Objective To evaluate the biologic characteristics of a new type femoral stems either coated with gradient hydroxyapatite (HA) or additionally loaded with rhBMP-2. Methods Fifteen dogs were divided into 3 groups according to the implanted hip prosthesis. Each animal received hemiarthroplasty in the right hip with prosthesis made of pure Ti-6Al-4V femoral stem (Ti group), the new gradient HA-coated femoral stem (HA group) with 100 to 150 ?m hydroxyapatite coating, and the new gradient HA-coated femoral stem loaded with rhBMP-2 of 9 mg (BMP-HA group) respectively. The dogs were sacrificed at 12 weeks after operation, and labeled with tetracycline 3 of 30 mg/kg days prior to sacrifice, and then the histological changes of the tissue surrounding the prosthesis were evaluated. The radiographic examinations were taken before and after sacrifice. Results On radiographic examination, local radiolucent line around the prosthesis was observed only in one specimen in Ti group. The new bone formation was found both in HA group and BMP-HA group more obviously than in Ti group. Under microscopy, it was noted that this new gradient HA-coating was intact and no obvious biodegradation in most section, whereas a fibrous membrane was found in Ti group. The average rates of interface osteointergration in Ti group, HA group and BMP-HA group were 4.05%?7.66%, 71.04%?9.81% and 88.86%?6.56%, respectively, those of HA and BMP-HA group were significantly higher than that of Ti group (P

Objective: To study the expressions of CD147and MMP9 in the lung metastasis model of breast cancer in BCML-TAII99 mice and their correlation with tumor growth and metastasis thereof. Methods: The breast cancer model with lung metastasis was established in BCML-TAII99 mice. The immunohistochemical staining method(SP method) was used to analyze the expressions of CD147 and MMP9 in mice lung metastasis tissue. The relationship between the tumor growth and the metastasis was analyzed. Results: The positive rates of CD147 and MMP9 were 63.33%（19/30）and 53.33%（16/30）respectively in mouse models. There was significant difference in positive rate of expressions between the metastasis group and the non-metastasis group（χ~2= 6.238， P ＝ 0．013； χ~2＝ ＝5．129， P ＝ 0．024). There was a statistically significant positive correlation between the expression of CD147 and MMP9（r = 0．786， P ＝ 0.025). Conclusion: The expression of CD147 may play a crucial role in infiltration and metastasis of breast cancer by inducing the expression of MMP9.

This article was aimed to study the Uygur medicine Hyssopus officinalis L. on the balance of Th1/Th2 and Th17/Treg of chronic obstructive pulmonary disease (COPD) mice in order to explore the mechanism of its treatment of COPD. The pre-fumigation method was copied to establish a mouse model of COPD. Mice were ran-domly divided into the normal control group, saline group and water extract of H. officinalis L. high, middle and low dose group. The level of serum IFN-λ, IL-4, IL-17 and IL-10 were detected with ELISA. The results showed that compared with the normal control group, the serum IL-4 and IL-17 level of the COPD model group were significantly higher (P < 0.05), and the serum IFN-λ and IL-10 level were significantly lower (P < 0.05). After drug intervention, the IL-4 and IL-17 level were significantly lower, and the IFN-λ and IL-10 level were significantly increased compared with the model group. The correlation analysis showed that the IFN-λ, IL-4, IL-17 and IL-10 were negatively related. It was concluded that H. officinalis L. played a certain role on correct-ing imbalances of Th1/Th2 and Th17/Treg.