Via activating G-protein receptor transduction pathways,nitric oxide (NO),an important signal molecule,has a complex and diverse role which is closely related with activity and gene expression of nitric oxide synthetase (NOS).NO is involved in tumor related events such as cellular proliferation,migration,especially the angiogenic process,and it is closely related with the occurrence and progression of prostate cancer.NO donors and NOS inhibitors have anti-cancer and radio-chemotherapy enhancement roles.

Long non-coding RNA was initially identified as "noises" of gene transcriptions. However, with the developing researches of ENCODE, it was found that the long non-coding RNAs can regulate the genomic expressions in the form of RNAs in epigenetic, transcription, and post transcriptional levels, which is involved in the regulation of diverse cellular processes and has significant influences on occurrence and precaution of human diseases. This paper introduces functions and features of the long non-coding RNAs, and sums up the internal relation between long non-coding RNAs, diabetes and diabetic complications on the basis of existing researches. These advances can provide the basis for the further understanding of molecular medicine on occurrence and evolution of diabetes.
Diabetes Complications ; genetics ; Diabetes Mellitus ; genetics ; Humans ; RNA, Long Noncoding ; genetics

Objective To verify the mediating effect of the moral identity on the relationship between sport moral disengagement and pro-social and anti-social behaviors in competition.Methods Two hundreds and sixty-seven athletes of group events(including 173 males,94 females,with an average age of 22.40,SD=2.75)were selected and investigated using the sport moral disengagement scale,moral identity scale and the scale of pro-social and anti-social behavior in competition.Results (1) The sport moral disengagement had significant influence on the athletes'pro-social and anti-social behaviors in competition.(2)The moral identity has significant positive influence on the athletes' pro-social behavior in competition,but significant negative influence on the athletes' anti-social behavior in competition.(3)The moral identity has significant mediating effect on the relationship between sport moral disengagement and pro-social behavior in competition.Conclusion The moral disengagement in sport has important influence on the athletes' pro-social and anti-social behavior in competition.The moral identity has a significant mediating effect on the relationship between sport moral disengagement and pro-social behaviors in competition.

OBJECTIVE To test and analyze the new drug-resistant characteristics of Acinetobacter baumannii.METHODS The bacteria identification was determined by the routine method.Antimicrobial susceptibility was detected by K-B test.RESULTS In 95 A.baumannii strains,in addition to imipenem,the drug-resistant incidence was above 49%.There were two A.baumannii strains resistant to imipenem,and were seen panresistant strains The new characteristics of drug-resistance were found.CONCLUSIONS A new drug-resistant mode about A.baumannii to amikacin is found.The high drug-resistant incidence of A.baumannii becomes a very important problem.

Objective:To explore the effect of the labor abdominal pressure training device on the second stage of labor and the outcome of labor in primiparas undergoing epidural analgesia.Methods:The healthy primiparas who had patient controlled epidural analgesia were randomly divided into the observation group (126 cases) and the control group (122 cases). The control group used conventional oral instruction to force after entering the second stage of labor. The women in the observation group used the labor abdominal pressure training device to guide the force. The time required to learn to exert force, the second stage of labor and the outcome of labor were observed.Results:The parturient women learned the correct time to exert force, the time of the second stage of labor, the self-efficacy score of the second stage of labor, and the post-natal fatigue score were (13.56 ± 5.32) min, (76.32 ± 10.58) min, (121.38 ± 17.52)points, (8.56 ± 0.72) points in the observation group, and (25.47 ± 7.65) min, (105.21 ± 18.41) min, (101.42 ± 14.32) points, (13.31 ± 0.46) points in the control group, the differences between the two groups were statistically significant ( t values were -10.326-11.002, all P<0.05). The maternal spontaneous delivery rate, fetal assisted delivery rate, forceps assisted delivery rate, perineal lateral cut rate, perineal median cut rate, perineal tear>Ⅰ°rate were 94.44% (119/126), 4.76% (6/126), 0.79% (1/126), 9.52% (12/126), 11.11% (14/126), 6.35% (8/126) in the observation group, and 85.25% (104/122), 12.30% (15/122), 2.46% (3/122), 29.51% (36/122), 4.10% (5/122), 9.84% (12/122) in the control group,the differences between the two groups were statistically significant ( χ2 values were 4.582-9.437, P<0.05 or 0.01). Conclusions:The use of an abdominal pressure training device for labor in patient controlled epidural analgesia for pregnant women can shorten the second stage of labor and study the time of exertion, improve the self-efficacy of the second stage of labor, reduce the degree of perineal injury and fatigue, reduce the vaginal birth rate, and help protect Mother and child are safe.

