OBJECTIVE To investigate and know the site distribution,susceptible factors and antibiotic resistant condition in hospital-acquired infection by Enterococcus so as to provide reference for clinical preventive control and for reasonable uses of medicine.METHODS Sixty five Enterococcus strains isolated from different infection specimens in the hospital from 2001 to 2005 were analyzed,and examined their sensitivity to medicines.RESULTS Among 65 Enterococcus strains there were 36 strains of Enterococcus faecalis and 29 strains of E.faecium.The first three susceptible site diseases were alimentary,circulatory and injury.The site distributions were urinary tract,lower respiratory tract and surgical incisions.Related aggressive operations included urethra intubation,tracheal intubation,and surgery.Except for vancomycin and teicoplanin,E.faecalis was sensitive to ampicillin,high-level streptomycin,levofloxacin and nitrofurantoin,and E.faecium had antibacterial activity to chloramphenicol and tetracycline.There were 2 vancomycin-resistant Enterococcus(VRE) cases detected.CONCLUSIONS It is the easiest to examine and find Enterococcus in urine and the prevalence rate is high after related aggressive operations.The isolation rate of E.faecium is high and it has strong antibiotic resistance.Vancomycin is still the most sensitive medicine to Enterococcus.