Objective To investigate the relationships between serum concentration of RBP-4 and inflammatory factors,insulin resistance in patients with gestational diabetes mellitus.Methods20 patients newly diagnosed with impaired glucose tolerance (IGT),27 patients newly diagnosed with gestational diabetes mellitus(GDM) and 50 subjects with normal glucose tolerance(NGT) were enrolled in this cross-sectional study.Additionally,another subset analysis was performed,subjects were divided on the basis of BMI into normal weight (NW) and overweight or obesity (OW/OB).Serum RBP-4 was measured with ELISA;serum concentrations of hs-CRP,free fatty acids (FFA),triglyeride(TG),total cholesterol (TC) and insulin were measured in fasting status.Insulin resistance was assessed by HOMA-IR.ResultsLn(HOMA-IR) and the concentrations of FFA,ln(hs-CRP) in IGT or in GDM were significantly higher than that of NGT(P＜0.05).In the group of OW/OB,FFA[(0.90±0.31)mmol/L vs (0.71±0.28)mmol/L,t＝2.73,P＜0.05)],ln(hs-CRP)(0.62±1.00 vs -0.17±1.07,t＝2.90,P＜0.05),ln(HOMA-IR),lnRBP(0.56±0.27 vs 0.30±0.14,t＝2.86,P＜0.05)were significantly higher than that of NW.In the group of GDM,lnRBP-4 showed a positive relationship with FFA (r＝0.231,P＜0.05)and ln(hs-CRP) (r＝0.237,P＜0.05).There was a positive relationship between lnRBP-4 and FFA(r＝0.371,P＜0.05) or ln(hs-CRP)(r＝0.247,P＜0.05) in the group of OW/OB,but no association in the group of NGT,IGT or NW.There was no relationship between lnRBP-4 and ln(HOMA-IR) in IGT,GDM and NW,while in the group of NGT and OW/OB,lnRBP-4 showed a positive relationship with ln(HOMA-IR) (r＝0.276,0.290,P＜0.05).In a multiple linear regression analysis,ln(hs-CRP),FFA or ln(HOMA-IR) was not the independent factor of lnRBP-4 in any group.ConclusionsIn patients with gestational diabetes mellitus,lnRBP-4 may be a new marker of inflammation.

Objective To investigate the effect of psychological abuse in childhood on the personality of undergraduates with depression as well as its possible mechanism, and thus provide scientific evidence for the intervention of depression in undergraduates. Methods Particinants were 733 undergraduates from a university in Henan Province. They were surveyed with Center for Epidemiological Studies-Depression scale (CES-D), childhood experience of care and abuse questionnaire (CECA. Q) and Eysenck personality theory (EPQ). Results the correlation between maternal antipathy and introversion-extroversion of personality;psychological abuse in childhood had apparently positive correlation with the depression in undergraduates (r = 0. 257, r = 0. 304, r =0. 243, P＜ 0. 01) and that of childhood maternal neglect to psychoticism (β = 0.206, P＜ 0.01) were positive and significant. The standard regression coefficients of paternal neglect to introversion-extroversion (β= -0. 143, P＜0. 01)were negative and significant . The scores of childhood paternal antipathy and maternal antipathy explained that childhood paternal antipathy had indirect effect via adulthood neuroticism on the depression in undergraduates,furthermore, the indirect effect demonstrated 59.8% of the total effect. Conclusion This study suggests that the personality of undergraduates with depression is affected directly by psychological abuse in childhood, and adulthood personality may mediate the relation between childhood psychological abuse and adulthood depression.

Objective To investigate the effect of Akt on cardiomyocyte hypertrophy induced by hydrogen peroxide(H2O2).Methods The neonatal rat cardiomyocytes cultured in primary generation were treated with low concentrations of H2O2.The cardiomyocyte hypertrophy was evaluated by the determination of average cell volume and protein content.The effects of Akt inhibitor on cardiomyocyte hypertrophy induced by H2O2 was recorded.Western blot was performed to examine the phosphorylation of Akt induced by H2O2.ResultsH2O2 at 10 or 50 ?mol/L stimulated cardiomyocyte enlargement as measured by cell volume and the protein content per cell.The inhibitor of Akt inhibited the hypertrophic response of cardiomyoctes stimulated by H2O2.H2O2 increased the level of Akt phosphorylation in cardiomyocytes,while that is suppressed by the inhibitors of PI3K(phosphoinositide 3-kinase). Conclusion Akt signaling is involved in cardiomyocyte hypertrophy induced by H2O2.

Objective · To investigate the role of interleukin-10 (IL-10) in regulating ocular neovascularization (NV). Methods · Expression of IL-10 was investigated in mice with oxygen-induced retinopathy (OIR) and transgenic mice with VEGF expression in photoreceptors by immunofluorescence,RT-PCR, and Western blotting. Mice deficient in IL-10 were used to test the effect of IL-10 in retinal, sub-retinal, and choroidal NV. Results · In OIR mice and transgenic mice with VEGF expression in photoreceptors, the staining intensity and mRNA expression of IL-10 were increased. Mice deficient in IL-10 showed a significant reduction in ischemia-induced retinal NV, and choroidal NV at rupture sites in Bruch's membrane. Mice lacking IL-10 showed reduced levels of hypoxia-inducible factor-1α (HIF-1α) and suppression of ischemia-induced expression of VEGF and VEGF receptor 1. Macrophage was regulated and reduced in ischemic retina of mice with IL-10 deficiency. Conclusion · IL-10 stimulates ocular NV through modulation of HIF-1α and its target genes VEGF and VEGF receptor 1. IL-10 promotes ocular NV via macrophage response to retina ischemia.

