Objective To examine the effect of somatostatin in the treatment of adhesive intestinal obstruction by meta-analysis. Methods Researchers extensively searched CNKI, Wanfang, and Pubmed and included articles which met the inclusion criteria.For each included article, researchers extracted the research methodology and the related indicators including gastrointestinal decompression, abdominal distension time, exhaust time and hospitalization time.We selected fixed effect models or random effect models to combine the effect of each variable according to the heterogeneity test results.Results Somatostatin could significantly reduce the amount of gastrointestinal decompression -213.19 mL/d (-252.97,-173.40), shorten the abdominal distension time-1.42 d( -1.71,-1.14), exhaust time-1.39 d( -1.79,-0.98), and the hospitalization time-3.46 d( -4.10,-2.83).Conclusion Somatostatin is effective in the treatment of adhesive intestinal obstruction.

Objective To evaluate the efficacy of laparoscopic operations in the treatment of ectopic pregnancy.Methods A total of 148 cases of tubal pregnancy from January 2002 to January 2005 were studied.Laparoscopic operations included 106 cases of oviduct-sparing operation(79 cases of oviduct fenestration and 27 cases of lesion clearance),37 cases of salpingectomy,and 5 cases of lesion clearance.Results The diagnosis and operation were laparoscopically completed in all the 148 cases.Persistent ectopic pregnancy occurred in 2 cases.Postoperative monitoring of serum ?-hCG levels showed the shortest recovery time to normal levels after the treatment of salpingectomy(8?1 d),and the longest one after lesion clearance (15?2 d).Regular menstruation resumed at((31?)2 d) after salpingectomy and at(43?3 d) after lesion clearance.Follow-up checkups with uterotubography in 84 cases for 1~3 months(mean,2.3 months) found a patent rate of both oviducts of 82.1%(69/84).Conclusions Laparoscopic operations is a safe and effective option in the treatment of ectopic pregnancy.

Objective To investigate the relationships between serum concentration of RBP-4 and inflammatory factors,insulin resistance in patients with gestational diabetes mellitus.Methods20 patients newly diagnosed with impaired glucose tolerance (IGT),27 patients newly diagnosed with gestational diabetes mellitus(GDM) and 50 subjects with normal glucose tolerance(NGT) were enrolled in this cross-sectional study.Additionally,another subset analysis was performed,subjects were divided on the basis of BMI into normal weight (NW) and overweight or obesity (OW/OB).Serum RBP-4 was measured with ELISA;serum concentrations of hs-CRP,free fatty acids (FFA),triglyeride(TG),total cholesterol (TC) and insulin were measured in fasting status.Insulin resistance was assessed by HOMA-IR.ResultsLn(HOMA-IR) and the concentrations of FFA,ln(hs-CRP) in IGT or in GDM were significantly higher than that of NGT(P＜0.05).In the group of OW/OB,FFA[(0.90±0.31)mmol/L vs (0.71±0.28)mmol/L,t＝2.73,P＜0.05)],ln(hs-CRP)(0.62±1.00 vs -0.17±1.07,t＝2.90,P＜0.05),ln(HOMA-IR),lnRBP(0.56±0.27 vs 0.30±0.14,t＝2.86,P＜0.05)were significantly higher than that of NW.In the group of GDM,lnRBP-4 showed a positive relationship with FFA (r＝0.231,P＜0.05)and ln(hs-CRP) (r＝0.237,P＜0.05).There was a positive relationship between lnRBP-4 and FFA(r＝0.371,P＜0.05) or ln(hs-CRP)(r＝0.247,P＜0.05) in the group of OW/OB,but no association in the group of NGT,IGT or NW.There was no relationship between lnRBP-4 and ln(HOMA-IR) in IGT,GDM and NW,while in the group of NGT and OW/OB,lnRBP-4 showed a positive relationship with ln(HOMA-IR) (r＝0.276,0.290,P＜0.05).In a multiple linear regression analysis,ln(hs-CRP),FFA or ln(HOMA-IR) was not the independent factor of lnRBP-4 in any group.ConclusionsIn patients with gestational diabetes mellitus,lnRBP-4 may be a new marker of inflammation.

