Objective:To analyze the relationship between types of Traditional Chinese Medicine(TCM)Syndrome of gastroesophageal reflux disease(GERD)、24-hour pH value in esophagus and its endoscopic performance in order to look for objective indexes for differentiation for syndrome classification in TCM of GERD.Methods:Among 172 patients finished 24hour pH dynamic monitor,Each patient performed upper gastrointestinal endoscopy to detect reflux esophagitis(RE)and grade the endoscopic findings,regulated common types of TCM syndrome of GERD,and relativity between then were compared.Results:All parameters of acid exposition in the type of heat stagnation in the liver and stomach were obviously higher than that in other tapes(P

0.05). Conclusions Radical TUVBT can be selected as surgical treatment for muscle-invasive bladder cancer cases,especially for those who are older and weak,and not medically fit for radical cystectomy or those who refuse the open surgery.

Objective To investigate the antiplatelet aggregation activity of clopidogrel ( small dose) combined with paeonol. Methods Antiplatelet aggregation activity was evaluated by platelet aggregation induced by adenosine diphosphate in vitro, the weight of arteriovenous bypass thrombosis in rats and bleeding time in mice. Results The combination significantly inhibited platelet aggregation, and reduced formation of arteriovenous bypass thrombosis as well as prolonged the bleeding time. Moreover, the activity of combined group was more superior to clopidogrel and paeonol, respectively. Conclusion The antiplatelet aggregation activity of the combination is better than clopi-dogrel, which has bright prospects in clinical applications.

Objective To investigate the feasibility of laparoscopic operations in treating large ovarian cysts.Methods A total of 38 patients with large ovarian cysts underwent laparoscopic cyst cystectomy or adnexectomy on the affected side from December 2002 to October 2005.The 2nd or 3rd trocar(usually on the tumor side)was inserted into the cyst,the fluid in which was drained with a suction unit.The cyst was then removed after being elevated toward the incision,which was extended to 20 mm.Results Large ovarian cysts in the 38 cases were all removed successfully,including cystectomy in 28 cases and adnexectomy in 10 cases respectively.No complication occurred.The follow-up period was 1-24 months(mean:10 months)for 35 cases.No abnormal findings in pelvic examination and B ultrasonography were observed.Conclusions Laparoscopic operation is a safe and feasible therapeutic method for large ovarian cysts.

Objective　To study the feature of immunoglobulin complementarity determining region 3 (CDR3) gene in neonates of different gestational age (GA), and the effect of neonatal maturity on the diversity of CDR3 nucleotide sequence. Methods　DNA were extracted from cord blood of 10 neonates of very immature (25-30 weeks), 12 immature (31-36 weeks), and 11 mature (37-41 weeks). CDR3 sequence was amplified suing nested PCR technique then cloned and sequenced. Results　1. There was a CDR3 length of 29.4±7.8, 32.4±9.2 and 40.8±10.7bp, including NDN length of 13.5±5.6, 16.1±7.8 and 22.0±8.5bp respectively in very immature, immature and mature neonates. 2. DP73 and DP75 were preferentially used in very immature and immature neonates, VH5, DP73 and DP75 were used in mature neonates. The usage rate of DP73 and DP75 reduced, whereas VH5 was raised with GA increasing. 3. For D gene segment, there was a frequent use of DN, DQ52 and DXP in very immature neonates, whereas DXP, DLR and DN in immature and mature neonates. 4. JH4 usage was preferential, followed by JH6 in all neonates, and their usage rate was decreased with GA increasing. 5. 63.3%, 68.8% and 92.0% of CDR3 sequence respectively in very immature, immature and mature neonates have an open reading frame with 303bp long encoding 101 residues. Conclusion　During the early life of neonates, VH-D-JH rearrangement of IgH gene is in an active condition, but the diversity is limited. Humoral immunity is a gradual development with increasing GA. Both heterogeneity and similarity of CDR3 sequence exists in neonates with different GA.