BACKGROUND: Severe acute respiratory syndrome (SARS) is a kind of very strong infective respiratory system diseases. Due to rapid development of disease course, severe symptoms, forced isolation, being away the supports from relatives and friends, many patients with SARS presented extremely anxiety and panic, even depression and despair, and they are in the crisis state and need mental support and help.OBJECTIVE: To evaluate the therapeutic effect of mental crisis intervention in patients with SARS.DESIGN: Controlled observation.SETTING: Xiaotangshan Hospital of Chinese PLA.PARTICIPANTS: Totally 680 patients with SARS who received the mental intervention in the Psychological Counseling Center, Xiaotangshan Hospital of Chinese PLA in May 2003. The patients, 379 male (55.74%)and 301 female (44.26%), were aged (35±7) years. Degree of education:34.5% of patients received education below junior high school, 31.5% senior high school and 34.0% college. 100% of the patients had family member or colleague-infected life events, and 14.4% of the patients lost their family members. Degree of disease: 6.9% of patients were in the severe period, 67.5% in the stable period and 25.6% in the rehabilitation period.METHODS: ① Totally 489 patients who received telephone counseling,and attended telephone mutual-help group, group debugging, individual counseling and group counseling were chosen, serving as intervention group, and the other 191 patients served as control group. ②The mental health state of patients in the 2 groups was evaluated at admission with symptom checklist 90 (SCL-90), which consisted of somatization, obsessive compulsive, interpersonal sensitivity, depression, anxiety, hostility, phobic anxiety, paranoid ideation and psychoticism 9 factors. The grading scoring was used. The higher grades, the more severe symptoms. ③ The patients of intervention group were given comprehensive mental intervention, which included handing out materials(self-debugging propagandist manual), care from staff, mental counseling by telephone, group debugging, individual counseling, guidance by mental doctor, etc. Patients in the control group only received handed materials (self-debugging propagandist manual) and the care from staff. SCL-90 was used to re-evaluate the patients of intervention group and control group before discharge. ④Intervention therapeutic effect of patients in 2 groups were evaluated with comprehensive mental intervention questionnaires, which were mainly about self and social cognition, feeling and emotion, life behavior styles, physiological and body symptoms 4 dimensions. Grading scoring was used. The higher points, the better intervention effect. Self-rating and rating by others were used in each question. If the results in self-rating are the same as those in rating by others, questionnaire is valid, otherwise, it is invalid). ⑤ The scoring of all the patients at admission was compared with that of Beijing norm by using SCL-90.The mental health of patients of 2 groups after intervention was measured, evaluated and compared with SCL-90.MAIN OUTCOME MEASURES: Difference in scoring of SCL-90 between all the patients and Beijing norm; Changes in the scoring of SCL-90 between intervention group and control group at admission and after intervention.RESULTS: Totally 680 comprehensive mental intervention questionnaires were handed out, and 669 of them were retrieved, among which 483 from intervention group and 186 from control group. Altogether 669 patients participated in the result analysis. ① Points of each factor in SCL-90 of patients at admission were significantly higher than norm, especially in anxiety and phobic anxiety. 45.5% and 50.1% of the investigated patients had more than 3 points in anxiety and phobic anxiety respectively. 86.5% of the investigated patients had mental problems to different degrees. ②There was no significant difference in the points of various factors in SCL-90 at admission between intervention group and control group (P＞0.05).In the various factors of SCL-90, no significant difference in the points of various factors in SCL-90 at admission between intervention group and control group (P＞0.05).In the various factors of SCL-90,no significant difference exists in phobic anxiety and paranoid ideation and psychoticism between two groups (P＞0.05),but the points of the other factors of intervention group were significantly lower than those of control group (P＜0.05-0.01).CONCLUSION: Mental crisis intervention can remarkably lighten the somatization, obsessive-compulsive, anxiety and depression, lower interpersonal sensitivity and improve hostility.

Objective: To explore the effects of rhPRL on the cytotoxicity and proliferation of natural killer cell lines. Methods: Cytotoxici-ty and proliferation analysis were done by MTT,RT-PCR method,direct immunofluoresence technique and FAGS protocol was applied to detect the PRL-R and CD25 expression on NK cell lines. Results: MTT results indicated that PRL play a significant roles on the proliferation and cytotoxicity of IL-2 dependent cell lines NKL and NK-92 in the presence of trace amounts of IL-2. In contrast, PRL did not significantly alter the proliferation and cytolyric activity of YT. RT-PCR and FACS analysis showed three NK cell lines expressed PRL-R and CD25 differently, stimulation of rhPRL could upregulate CD25 and IL-2 expression. Conclusion: rhPRL could augument the proliferation and cytotoxicity of NK cell partly by upreglating of IL-2. Ra chain(CD25) and interaction with IL-2 signal transduction.

OBJECTIVE To investigate the ?-lactamases gene of multi-drug resistant Acinetobactor baumannii(MDR-ABA).METHODS The blaTEM,blaSHV,blaPER,blaGES,blaIMP,blaVIM,blaSIM,blaOXA-23,blaADC and blaDHA genes of 20 MDR-ABA strains were detected by PCR.RESULTS Among 20 MDR-ABA strains,18 strains were with positive blaADC,11 strains were with positive blaTEM;the others were negative.Totally 19 strains were with genes correlated to ?-lactam antibiotics.The results indicated that the blaADC DNA sequence was a new subtype type compared with the blaADC sequence which had registered on America GenBank.CONCLUSIONS blaADC Gene and blaTEM gene have spread in the MDR-ABA group and a new blaADC subtype has been found.

OBJECTIVE To investigae the gene resistant to quaternary ammonium compounds of multiple drug resistant Acinetobacter baumannii(MDR-ABA).METHODS The quaternary ammonium compounds qacE?1 gene in 20 MDR-ABA strains were detected by PCR.RESULTS The 20 MDR-ABA strains showed multiple resistance,its sensitivity to imipenm was 65% only.Among twenty strains of MDR-ABA qacE?1 gene were all positive.CONCLUSIONS The results indicate that qacE?1 gene were all carred class 1 integron.The chlorhexidine usage in prevnting hospital infection after surgery should be reevaluated.

Understanding and learning the up-date clinical information of evidence-based medicine are the important part of knowledge for clinical postgraduates and are the basic ability required in their future clinical work.The necessity and role of literature retrieval course were studied with meta analysis of literature retrieval course teaching as the core content,and with meta analysis of literature retrieval course teaching as the writing objective.The literature retrieval course teaching process was displayed by demonstrating the case design and analysis,retrieval methods and their implementation in different stages of literature retrieval course teaching.