1.Practice and exploration on teaching reform of laboratory animal science in medical universities
Chinese Journal of Comparative Medicine 2017;27(3):93-95
Laboratory animal science, as a supporting discipline in life science research, has been set up courses in many universities, especially in medical schools.However, due to the curriculum design was too rigidly attached to tradition, emphasis on theory and cannot adapt to the development of natural science.In this paper, aiming to enhance the teaching effect, we focus on the practice, advance with the times, combine with customized teaching reform of laboratory animal science curriculum, teaching content and teaching forms.
2.Arsenic exposure and skin cancer
Chinese Journal of Endemiology 2017;36(1):74-78
Arsenic is a common environmental toxin,but also a carcinogen.Long-term exposure to inorganic arsenic can cause multi-system and multi-organ damage in the body,including cancerous and non-cancerous lesions.As one of the main target organs of arsenic exposure,skin damage is of great significance for the diagnosis and health assessment of arsenic poisoning in population threatened by the disease.From the perspective of arsenic exposure and skin cancer,the aim of this article is to summarize the epidemiology,pathogenesis and medical intervention of arsenic-induced skin cancer,and to provide reference for the pathogenesis and prevention of endemic arsenic poisoning related skin diseases.
3.Determine the polymorphisms and parent′s gene-sharing rate of LMP2 in women with pre-eclampsia and the relationship with severe pre-eclampsia
Aihua WU ; Meiyang GAO ; Linjuan ZENG
Chinese Journal of Perinatal Medicine 1998;0(03):-
Objective To study the polymorphisms of the large mutiplefunctional protease (LMP2) genes in women with severe pre-eclampsia (S-PE) and the sharing rate of LMP 2 in these women and their partners and couples. Methods Totally, 102 S-PE women and their husbands were enrolled in the study group, and 200 normal pregnant women and their husbands as control. The types of LMP2 alleles were assessed by polymerase chain reaction-restriction fragment lengthpolymorphism method (PCR-RFLP) after PCR. Results The frequency of LMP2R/H in S-PE group was significantly higher than that of the control (53.9% vs 35.%, P
4.Study on the Contents of Naringin in Fructus Aurantii Immaturus of Different Specifications
Aihua HUANG ; Haifeng CHENG ; Yuaner ZENG ;
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(02):-
Objective To study the content difference of naringin in Fructus Aurantii Immaturus of different specifications.Methods HPLC was performed on the Merck- Lichrospher RP-C18(4.6 mm? 250 mm, 5 ? m) with temperature of 35 ℃ .The chromatographic conditions for naringin:the mobile phase consisting of acetonitrile-0.3 % Phosphoric acid(20 ∶ 80), at the flow rate of 1 mL/min, and the detection wavelength at 283 nm. Results The content of naringin increased with the diameter of Fructus Aurantii Immature slices.Conclusion It is suggested that there exists significant differences in intrinsic quality among Fructus Aurantii Immaturus of different specifications.
5.Medical ethics in medical universities and colleges is the essential component of quality education for students of medical sciences
Yanping ZHAO ; Aihua ZENG ; Weihua HUANGFU
Chinese Journal of Medical Education Research 2005;0(06):-
It is suggested in this article that medical ethics should be conceived as the integral part of quality education for students of medical sciences,based on the high consistency of medical ethics with the quality education in higher educational institutions and its close relationship with the cultivation of the qualities of medical students.
6.Unilateral olfactory functional deprivation model in the left peripheral nostrils by electric cautery injury:olfactory bulb neurogenesis and transformation
Jianjun ZENG ; Xu HE ; Xiaoxin YAN ; Xuegang LUO ; Aihua PAN
Chinese Journal of Tissue Engineering Research 2014;(2):231-238
BACKGROUND:Olfactory bulb neurogenesis, transformation and maturation have been considered the hot topic. Olfactory bulb experience and nervous activity can influence olfactory bulb neurogenesis. However, no study reports that olfactory bulb functions can affect olfactory bulb neurogenesis in guinea pigs.