Objective To investigate the relationship among IGF-II expression and cell proliferation and apoptosis in human colorectal adenocarcinoma. Methods 60 paraffin embedded samples of human colorectal adenocarcinomas were used in this study. IGF-ⅡmRNA expression was detected by in situ hybridization, proliferation cell nuclear antigen(PCNA) protein was measured by immunohistochemical staining and apoptosis was determined by TUNEL technology. Fifteen normal colorectal tissues were used as controls. Computerized image analysis system qualitatively analyzed the positive ratio of above three indices in the cancer and normal tissues. Results The percentage of IGF-ⅡmRNA expression was significantly higher in colorectal adenocarcinoma (39.64%?7.32%) than that in normal colorectal tissues (23.21%?4.10%) (P

Objective To investigate the inhibitory effect of maspin gene on metastasis and invasion of gastric cancer and its mechanism.Methods Maspin gene was ligated to the expression vector PCR2.1 with T4 DNA ligase after the PCR2.1 was digested by HindⅢ/XbaⅠ.The recombinant vector maspin/PCR2.1 was transfected into human gastric cancer cell lines MKN28 and SGC7901,then the mRNA and protein expression changes of maspin gene,uPA and uPAR were detected respectively by PT-PCR and Western blotting.Results After identified by digestion and sequencing,the reconstructed plasmid was confirmed to contain the correct and full nucleotide sequence of maspin gene.The expressions of maspin gene were up-regulated in MKN28 and SGC7901 cell lines,while the expressions of uPA and uPAR were down-regulated.Conclusion The expression vector maspin/PCR2.1 is constructed successfully and can be expressed in eukaryotic cells.Expressions of uPA and uPAR can be inhibited by maspin gene.

Objective To construct a recombinant eukaryotic vector expressing maspin cDNA,and to explore the effect of maspin overexprssion on the proliferation of human gastric carcinoma cell line SGC7901.Methods The fragment of maspin gene was amplified by polymerase chain reaction(PCR).An eukaryotic vector expressing maspin(maspin/PCR2.1)was constructed with PCR2.1,and transfected into SGC7901 cells.The expression of maspin at mRNA and protein level was detected by RT-PCR and Western blotting.The proliferation of SGC7901 cells was observed by MTT.Cell cycle was analyzed by flow cytometry.Results Recombinant plasmid maspin/PCR2.1 was constructed and transfected into SGC7901 successfully.The mRNA and protein levels of maspin were significantly higher in the maspin/PCR2.1 group(33.6?1.2,23.4?1.6)than that in the blank PCR2.1(15.0?1.5,12.3?1.5)and the untreated group(13.7?2.0,12.0?1.3)(P

AIM: To study the effect of shenmai injection (SMI), a Chinese medicine, on nitric oxide and the expression of endothelial nitric oxide synthase (eNOS) mRNA in myocardium of rats with experimental myocardial ischemia. METHODS: Rats were randomly divided into four groups: control, model (ischemia), SMI 1 and SMI 2 group. A rat model of acute myocardial ischemia was established by isoprenaline treatment and the lift of ST segment in ECG was used as the index of myocardial ischemia. The nitric oxide (NO) contents in serum and myocardium and the expression of eNOS mRNA in myocardium were measured. RESULTS: Compared with control group, ST segment of ECG was significantly elevated 20, 30, 40 min after myocardial ischemia in model group, the lift peak of ST segment occurred 20 min after myocardial ischemia, the concentration of NO in the serum and myocardium and the expression of eNOS mRNA in myocardium were significantly lowered in model group. Compared with the model group, in SMI 1 group and SMI 2 group, the concentration of NO in the serum and myocardium and the expression of eNOS mRNA in myocardium were significantly increased, the lift of ST segment were significantly reduced 20, 30, 40 min after myocardial ischemia. Compared with SMI 1 group, the concentration of NO in the serum and myocardium and the expression of eNOS mRNA in myocardium and the lift of ST segment were not statistically different in SMI 2 group. CONCLUSION: Shenmai injection can increase the expression of eNOS mRNA in myocardium and the content of NO, and protect against myocardial ischemia in rats.

Following the requirements for libraries in the Implementing Rules for Evaluation Standards of Tertiary Hospitals in Guangdong Province and taking municipal or above occupational disease prevention and control institutions as objects,the paper analyzes the construction situations,current collections and operating modes of libraries and discusses the setting of the occupational health information service function from 6 aspects of information sources,service objects and function setting,book retrieval,inter-library loan,service feedback and occupational health information service for labor.