Objective To study the role of miR-320-3p in adipocyte differentiation. Methods Marrow mesenchymal stem cells were isolated from mice and cultured then induced with adipogenic agents for 3 days. The transcription level of miR-320-3p was examined by qRT-PCR. Stromal ST2 cells were transfected with miR-320-3p, followed by adipogenic treatment. Oil-red O staining and qRT-PCR were employed to assess the differentiation of adipocytes induced by miR-320-3p. Results The expression level of miR-320-3p increased in MSCs after adipogenic treatment (P < 0.01). Addition of miR-320-3p in ST2 cells promoted the formation of oil-red O positive adipocytes and up-regulated the expression levels of adipogenic transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein-α(C/EBPα) and the marker gene adipocyte fatty acid binding protein 4 (FABP4),compared to cells that transfected with miR-320-3p mimics (P<0.05). Conclusion miR-320-3p promotes ST2 cells differentiation into adipocytes.

Objective To investigate the application of fundamental cause in the preparation of surgical instruments.Methods The fundamental cause analysis method was used to analyze the situations of improper preparation of surgical instruments in the operation room to analyze the fundamental causes and work out countermeasures.The preparation of surgical instruments before and after the implementation was compared.Result The qualification rate of surgical instruments increased from 96.0％ before the implementation to 98.6％ after the implementation,with statistically significant difference (P＜0.001).Conclusion The fundamental cause analysis can help the operation room to find out the problems in the surgical equipment preparation process so as to improve the working procedures and the qualification rate of prepared surgical instruments.

Objective:To study the biotransformation rule of the chemical components of total ginsenosides from ginseng stems and leaves tranformed by submerged fermentation of ganoderma lucidum,and to lay the foundation for further development and research of the medicinal fungus of ginseng stems and leaves.Methods:The total ginsenosides from ginseng stems and leaves were fermented by ganoderma lucidum,the changes of chemical components before and after fermentation were measured by thin-layer chromatography;the levels of total ginsenosides from ginseng stems and leaves before and after fermentation were detected by ultraviolet spectrophotometry;the levels of ginsenoside Rb1,Rg3,Rd,CK,Re,Rg,and Rh1 before and after fermentation were measured by high performance liquid chromatography.The human hepatocellular carcinoma SMMC-7721 cells were divided into blank control group,low,medium and high doses of total ginsenosides from ginseng stems and leaves groups and medicinal fungus of ginseng stems and leaves groups.The dosages of total ginsenosides from ginseng stems and leaves and medicinal fungus of ginseng stems and leaves were 5,10 and 20 mg·L-1,respectively.The inhibitory rate(IR) of the cells was determined by MTT assay.Results:The results of thin-layer chromatography showed that the saponins before and after fermentation occured mutual transformation;the levels of total ginsenosides from ginseng stems and leaves before and after fermentation were 749.98 and 602.26 mg·g-1,respectively;the levels of panaxadiol saponins Rb1,Rg3,Rd and CK before fermentation were 24.54,2.21,87.22 and 0 mg·g-1,and the levels of panaxatriol saponins Re,Rg and Rh1 were 151.34,77.02,and 3.06 mg·g-1.After fermentation,the level of Rb1 was decreased by 61.45%,the levels of Rg3,Rd and CK were increased by 238.91%,34.43% and 268.00%.The levels of Re and Rg1 were decreased by 63.75% and 64.41%,and the level of Rh1 was increased by 408.88%;the IR of the SMMC-7721 cells in medium dose of medicinal fungus of ginseng stems and leaves group was higher than that in medium dose of total ginsenosides from ginseng stems and leaves group(P<0.05);the IR of the SMMC-7721 cells in high dose of medicinal fungus of ginseng stems and leaves group was higher than that in high dose of total ginsenosides from ginseng stems and leaves group(P<0.01).Conclusion:The total ginsenosides from ginseng stems and leaves transformed by the submerged fermentation of ganoderma lucidum can significantly change its chemical components,which have stronger anti-proliferation activity in the SMMC-7721 cells.

Objective To investigate the effect of dapagliflozin on vascular function and progres-sion of renal function in patients with diabetic nephropathy(DN).Methods A total of 122 DN patients were randomly divided into control group and observation group,with 61 cases in each group.The control group received conventional treatment,while the observation group received conventional treatment and dapagliflozin.Glycometabolism,vascular function,renal function indicators and adverse reactions were compared between the two groups.Results After treatment,there were no significant differences in fasting plasma glucose(FPG)and 2-hour postprandial glucose(2 hPG)between the two groups(P＞0.05),but the glycosylated hemoglobin(HbA1c)level in the observation group was sig-nificantly lower than that in the control group(P＜0.05).The flow-mediated dilation(FMD)for 1 minute at resting state and FMD for 5 minutes at ischemia state were significantly higher in the observa-tion group than those in the control group(P＜0.05);the estimated glomerular filtration rate(eGFR)and 24-hour urinary sodium level in the observation group were significantly higher than those in the control group,while the 24-hour urine protein level was significantly lower than that in the control group(P＜0.05).There was no significant difference in the incidence of adverse reactions between the two groups(P＞0.05).Conclusion Dapagliflozin can effectively alleviate vascular function damage and inhibit the progression of renal function damage in DN patients.

Objective To investigate the effect of dapagliflozin on vascular function and progres-sion of renal function in patients with diabetic nephropathy(DN).Methods A total of 122 DN patients were randomly divided into control group and observation group,with 61 cases in each group.The control group received conventional treatment,while the observation group received conventional treatment and dapagliflozin.Glycometabolism,vascular function,renal function indicators and adverse reactions were compared between the two groups.Results After treatment,there were no significant differences in fasting plasma glucose(FPG)and 2-hour postprandial glucose(2 hPG)between the two groups(P＞0.05),but the glycosylated hemoglobin(HbA1c)level in the observation group was sig-nificantly lower than that in the control group(P＜0.05).The flow-mediated dilation(FMD)for 1 minute at resting state and FMD for 5 minutes at ischemia state were significantly higher in the observa-tion group than those in the control group(P＜0.05);the estimated glomerular filtration rate(eGFR)and 24-hour urinary sodium level in the observation group were significantly higher than those in the control group,while the 24-hour urine protein level was significantly lower than that in the control group(P＜0.05).There was no significant difference in the incidence of adverse reactions between the two groups(P＞0.05).Conclusion Dapagliflozin can effectively alleviate vascular function damage and inhibit the progression of renal function damage in DN patients.