OBJECTIVE:To investigate the effect of unilateral olfactory functional deprivation on doublecortin, calbindin and parvalbumin expression in olfactory bulb of juvenile guinea pigs.
METHODS:Total y 24 guinea pigs were randomly divided into two groups, which were kil ed after establishing olfactory functional deprivation model through electric cautery injury at the left peripheral nostrils. At 3 and 6 weeks after modeling, the specimens were harvested. The expression change of doublecortin, calbindin and parvalbumin in two sides’ olfactory bulb of juvenile guinea pigs was detected by immunohistochemistry.
RESULTS AND CONCLUSION:The number of doublecortin, calbindin and parvalbumin positive cells in olfactory bulb at the un-deprived side was significantly higher than that at the deprived side at 3 and 6 weeks (P<0.05). This finding indicates that olfactory neural activities can affect neurogenesis and transformation in guinea pigs.
7.Lymphocyte immunological function and 24 T cell receptor V beta subfamilies expression in convalescent patients with severe acute respiratory syndrome
Xing ZENG ; Cui CAI ; Yu HUANG ; Aihua OU ; Xian ZHANG
Chinese Journal of Tissue Engineering Research 2007;11(43):8796-8800
BACKGROUND: Severe acute respiratory syndrome (SARS) is caused by a genetically novel coronavirus that is caused by acute infectious disease. It is not yet clear for the immunology function of SARS patients in their convalescent stage.OBJECTIVE: To study the effects on T lymphocyte, and the titer profiling of 24 T cell receptor (TCR) V β subfamilies expressions in SARS convalescent patients.DESIGN: A self-control observation.SETTING: Central Laboratory, Guangdong Provincial Hospital of Traditional Chinese Medicine.PARTICIPANTS: Seventy-six cured SARS patients who received treatment in the Second Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine between January and April 2003. All the patients corresponded to "clinical diagnostic criteria of atypical pneumonia", " diagnostic criteria of severe atypical pneumonia and discharge criteria" and "clinical diagnostic criteria and discharge criteria of severe acute respiratory syndrome". The involved patients, 30 male and 46 female, averaged (32±11 (years old. Another 10 subjects who simultaneously received health examination in the same hospital, 5 male and 5 female, aged (32±7(years, were involved in the study. Informed consents of detected items were obtained from all the subjects.METHODS:①Detecting the expression of 24 T cell receptor(TCR)V β subfamilies in SARS convalescent patients:Peripheral blood(2 mL) was collected from the healthy convalescent subjects,and EDTA-K2 was used as anticoagulant.In the flow cytometry delection tubes.10 μL various fluorescein-labeled mAb,such as anti-CD3,anti-CD4,anti-CD8,anti-CD25,anti-CD28,anti-HLA-DR,anti-CD3mAb conjugated with PC5,TCR Vβ1(PE+FITC).Vβ2(PE+FITC)。Vβ3 (FITC),Vβ4(PE+FITC),Vβ5.1(PE+FITC),Vβ5.2(PE),Vβ5.3(PE),Vβ7.1(PE+FITC),Vβ7.2(FITC),Vβ8(FITC),Vβ9 (PE),Vβ11(PE),Vβ12(FITC),Vβ13.1(PE),Vβ13.2(PE),Vβ13.6(PE+FITC),Vβ14(FITC),Vβ16(FITC),Vβ17 (PE+FITC),Vβ18(PE),Vβ20(FITC),Vβ21.3(FITC),Vβ22(PE+FITC)and Vβ23(PE),was added in special flow tubes,and then 50 μL whole blood was added.The mixed solution was incubated away from light for 15 minutes.After erythrocytolysin being added,mixed solution was washed.Finally.cell deposit was dissolved in 300 μl phosphate buffer solution (PBS).Coulter ESP flow cytometer was used for detection.For the analysis of TCR expression,an electronic gate was set on these cells and at least 5000 events per sample were collected.Three-color cytofluodmetric analysis was performed using a Coulter ESP flow cytometer.