Objective · To investigate the role of interleukin-10 (IL-10) in regulating ocular neovascularization (NV). Methods · Expression of IL-10 was investigated in mice with oxygen-induced retinopathy (OIR) and transgenic mice with VEGF expression in photoreceptors by immunofluorescence,RT-PCR, and Western blotting. Mice deficient in IL-10 were used to test the effect of IL-10 in retinal, sub-retinal, and choroidal NV. Results · In OIR mice and transgenic mice with VEGF expression in photoreceptors, the staining intensity and mRNA expression of IL-10 were increased. Mice deficient in IL-10 showed a significant reduction in ischemia-induced retinal NV, and choroidal NV at rupture sites in Bruch's membrane. Mice lacking IL-10 showed reduced levels of hypoxia-inducible factor-1α (HIF-1α) and suppression of ischemia-induced expression of VEGF and VEGF receptor 1. Macrophage was regulated and reduced in ischemic retina of mice with IL-10 deficiency. Conclusion · IL-10 stimulates ocular NV through modulation of HIF-1α and its target genes VEGF and VEGF receptor 1. IL-10 promotes ocular NV via macrophage response to retina ischemia.

BACKGROUND:Bone marrow mesenchymal stem cells (BMSCs) transplantation can promote cardiac repair after myocardial infarction, but it has been limited by the low cellsurvival rate.
OBJECTIVE:To study the effect of hydrogen sulfide (H 2 S) on the BMSCs transplantation for treatment of myocardial infarction.
METHODS:BMSCs were separated and cultivated form Sprague-Dawley rats weighing (100±20) g. The 4th generation cells were used for later experiment, and marked by DAPI at 2 hours before use. Fifty male Sprague-Dawley rats, weighing (200±20) g had been divided into five groups:Sham group (n=10) and four transplantation groups:BMSCs (n=10), H 2 S-BMSCs (n=10), H 2 S (n=10), normal saline (n=10). The myocardial infarction model of four groups was established except of sham group (only thread without ligation). The cardiac function was measured by echocardiogram at 4 weeks after celltransplantation. The col agen in the infarction area was tested by Masson staining.
RESULTS AND CONCLUSION:Severe myocardial fibrosis was found in the normal saline group, with no myocardial regeneration in the infarct area. H 2 S-BMSCs group had less col agen and more cardiac muscle tissue than BMSCs or H 2 S groups. Left ventricular ejection fraction and left ventricular fractional shortening of the H 2 S-BMSCs group were significantly higher than those of the BMSCs or H 2 S groups (P<0.05). The cells survival rate and cardiac function of myocardial infarction rats can be promoted by H 2 S-preconditioned BMSCs transplantation, which is superior to BMSCs or H 2 S alone.

BACKGROUND:Ischemia microenvironment contributes mostly to the low survival rate of rat bone marrow mesenchymal stem cells after transplantation. Hydrogen sulfide (H2S) can protect various cells and tissue models against apoptosis and injury.
OBJECTIVE:To detect the cellapoptosis and viability, content of H2S in supernatant, and the expression of H2S synthetase after different time of hypoxia and serum deprivation cultivation of rat bone marrow mesenchymal stem cells.
METHODS:The passage 3 rat bone marrow mesenchymal stem cells were divided into five different cultivation time groups:0-, 3-, 6-, 12-and 24-hour groups. After enough hypoxia and serum deprivation cultivated time, the cellapoptosis was detected by SubG1, the cellviability was determined by cellcounting kit-8, the content of H 2S in supernatant was measured by N,N-dimethyl-p-phenylenediamin and the expression of H2S synthetase by RT-PCR and western blot.
RESULTS AND CONCLUSION:Compared to the normal cultivation group, after different hypoxia and serum deprivation cultivated time, the cellapoptosis increased and cellviability decreased significantly. The longer hypoxia and serum deprivation cultivated time caused the more cellapoptosis and the lower cellviability. The contents of H2S and its synthetase were also suppressed by hypoxia and serum deprivation cultivation. The difference was statistical y significant. These findings suggest that hypoxia and serum deprivation cultivation can inhibit the generation of H 2 expression of its synthetase.

