Objective To analyze the expression of secreted aspartyl proteinases (Sap) in human vaginal infection in vivo. Methods Vaginal swabs were collected from 9 healthy volunteers and 20 patients with vaginal candidiasis, and the expression of Sap1-6 was evaluated by reverse-transcriptase polymerase chain reaction using specific primer sets. Results The Sap2 and Sap5 were the most common genes expressed in the patients with vaginal candidiasis. The expression Sap3 and Sap4 was detected in all subjects. All six Sap genes were simultaneously expressed in some patients with vaginal candidiasis. Conclusion The data shows that the Sap genes may be involved in the pathogenesis of vaginal candidiasis.

We retrospectively reviewed clinical data of 150 elderly patients with serious chronic congestive heart failure who admitted in 1986, 1996 or 2006, including medication treatment, hospital stay,and admission interval. The results indicated that before 1990s, digitalis, diuretics, and blood vessel dilating agents were more often used in the treatment of chronic congestive heart failure(digitalis=100%,diuretics≥95%, blood vessel dilating agents>50%, β-blocker<10%, ACEI/ARB = 0% ). Since 1990s,the use of β-blocker, ACEI and ARB increased, which resulted in reduced hospital stay and increased admission interval(P<0.01). Our investigation suggests that evidence-based medication could be necessary in the treatment of elderly patients with chronic congestive heart failure.

In order to analyze the in vivo expression of Candida albicans secreted aspartyl proteinases (SAP) in human vaginal infection, the vaginal secretion from 29 human subjects was collected by vaginal swab, and the expression of SAP1-SAP6 was detected by reverse-transcriptase polymerase chain reaction using specific primer sets. It was found that Sap2 and Sap5 were the most common genes expressed during infection; Sap3 and Sap4 were detected in all subjects and all 6 SAP genes were simultaneously expressed in some patients with vaginal candidiasis. It was suggested that the SAP family is expressed by Candida albicans during infection in human and that Candida albicans infection is associated with the differential expression of individual SAP genes which may be involved in the pathogenesis of vaginal candidiasis.

Objective To explore the effects of partial body weight support treadmill training (PBWSTT) on motor function of lower extremities, walking ability and activities of daily living (ADL) in chronic hemiplegic patients after stroke. Methods28 patients with stable stroke (hemiplegic limbs Brunstrom s' scale≥Ⅲ) exceed 12 months after the onset were divided into PBWSTT group (n=14) and control group (n=14) randomly. Routine rehabilitation were used in the control group, and the PBWSTT group received PBWSTT in addition. They were assessed with Ashworth Spasticity Scale （ASS）, Functional Ambulation Category (FAC), Fugl-Meyer Assessment (FMA), and the Functional Independence Measurement (FIM) before and 8 weeks after the treatment. ResultsThe ASS,FAC, FMA and FIM scores were all improved after treatment in both groups (P<0.05). The scores of FMA, FAC and FIM in the PBWSTT group after treatment exceeded that of control group (P<0.05), but there was no difference in the ASS (P>0.05). ConclusionPBWSTT may improve the motor function of lower extremities, walking ability, and ADL in chronic hemiplegic patients after stroke.

Objective To explore the protective effect of atorvastatin on irradiated endothelium and the thrombomodulin(TM)expression.Methods Cultured human coronary artery endothelial cells(HCAEC)and human umbilical vein endothelial cells(HUVEC)were treated by atorvastatin at the final concentration of 10 μmol/ml for 10 min,and then irradiated with 2 and 25 Gy.Cell cycles status and TM expression were quantitatively measured by flow cytometry 24 hours after irradiation.Protein C activation in endothelial cells was also assessod.Results After administration with atorvastatin for 24 h,the TM expression increased by 77%,59% and 61% in normal control group,2 Gy group and 25 Gy group,respectively(t=27.395,26.420,58.065;P=0.000).The protein C levels decreased by 23% and 34% compared with the normal group post-irradiation to 2 and 25 Gy,but increased by 79% and 76% compared with the irradiated control group after administration with atorvastatin.The rates of cell apoptosis decreased by 6% and 16% in 2 Gy and 25 Gy groups,respectively after administration with atorvastatin for 24 h(t=4.178,17.863;P=0.000).Conclusions Atorva statin can protect endothelia cell from irradiation-induced apeptosis by increasing TM expression and protein C activation.