②Detecting the T cell subset,activated T and B cells,and the percentage of Ts and Tc cells:5000 cells were collected and used to calculate the expression of T cells (CD3,CD4 and CD8),the activated T and B cells(CD3+/CD25+,CD3+/HLA-DR+ and CD3-/HLA-DR+),as well as the percentage of Ts and Tc cells by Coulter ESP flow cytometer and its software.MAIN OUTCOME MEASURES:①The change of T cell subset(CD3,CD4,and CD8)from SARS convalescent patients.②The change of activated T and B cells(CD3+/CD25+,CD3+/HLA-DR+ and CD3-/HLA-DR+).③The percentage of Ts and Tc cells(CD8+/CD28+,CD8+/CD28-)in convalescent patients.④Analysis of the 24 TCR V β subfamilies from SARS patients in convalescence.RESULTS:All data were explored to analyze the expression profiling of 24 TCR Vβ subfamilies,the data from 74 SARSpatients and 10 healthy controls were explored to other result analysis.①The detecting results of T celI subset:The percentage of CD4+T cell mean value was lower than the reference value[(33.33±6.64)% vs.(43±9)%,P<0.01].The percentage of CD8+T cell mean value was higher than the refefence value[(34.07±6.40)% vs.(30±9)%,P<0.01].② The expression of activated T and B cells:Percentage of HLA-DR+ T and B cell was Increased while the percentage of CD25+ T-cell was decreased compared with reference values.In 53 out of 74 patients,the percentage of CD25+ T cells was lower than the reference value,and 64 patients had a lower percentage in CD3+/CD25+ T cells.The percentages of CD3+/HLA-DR+ and CD3-/HLA-DR+ cells were higher than the normal reference value.T cells expressing higher CD3+/HLA-DR+ were found in 36 patients,and T cells expressing higher CD3-/HLA-DR+ were found in 30 patients.③The ratios of Ts and Tc cells:The percentage of Ts cells which expressed CD8+/CD28- was increased compared with reference value [(28.75±7.31)% vs.(15.99±5.1)%,P<0.01],while the percentage of Tc cells which expressed CD8+/CD28+ was decreased [(5.99±3.60)% vs.(13.2±4.1)%,P<0.01].Thirty-nine patients were found to possess the lower Tc cells and forty-eight patients were found to possess the higher Ts cells.The ratios of both CD4+ and CD8+ T cells were in the normal reference value.④24 TCR Vβ subfamilies expressions in T cells:It was noteworthy that Vβ14 had a highest percentage in all 24 Subfamilies,and followed by Vβ 5.3,and Vβ 23 in the convalescent patients.The percentage of Vβ 14 was the highest in the normal controls,which was consistent with the results of SARS patients.But the other subfamilies expression patterns were different.There were significant differences between Vβ1,Vβ5.2,Vβ5.3,Vβ7.2,Vβ9,Vβ11,Vβ13.1,Vv13.2,Vβ17,Vβ18,Vβ22 and Vβ23.In the convalescent period,each TCR Vβ expression of SARS patients was higher than that of controls(P<0.05-0.01).CONCLUSION:In SARS convalescent patients,the increased CD8+CD28- T cell may elevate CD8+ T cell number;Meanwhile.the reduced CD3+ and CD4+ T cell number may be corresponding to the increased Ts cell number.For some inhibiting factor secreted by Ts cell was also increased.The usage pattern of 24 TCR Vβ subfamilies in SARS patients is different from that of control group.The increase of percentage of CD3+/HLA-DR+ and CD3-/HLA-DR+ T cell may be related to the late response of activated T and B cells.
