The result of a nutritional survey on potassium intakes of population and serum potassium contents of healthy adults in Shanghai (high occurrence of hypokalemia), Sichun (moderate occurrence of hypokalemia) and Shandong (null occurrence of hypokalemia) revealed that the average daily potassium intakes for the three areas were 60.1?16.6, 54.6? 13.4 and 58.7? 11.1 mmol per capita per day respectively; The serum potassium contents of the three areas were 4.2?0.3,4.0 ?0.3 and 4.2?0.4 mmol/L respectively. These results indicated that the daily potassium intakes and serum potassium contents between Shanghai and Shandong were not significantly different and might suggest that the occurrence of gossypol related hypokalemia in Shanghai and Sichun could not be attributed to the low potassium intakes and low serum potassium contents of the population.

Iliac arteriovenous ifstula (AVF) usually manifests in a wide range of symptoms similar to typical deep venous thrombosis (DVT), which often lead to delayed diagnosis or misdiagnosis. We reported a 51-year old woman who was performed lumbar discectomy and showed a progressive abdominal distention, dyspnea, and swollen left leg. She was initially diagnosed as deep vein thrombosis and the ifnal diagnosis was arteriovenous ifstula. hTe ifstula was successfully sealed by an endovascular covered stent. No further recurrence was found atfer a half year’s follow-up. hTis article summarized the experience regarding iliac arteriovenous ifstula misdiagnosed, and discussed the differential diagnosis between arteriovenous ifstula and pulmonary thromboembolism causedby deep vein thrombosis.

In the present study, packaging system composed of pAAV-CMV-GFP, pAAV-RC and pHelper were transfected into human embryonic kidney 293 cells (HEK293 cells) mediated by polyethyleneimine (PEI) to explore an optimal transfection condition. Different total plasmid DNA dosages (1, 2, 3, 4, 5, 6 μg) and different PEI/Plasmid ratios (1:1, 3:1, 5:1, 7:1) were tested with detection of green fluorescence protein (GFP) with ImagePro Plus6. 0 Software. Then transfection efficiency of the optimized transfection system was further observed for different time periods(12, 24, 36, 48, 60, 72 h). The results showed that total plasmid dosage of 4 μg/well with PEI/plasmid ratio of 3 : 1-5 : 1 was an efficient transfection condition. Transfection efficiency-time curve was an S-shaped curve. Transfection efficiency reached a plateau at 60 h after transfection. The optimized conditions for PEI-mediated transfection at the optimal time result in enhanced transfection efficiency of triple plasmid into HEK293 cells.
Green Fluorescent Proteins ; HEK293 Cells ; Humans ; Plasmids ; Polyethyleneimine ; Transfection ; methods

1.The nutrient content of yeast (Condida Y-17) grown on n-alkane studied in this experiment was comparable to that of the same type of yeast produced in other countries. Its content of lead, arsenic, mercury and benzo (a) pyrene were within the limit proposed by the Protein-Calorie Advisory Group of the United Nations System. The Content of its residual n-alkanes and total aromatic hydrocarbons were within or a little over that limit. It is a good supplementary protein feed.2.The corrected PER values were, yeast grown on n-alkane 1.18; deli pid and denucleic acid yeast grown on n-alkane 1.16; brewer's yeast 1.52; full fat soybean meal 1.81. The PER of yeast grown on n-alkane was significantly lower than those of other samples. When 0.3% DL-methionine was added to the diet, the corrected PER values increased: n-alkane grown yeast 2.32; delipid and denucleic acid n-alkane grown yeast 2.49; brewer's yeast 2.35; full fat soybean meal 2.28. The corrected PER value of casein used for correction was 2.43, it was standardized with ANRC reference protein (PER = 2.50).3.Yeast grown on n-alkane with and without removal of lipids and nucleic acid was fed to groups of rats at a dietary level of 25% and 35% respectively for 1 year. A stock diet group and a brewer's yeast diet group were used as control. In the first 3 months the weight gain and feed consumption of the rats fed n-alkanc grown yeast diet were lower than those of the two control groups. The male rats were more sensitive to the change of diet. In the later 9 months the difference of weight gain among different groups were not significant. The concentration of haemoglobin, red cell counts, white cell counts, GPTase activity, serum cholesterol levels and serum urea levels determined at the beginning and the end of the experiment were all in the normal ranges. The concentrations of n-alkanes and odd-chain fatty acids in the adipose tissue of the n-alkane grown yeast group were higher than those of the two control groups, and the difference was highly significant. The concentrations of total aromatic hydrocarbons and benzo(a) pyrene in the muscle of different groups of rats were 0.4-0.6 ppm and 0.4 ppb respectively. No detrimental effect was observed in pathological examination.4.When 5% fish meal or 3.5% casein was added to the 25% n-alkane grown yeast diet, the weight gain and feed consumption of weanling rats were improved significantly, though it was still inferior to those fed with stock diet.5.Strongly growth depressive effect was noted as the level of n-alkane grown yeast was increased to 20% in the diet. This depressive effect was neither due to the toxicity of the residual n-alkanes and aromatic hydrocarbons, nor due to the deficiencies of potassium and selenium in the diet. It might be the result of nutrient or nutrients imbalance of the diet. The appropriate amount of yeast grown on n-alkane used in mixed feed should not be more than 15%.

A longitudinal study was carried out on fifty infants selected from both urban and rural areas of Beijing for their body weights, heights, head and mid-upper arm circumferences, tricep and abdomen skinfold thicknesses, and breatmilk and energy and protein intakes at the 1st, 3rd and 6th month of life. The results revealed that the breastmilk intakes of these infants were ranged from 600-800 g/day during this period of time, the breastmilk intakes of the rural infants were higher significantly than that of the urban infant throughout the study period (P