus with free jejunal flap transplantation underwent detailed local anatomy of the neck muscles,blood vessels and nerves.Results: The Beagle dogs had strong neck muscles,superficial and thin jugular veins,clear arterial branches,firm esophagus endomembrane,and strong antitrauma capacity.Conclusion: Anatomic characteristics of the neck of the Beagle dog has special application value and clinical significance for further studies on neck tissue defect reconstruction and organ transplantation.