Objective To analysis the influence of health management intervention on blood glucose control for 2 diabetic, discusses in view of the diabetic not good life style practice the effective health management measure. Methods Reviewed the diagnosised 2 diabetic examples (1152) from March,2003 to July,2007 by healthy physical examination and the physical examination materials, including the biochemistry items like fasting blood glucose (GLU), Glucosylated hemoglobin (HbAlc) and the questionnaires about each patient's formerly body activity, the diet and the medical compliance etc. Established the standard,carried on the appraisal. According to the above, the life style relative good 283 examples (A group) and the bad 869 examples (B group) the patient will integrate the cohort, carried on the cohort study, analysised the relations between life style and diabetes controll effectiveness; At the same time, compared the biochemistry results between the non-managed (C group) and the managed (D group) after by meanse of a year strengthened health management intervention (diet, movement, medical compliance, health education etc.) on the 241 example patients. Results The GLU and HbAlc control satisfaction rate of the life style relative good group's (A group) respectively is 60. 1% and 56. 5%, obviously surpasses the life style bad group's (B group) 49.0% and 45. 5% (X2 = 10.44 and 11.05, P<1.01) ; The unscientific life style is diabetes GLU and the HbAlc control not satisfactory the dangerous fact (RR = 1.28 and 1.25) ; After carries on the life style strengthening intervention for a year (D group), the nutrition and the movement knowledge awareness rate (63.5%) is obviously higher than the before management (C group) (25.7%), the difference has statistics significance (X2=4.51, P<0.05), after and before the strengthening life style management intervention, GLU and HbAic control satisfaction rate is respectively 61.8% ,64. 3% and 58.5%, 47.8%, the difference has statistics significance (X2=7.94 and 4.18, P<0.01 and 0.05) Conclusions The life style has a close correlation with the effectiveness of 2 diabetes blood glucose control, the health management intervention can raise the patient's good life style, helps the patient to obtain satisfaction the blood glucose control effectiveness.

Objective:To express fusion protein of mouse thymic stromal lymphopoietin (TSLP) and HIV-1 gp120BAL V1/V2 subdomain in 293F cell.Methods:Full length of the V1V2 sequence from BAL isolate was fused with the C-terminus of mouse thymic stromal lymphopoietin (TSLP) and sub-cloned into pCEP-Pu vector to construct the eukaryotic expression plasmid-pCTV1V2BAL.The recombinant plasmid was confirmed by enzyme digestion and sequencing , then transfected into 293 F cells using PEI as a transfection reagent .The fusion protein was purified by metal chelate affinity chromatography and characterized by SDS -PAGE and Western blot . The epitopes of V1/V2 in fusion protein were identified by ELISA .Results:The SDS-PAGE and Western blot results showed that there were highly heterogeneous glycoprotein bands at the site between 35 kD and 55 kD, which reacted with anti-mTSLP rabbit polyclonal antibody and anti-His tag mouse monoclonal antibody .The ELISA analysis showed that antibodies to V 1/V2BAL existed in the sera of HIV-1 positive patients.Conclusion:The mTSLP-V1/V2 fusion protein was successfully expressed in 293F cells, which may be useful for HIV-1 vaccine research .