Objective To investigate the qualitative diagnostic value of MRCP in patients with benign obstructive jaundice.Methods MRCP and conventional MR images were performed in 128 patients with benign obstructive jaundice on a superconductive MR scanner (Plilips medical systems Inc. Eclipse. 1.5T) . Final diagnosis was confirmed by surgical and histopathological findings in 123 patients and other 5 patients by follow-up.Results The accuracy of location diagnosis of MRCP was 100%; the qualitative diagnosis was 95.3%. The specificity, accuracy, sensitivity of the deadwood-sign for diagnosis of benign obstructive jaundice were 100%, 95.3%, 85.7% respectively. Conclusion The deadwood-sign was a specific indicator in MRCP of benign obstructive jaundice.

Objective We have examined tumor suppressor gene p53 from N- methyl - N- nitrosourea (MNU ) induced the col - orectal tumors in rats. Methods PCR, SSCP and DNA sequence analysis. Results In colorectal tumors induced by MNU, the mobil- ity shifts of p53 gene mutation were observed, p53 gene mutation was a deletion at third position C of codon 244 in exon7. Conclu- sion The present study suggested that MNU induces rat p53 mutations in the colorectal tumors.

Objective To study the role of miR-320-3p in adipocyte differentiation. Methods Marrow mesenchymal stem cells were isolated from mice and cultured then induced with adipogenic agents for 3 days. The transcription level of miR-320-3p was examined by qRT-PCR. Stromal ST2 cells were transfected with miR-320-3p, followed by adipogenic treatment. Oil-red O staining and qRT-PCR were employed to assess the differentiation of adipocytes induced by miR-320-3p. Results The expression level of miR-320-3p increased in MSCs after adipogenic treatment (P < 0.01). Addition of miR-320-3p in ST2 cells promoted the formation of oil-red O positive adipocytes and up-regulated the expression levels of adipogenic transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein-α(C/EBPα) and the marker gene adipocyte fatty acid binding protein 4 (FABP4),compared to cells that transfected with miR-320-3p mimics (P<0.05). Conclusion miR-320-3p promotes ST2 cells differentiation into adipocytes.

OBJECTIVE:To study sterility test after using Non-PVC bivalve soft-bag injection in PIVAS. METHODS:The test was divided into 3 groups according to the type of transfusion solution packaging and dispensing environment. Group 1 received Glucose solution using bivalve soft-bag,dispensed in PIVAS;group 2 received Glucose solution using bivalve soft-bag,dispensed in wards area;group 3 received Glucose solution using plastic bottle,dispensed in wards area. After puncturing 1,3,6,9 times (n=80),finished products placed in ward for 0,2,4,6 h(n=20),and then sterility test was conducted with membrane filtra-tion method stated in second part of Chinese Pharmacopoeia (2010 edition). Infusion contamination of 3 groups was analyzed at 9th puncture. RESULTS:The growth of bacteria was not found in group 1;the positive detection rate of group 2 and 3 were 2.5%and 3.8%(n=320). The total positive detection rates after puncturing 1,3,6,9 times were 0,0.4%,0.4%,7.5%(n=240);the positive detection rates of group 1 were all 0,those of group 2 were 0,1.25%,0,8.75%and those of group 3 were 0,0,1.25%, 13.75%(n=80). After 9 times of puncture,the positive detection rates of group 1 after placing 0,2,4,6 h were all 0,those of group 2 were 25%,5%,0,5%;those of group 3 were 5%,15%,5%,30%(n=20). CONCLUSIONS:The use of the Non-PVC bi-valve soft-bag injection in PIVAS can effectively prevent microbial contamination.

Aim Through identification of the difunc-tionality of neuroprotection and passage of blood-brain barrier ( BBB) for RDP-BDNF fusion protein to offer a new strategy to treat brain diseases. Methods BDNF was conjugated to a novel cell penetrating peptide known as RDP which was derived from rabies virus gly-coprotein ( RVG) . The fusion protein RDP-BDNF was expressed in E. coli BL21 ( DE3 ) . After the intrave-nous injection of RDP-BDNF, the time effect curve of RDP-BDNF in the brains and serum was examined. Additionally , Morris water maze test was used to evalu-ate the effect of RDP-BDNF on scopolamine-induced amnesic mice, and the mechanism was also studied. Results RDP-BDNF could cross the BBB and exhibi-ted neuroprotective effects on the treatment of cognitive deficit induced by scopolamine in dementia model mice. Conclusions Delivery of protein therapeutics using RDP might offer a new and exciting strategy to treat brain diseases.

The‘eighteen clashes’and‘nineteen fears’have been tought of traditional prohibited combination in long time,but many examples show ginseng and trogopterus dung could be safely used after compatibility,so many scholars fouce on the essential of compatibility.While people can not reach an accordant consideration for compatibility of Ginseng with Trogopterus Dung,therefore we have a overview based on documents for about 30 years,which will provide evidences for researching systematically on the nature of compatibility of Ginseng with Trogopterus Dung.