Objective: To investigate risk perception, emergency preparedness and emergency literacy among medical students under COVID-19 epidemic. Methods: An online survey was conducted by using the self designed COVID-19 questionnaire on risk perception, emergency preparedness and emergency literacy from March 27 to March 30, 2020. The convenience sampling method was used to investigate 4 864 medical students from 2 colleges and technical secondary schools in Sichuan Province. Results: The scores of risk perception, emergency preparedness and emergency literacy associated with COVID-19 were(35.18±4.33)(28.30±4.16) and(25.23±2.97) respectively. Risk perception of medical students was positively correlated with emergency preparedness and emergency literacy(r=0.35, 0.40), emergency preparedness was positively correlated with emergency literacy(r=0.51)(P<0.05). Conclusion Considering interrelationships among risk perception, emergency preparedness and emergency literacy associated with COVID-19, medical students should be given targeted prevention and control training.

Objective To build up one bonus shares method of improvi ng nursing quality in order to simulate nurse′s the inner enthusiasm.Methods The competence model and assessment system were established for one important weight for bonus sharing of competence qualified of nursing staff,competence coefficient and performance assessment.Results The implementation of performance assessment improved nurse′s satisfaction from 76.3% to 89.2%,while the parent′s satisfaction increased from 84.3% to 93.0%(χ2 =1 3.070,9.766;P <0.05).Conclusions The bonus shares method is scientific and reasonable,which can dig out the potential of nursing staff and is worthy of promotion.

Objective To understand the current situation of time management disposition in pediatric nurses and to analyze its influencing factors so as to put forward targeted countermeasures.Methods A total of 571 nurses from 3 Class III Children′s Hospitals of Jiangsu Province were selected as research objects by convenient sampling. They were investigated with the general condition questionnaire and the time management disposition inventory.Results The total score of time management disposition was (59.20±8.17) points with 84.06% of the pediatric nurses in the middle level. There were statistically significant differences in time management disposition among different ages, positions, years of service ( F=2.838,-3.080, 2.978;P<0.05) . Stepwise regression analysis showed that the positions were the major factor to forecast the time management disposition of nurses in Class III Children′s Hospitals.Conclusions The time management disposition of nurses in Class III Children′s Hospitals is in the middle level and its major influencing factor is the positions.

Objective:To analyze the application of sensory stimulation based on the whole process accompanying of relatives in regain of consciousness in coma patients after aneurysm embolization.Methods:Comatose patients after aneurysm embolization in Department of Cerebrovascular Disease, Suining Central Hospital of Sichuan Province were selected as the research objects. 59 patients from June to December in 2019 were included in the control group and received routine sensory stimulation. 60 patients from January to August 2020 were included in the study group, and the sensory stimulation based on the whole process accompanying of relatives was implemented. Glasgow Coma Scale (GCS) , Chinese version of Coma Recovery Scale-Revised (CRS-R) and Mini-Mental State Examination (MMSE) were applied to compare the coma status, recovery status and cognitive function of the two groups before and after the intervention. The complications of the two groups were compared as well.Results:After 3 months of intervention, the scores of GCS, CRS-R and MMSE of the study group were all higher than those of the control group, with statistically significant differences ( P<0.05) . Within 3 months after operation, the total incidence of complications in the study group was lower than that in the control group, and the difference was statistically significant ( P<0.05) . Conclusions:Sensory stimulation based on the whole process accompanying of relatives is helpful to reduce the degree of coma, promote the recovery of patients, and reduce the incidence of complications.