Introduction: Dengue fever is one of the world’s most severe mosquito-borne diseases, with high morbidity and mortality rate. Poor dengue prevention practices are one of the factors contributing to dengue outbreak. National Health and Morbidity Survey (NHMS) have collected lots of population data on the prevalence of various non-communicable and communicable diseases, including dengue. NHMS 2020 however, focus on communicable diseases i.e., Dengue, Malaria, HIV & Covid-19. Notwithstanding, data on Cognitive, Affective and Behaviour (CAB) domains are scarce. Thus, this study was aimed to develop and validate CAB questionnaire on dengue in Malaysia. Material and Methods: The questionnaire was developed by adapting and improvising similar previous questionnaires used during NHMS 2015, selected published questionnaires, expert panel and literature review. A sample of 200 respondents was used to evaluate the internal consistency of the final instrument. Results: 5 experts reviewed the content validity of the CAB questionnaire while 20 respondents tested its face validity. The Cronbach’s alpha coefficients scores were within the acceptable range from 0.661 to 0.677. Various adjustments were made by deleting items with low Cronbach alpha values in attempt to attain the highest possible alpha values, but further deduction could not be made due to the retention of several important domain-measurement items. Conclusions: The validity and reliability of the CAB-IHBR-Dengue-A-01 questionnaire were of ‘acceptable’ ranges and can be used to assess CAB. The questionnaire is a valid and reliable tool for assessing the CAB’s ability to influence dengue prevention among Malaysians.