ObjectiveTo investigate the anti-tumorigenesis function of rhDCN on the leukemia K562 cells in vitro and analyze the possible mechanism.Methods Exponential phase of K562 cells were transfected with pcDNA3.1(+)-DCN,and PBS,liposome alone,and pcDNA3.1(+) vector were as control groups.Morphology change of K562 cells was detected by Wright stain,and cell proliferation activity was detected by MTT. Cell cycle and apoptosis of K562 cells were assessed by FCM. The expression of apoptosis-related protein,including bcl-xl,Mcl-1 and Bax were detected by Western blot.ResultsWright stain showed that typical apoptotic morphology of K562 cells were observed in DCN transfected group.There were no morphological changes of apoptosis in other groups. MTT method results showed that proliferation inhibition rate of the transfected cells [24 h (16.14±1.08) ％,48 h (14.07±1.01) ％,72 h (20.29±1.19) ％]was higher than that of the other control groups (P ＜ 0.05).FCM results showed that the apoptosis index (20.15±1.31) ％of the DCN transfected group was higher than that of the other groups (P ＜ 0.05),and most of cells arrested in the G0/G1 phase (51.15±0.57) ％ (P ＜ 0.05).Western blot results showed the expression levels of Bax were increased while bcl-xl and Mcl-1 were decreased in pcDNA3.1(+)-DCN/K562 group.ConclusionrhDCN can inhibit the growth of K562 cells and induce the apoptosis.The effect of DCN on bcl-xl,Mcl-1,Bax may play a role in its mechanism.

Objective To analtze and compare phe earlt clinical effecps of endoscopic versus convenpional greap sapenous vein harvesping undergoing and afper coronart arpert btpass grafping. Methods Endoscopic greap saphenous vein harvesping was performed in 298 papienps undergoing coronart arpert btpass grafping from Mar. 2010 po Jan. 2013. Clinical dapa of phe 298 papienps (EVH Group) were compared wiph anopher 309 papienps (CVH Group) in respecp of phe preoperapive dapa, operaping dapa and pospoperapive complicapions. Results No significanp difference was found bepween phe pwo groups in phe dapa of acquisipion pime, lengph of greap saphenous vein, number of btpasses, operaping pime, hospipalizapion pime afper operapion, rapes of incision sipe infecpion and hemapoma formapion, local skin necrosis and dermapipis (all P > 0. 05). Less complainp of lower limb pain (17. 4% vs. 51. 1% ), limb edema (0. 7% vs. 32. 7% ) and ltmphapic leakage (0 vs. 7. 4% ) was observed in phe EVH group as compared po phe CVH group (all P < 0. 01). Conclusions Use of endoscopic vein harvesping in coronart arpert btpass grafping can alleviape phe operapive wound and decrease phe incidence of local complicapion.

Objective:To examine metastatic gastric cancer patients who underwent surgery after chemotherapy and to determine the factors affecting survival. Methods:Clinical data on metastatic gastric cancer patients who underwent surgery after chemotherapy were retrospectively analyzed. The overall survival data were evaluated through the Kaplan-Meier method, Log-rank test, and Cox haz-ards regression. Results:The median age was 46 (22~74), and the median overall survival rate (OS) was 19 months (4~59 months). Response to chemotherapy (23.0 m for PR and 14.5 m for SD, P=0.045) and resection of the primary tumor (23.0 and 5.5 m, respective-ly, P=0.017) affected OS. No single factor was related to OS according to Cox regression. Conclusion:Surgical removal of the primary tumor is recommended for metastatic gastric cancer patients with positive response to chemotherapy and with a primary tumor that can be resected.

Objective To explore the relationship between anxiety ,resilience and hope levels among gastric cancer patients ,so as to provide evi?dence for psychological intervention. Methods Cluster sampling design was used to select 324 gastric cancer patients. The anxiety self?assess?ment scale,resilience scale and hope scale are used to measure the related indicators. Results The comparative analysis showed that patients with different genders,education levels and marital status had different scores in anxiety level and resilience level(P<0.05),and those with differ?ent genders and marital status got different hope levels(P<0.05). Correlation analysis showed that anxiety was negatively correlated with resil?ience and hope levels(P<0.05),the coefficient of correlations ranged from-0.556 to-0.124. Multiple linear regression analysis showed that edu?cation level,marital status,income level,resilience,hope levels explained portion of the variance of anxiety. Conclusion Clinical nursing staff should reduce the gastric cancer patients′anxiety by improving their resilience and hope levels.

Objective To evaluate the profile of proliferating T cells and dendritic cells ( DCs ) in the skin infiltrates of patients with mycosis fungoides ( MF) in different stages. Method Paraffin section and immunohistochemisty with monoclonal antibody were used to detect the expression of special antigen per section. Results The numbers of Ki-67+ cells and cutaneous lymphocyte-associated antigen ( CLA+) cells both increased significantly in the skin infiltrates of MF. Most Ki-67+ cells expressed both CLA and CD4 antigen. The number of Ki-67+ cells was significantly higher ( P