Objective To investigate the expression of phosphatase and tensin homolog gene (PTEN) and 1-phosphatidylinositol 3-kinase (PI3K) in rectal cancer and its correlation with clinical pathology.Methods MaxVisionTM immunohistochemical method was used to detect the expressions of PTEN and PI3K in 60 rectal cancer tissues,30 adenoma tissues and 10 normal rectal tissues,and the correlation between the expression levels and its clinicopathologic characteristics was discussed.Results The positive expression rate of PTEN in rectal cancer tissue was significantly lower than that in normal rectal tissue and adenoma tissue [43.3％ (26/60) vs.100.0％ (10/10),93.3％ (28/30),P ＜ 0.05].The positive expression rate of PI3K in rectal cancer tissue was significantly higher than that in normal rectal tissue and adenoma tissue [75.0％ (45/60) vs.30.0％ (3/10),33.3％ (10/30),P ＜ 0.05].The positive expression of PTEN and PI3K were correlated with carcinoembryonic antigen,degree of cell differentiation,TNM stage,lymph node metastasis and distant metastasis(P＜ 0.01 or ＜ 0.05 ),but had no correlation with age,gender,neoplasm location,neoplasm appearance (P ＞ 0.05).The correlated analysis showed that the positive expression of PTEN had negative correlation with PI3K (r =-0.269,P =0.023 ).Conclusions The high expression of PI3K and down regulation of PTEN in rectal cancer are closely related to its invasion and metastasis.There are some values to monitor PTEN and PI3K expression for determining biological behavior of rectal cancer.

Post-polio syndrome involves a variety of clinical manifestations, which need multi-dimensional evaluation measurement. Multi-dimensional Fatigue Inventory (MFI-20), muscle strength testing, laboratory test, imaging study, the sleep quality assessment, electro-physiological test, pain score, functional independence measure, moving obstacles evaluation, physical activity situation, walking ability as-sessment, the Medical Outcomes Study health survey short form, and evaluation of mental health scale are in common use in the studies.

Post-polio syndrome (PPS) usually appears decades after acute polio infection, characterized as progressive muscle weak-ness, fatigue, pain, muscle atrophy, poor endurance, intolerance of cold, sleep apnea, water choking cough, and difficulty in swallowing, etc., resulting in a decline in physical function. As an insidious disease, it is very important to identify and diagnose PPS.

Post-polio syndrome (PPS) is a syndrome after acute infection of poliovirus and sequelae of polio. The incidence of PPS is high, and more in old patients. The pathological features of PPS include chronic nerve damage affected muscles, result in fatigue, pain, breathing and sleep disorders, fall risk, and so on, which impair their health and quality of life. The hypotheses of pathogenesis of PPS in-clude over load of motor neurons, and the continuous existence of the poliovirus, etc. PPS is a stable neuromuscular disease progressing slowly.

There is no specific treatment for post-polio syndrome. The common applied therapies include mediciation, exercise, cogni-tive behavioural therapy, physiotherapy, occupational therapy, Traditional Chinese Medicine, assistive technology, psychological and social factors adjustment, interdisciplinary comprehensive rehabilitation, and so on.

Objective To investigate the expression of metastasis associated gene-1 (mag-1) and mammalian target of rapamycin (mTOR) in rectal cancer, and its correlation with clinical pathology. Methods The expressions of mag-1 and mTOR in 60 rectal cancer tissue, 30 adenoma tissues and 10 normal rectal tissues were detected by immunohistochemical method, and the correlation between the expression levels and rectal cancer clinical pathologic characteristics was discussed. Results The positive expression rates of mag-1 and mTOR in rectal cancer tissue were significantly higher than those in normal rectal tissue and adenoma tissue:55%(33/60) vs. 1/10 and 27%(8/30), 58%(35/60) vs. 2/10 and 30% (9/30), and there were statistical differences (P<0.05). The expression levels of mag-1 and mTOR in rectal cancer tissue were correlated with carcinoembryonic antigen on admission, degree of cell differentiation, TNM stage, lymph node metastasis and distant metastasis (P<0.01 or<0.05), but had no correlation with age, gender, neoplasm location and neoplasm appearance (P > 0.05). The correlation analysis result showed that the expressions of mag-1 and mTOR were positively correlated in rectal cancer tissue (r=0.730, P<0.01). Conclusions The mag-1 and mTOR may correlate with invasion and metastasis in rectal cancer, and monitoring mag-1 and mTOR expression has a certain value for determining biological behavior of rectal cancer.

Aim To determine the plasma protein binding rate of five components of Eucommia ulmoides extract. Methods The equilibrium dialysis method was used to study the plasma protein binding rate. The plasma samples were extracted by protein precipitation with methanol. With the use of puerarin as the internal standard, UPLC-MS/MS was carried out to determine the concentration of the five compounds in and out of the dialysis membrane. Results The average plasma protein binding rates of five compounds on the area of the concentration which was determinate were as fol-lows, respectively: geniposidic acid was ( 25. 77 ± 2. 68 )%, protocatechuic acid was ( 57. 54 ± 3. 79)%, chlorogenic acid was (53. 91 ± 3. 00)%, pinoresinol diglucoside was (24. 15 ± 4. 92)%, and pinoresinol singleglucoside was (49. 78 ± 3. 61)%. Conclusions The results show that the binding percentage of geniposidic acid and pinoresinol diglucoside is relatively low, but the binding rate of the others with rat plasma protein is moderate.