8.Study on Selection of Preparation Procedure of Buqiyangxuecuiru Oral Liquid
Hua YU ; Xianyi ZENG ; Lu FANG ; Aihua ZHANG ;
Chinese Traditional Patent Medicine 1992;0(12):-
Objective: To select the optimum extraction procedure for Buqiyangxuecuiru Oral Liquid. Methods: The water extraction, water extraction and alcohol precipitation, and alcohol percolation procedures were designed. Taking contents of ferulic acid and astragalus saponins I, and the lactation quantity of normal lactation mice as markers, the rational preparation procedure was selected. Results: The oral liquid prepared by the procedure of alcohol percolation. Both ferulic acid and astragalus saponins I contents in this preparation were the highest, and it could remarkably increase the lactation quantity of normal mice. Conclusion: The alcohol percolation procedure was the optimum extraction procedure for Buqiyangxuecuira Oral Liquid.
9.Optimization of Extraction Technology for Neohesperidin in Fructus Aurantii Immaturus by Orthogonal Experiment
Aihua HUANG ; Yuan'Er ZENG ; Yuguang CHI ; Haifeng CHENG ;
Traditional Chinese Drug Research & Clinical Pharmacology 2000;0(06):-
Objective To optimize the extraction process of neohesperidin in Fructus Aurantii Immaturus.Methods The optimum proess was investigated by L9(34) orthogonal design with the content of neohesperidin as the observation index.Results Extraction times were the main factors for neohesperidin extraction.The optimal process condition was as follows:adding 6-fold 70 %ethanol,extracting for 3 times,one hour for each extraction.Conclusion The optimal extraction technology of neohesperidin in Fructus Aurantii Immaturus is stable and reliable.
10.Biomarkers in rats for kidney damage characteristics of arsenism due to coal burning and benchmark dose analysis
Yuyan XU ; Aihua ZHANG ; Jun LI ; Liyuan CHEN ; Maolin YAO ; Chun YU ; Qibing ZENG ; Jiang HE
Chinese Journal of Pharmacology and Toxicology 2014;(2):243-247
OBJECTIVE Study the kidney toxic effects caused by burning coal endemic arsenism in rats,application bench mark dose (BMD) method to investigate the bench mark dose of urinary arsenic (UAs)and the changes in bio markers of renal function.METHODS Wistar rats were fed for 90 d with arsenic 0,25,50,100 mg·kg -1 conta minated feed.Urinary arsenic,kidney arsenic and renal function indicators were determined,and routine pathological and fibrosis of kidney were exa mined.UAs as the exposure bio marker,Uβ2-MG,UNAG and UALB for the effect bio markers,application bench mark dose method to calculate the BMD and BMDL of UAs for each effect bio markers.RESULTS UAs,KAs, Uβ2-MG,UNAG,UALB levels of rats in arsenic 100 mg·kg -1 group were increased than normal group (P <0.05);In light microscope,the results of HE staining of rat kidney in all arsenic dose groups showed infla mmatory cell infiltration,renal tubular epithelial cell swelling,renal interstitial capillary dila-tion,congestion and other varying degrees pathological changes,and the results of masson staining showed varying degrees of tubulointerstitial fibrosis;UAs as the exposure bio marker,Uβ2-MG,UNAG, UALB for the effects of mark,the BMD and BMDL of UAs for Uβ2-MG,UNAG,UALB were calculated, the BMD values were 998.9,1213.5,1386.9 μg·g -1 Cr,the BMDL values were 660.5,803.6 and 909. 4 μg·g -1 Cr,respectively.CONCLUSION Burning coal arsenic pollution can cause kidney da mage in rats,mini mal change nephropathy may be the pri mary pathological in the coal arsenic conta mination of kidney da mage.The BMD and BMDL of UAs were 998.9,660.5 μg·g -1 Cr,the early changes of renal function of burning coal arsenism in rats;it is reco mmended to use the more sensitive bio markers Uβ2-MG to calculate the biological exposure li mits on renal injury caused by arsenic.