Objective To set up a three-dimensional finite element model (FEM) to investigate biomechanics of point contact locking plate (PC-LP) fixating femoral shaft fractures. Methods One intact fresh adult cadaveric femur was scanned by CT at 1 mm interval. Then, the data of CT were utilized to establish three-dimensional FEM by using software Mimics and PRO/E and simulate the different clini-cal loading conditions. The changes of theoretical stress of femur and PC-LP were analyzed under flexion, axial compression and torsion loads. Results (1) Under four-point bending load, the distribution of femur stress was in uniformity, with the largest stress of the PC-LP focused on the edge. (2) Under axial compression load of 250 N, the largest stress of the femur was focused on the screw holes on beth distal ends, with the largest stress of the PC-LP focused on the middle screw holes. (3) Under the torsion load cused on the middlepart and the middle screw holes. Conclusions Under the four-point bending, ax-ial compression and torsion loads, the distribution of femur stress is in uniformity, when the largest stress of the PC-LP focuses on edge or the middle screw holes, while that of the PC-LP on two screw holes of proximal or distal ends.

Objective:To explore the relationship of optimal pulse-taking pressure among cun,guan,chi pulse of of healthy persons.Methods:264 Healthy undergraduate were included and pulse signals were collected from cun,guan,chi in cunkou area.The relationship of optimal pulse-taking pressure among three-region pulse,ratios and body mass index(BMI)were researched.At the same time,to calculate relationship of optimal pulse-taking pressure among cun,guan,chi pulse.Results:The optimal pulse-taking pressure of cun,guan,chi pulse was positively correlated with BMI.The optimal pulse-taking pressure of guan and cun pulse was significantly smaller than that of chi pulse.The optimal pulse-taking pressure of cun pulse is similar to that of guan pulse,which was 90 percents of the chi pulse.Conclusion:There was significant difference in optimal pulse-taking pressure between cun,guan,chi pulse.The result was favourable to the optimizing the program of pulse device,consummating of three-probe pulse sensor and studying pulse thoroughly.

Objective To understand the gender selection and prognosis of children with 46,XY disorders of sex development (DSD) after surgery,and to provide reference for future clinical decision-making.Methods Data of 85 (80 males and 5 females) postoperative patients with 46,XY DSD with follow-up age of 6(4,11) years who were treated at the Department of Endocrinology,Genetics and Metabolism of Beijing Children's Hospital Affiliated to Capital Medical University during the period from September 2009 to April 2018 were retrospectively analyzed.The patients were grouped based on diagnosis.The basis of postoperative gender selection,patient satisfaction and related factors,gender characteristics,and adolescent development were analyzed.The Pre-school Activities Inventory or the Children's Sex Role Inventory were used in the analysis of gender tendency.Mann-Whitney U test was used to compare postoperative gender satisfaction of different factors.The Kruskal-Wallis method was used to compare the postoperative gender satisfaction of each group.Fisher's test was used to compare the follow-up status of male children over 11 years old in each group.Results Among the 85 patients,62 individuals were raised as girls after birth,9 were facultative and 14 as boys.According to the diagnosis,there were 31 individuals in group 1 (with 5α-reductase deficiency),11 individuals in group 2 (with androgen insensitivity syndrome),9 individuals in group 3 (with NR5A1 gene mutation),4 individuals in group 4 (with hypergonadotropic gonadal dysplasia),and 30 indiviudals in group 5 (with unclear diagnosis and normal human choionic gonadotophin test).Among the 71 children who were raised as girls or facultative children after birth,66 selected as boys,and 5 continued as girls (among them,3 individuals were female with passive selection,and 2 individuals of testicular dysplasia with uterus in group 4 and 5 were female with active selection).Among the 71 patients faced with gender selection,only one was unsatisfied,that was a postoperative female.There was no significant difference in postoperative gender satisfaction among different disease diagnoses,surgical age and penis length (x2(H)=6.007,P=0.199;Z=-0.860,P=0.390;Z=-0.438,P=0.661).Fifty-nine of the 85 cases completed the gender tendency scale test and 46 cases (78％) were consistent.In the male patients,45 cases were consistent.Thirteen inconsistent patients (22％) were female or facultative after birth who were 5 years old or older.There was no stigmatization noticed in the inconsistent patients' daily life and school social settings.There were 22 male patients aged 11 years and older.They were 13(12,16) years old.Fourteen (64％) individuals' penile length reached the normal minimum,15 (68％) individuals' testicular volume were equal or more than 4 ml,16 (73％) individuals' sex hormones entered puberty levels,12 (55％) individuals had been spermatorrhea,the age of first spermatorrhea was (13.3±2.4) years.They were satisfied and adaptable after surgery.There was no significant difference in the above indicators among the groups (x2 =2.999,P=0.694;x2 =7.278,P=0.086;x2 =5.597,P=0.358;x2 =6.904,P=0.127).Conclusions The appropriate gender of 46,XY DSD patients was selected according to gonadal status after diagnosis.Regardless the diagnosis,the age of operation and the length of the penis at the first diagnosis,male patients were satisfied with the gender after the operation.A few of patients were inconsistent with the results of gender tendency scale test who were raised as girls or facultative children after birth,and they required sustained special attention.Some of the children showed natural adolescent development in males,and the prognosis may be ideal.