Objective To study the effect of acupuncture and medication on plasma ealcitonin gene-related peptide(CGRP) levels in patients with acute cerebral infarction. Methods 90 patients with simple obesity were ran-domly divided into acupuncture and medication group( n = 45) and medication group( n = 45 ). All patients were ex-amined the plasma level of CGRP to observe clinical curative effect before and after 1 month treatment. Results The total effective rate of acupuncture and medication group 91.1% (41/45) was significantly higher than that in medication group 77.8 % (36/45) (P < 0.05) ; after treatment the plasma level of CGRP in two groups were signifi-candy higher than those before treatment(P < 0.05) , acupuncture and medication group was significantly higher than that in medication group (P < 0.05). Conclusion Acupuncture in combination with drug could cut down nerve cell damage, and it may be significantly related to levels of CGRP in patients with acute cerebral infarction.

Aim To observe the effect of Ginkgo biloba extract 50(GBE50)on L-type calcium current and cytosolic[Ca~(2+)]_i in ischemic guinea pig ventricular myocytes.Methods Single ventricular myocytes were isolated by enzymatic dissociation. I Ca-L was recorded by whole-cell patch clamp technique in voltage clamp mode.[Ca~(2+)]_i was detected by laser confocal micros-copy and represented by relative fluorescent intensity (FI).Results During ischemia, the peak Ca~(2+) current was reduced, and the I-U curve of I Ca-L was shifted upward.50 mg·L~(-1) GBE50 reversed the change induced by ischemia(n =6, P >0.05).After perfusing ischemic solution for 12 min, intracellular calcium concentration was increased(n =10, P <0.01).After perfusion with ischemic solution containing 50 mg·L~(-1) GBE50, the increase of intracellular calcium concentration was markedly inhibited(n =10, P >0.05).Conclusion GBE50 can reverse the decrease of I Ca-L and partially inhibit calcium overloading during ischemia.

With use of ~(125)I-udR release test,a significant natural killer activityagainst K_(562) target cells has been determined in human peripheral wholeblood(WB).r There was a gradual decrease in NK activity as rise inthe dilution or WB.In comparison with peripheral monouclear cells(PBMC)oneself,the NK activity lowered distinctly at both 1:4 and 1:8 dilution ofWB(p0.4).It seems that neither the red blood cells nor thePlasma ffeect on NK activity of WB,The NK activity in WB as well as inPBMO was improved by incubation with human leukocyte interferon.TheNK activity of WB and PBMC in patients with hepatocellular carcinomawas signiticantly lower than in normal controls.The results showed tahtNK activity may be determined by using properly diluted WB instead orPBMC,and the assay can be easily performed in clinical practice.

Objective To evaluate the clinical significance of the changes of plasma B-type natriuretic paprid and NT-pro BNP levels in patients with chronic heart failure(CHF).Methods 120 consecutive patients hospitalized for CHF were retrospectively studied.NT-pro BNP and LVEDD,LVESD,LVEF were measured and compared in 120 patients and 50 normal control subjects before medical treatment and on discharge.Results The plasma NT-pro BNP levels in patients with CHF were significantly higher than controls( P <0.05).The plasma NT-pro BNP levels in patients with cardiac function NYHA Ⅰ were significantly higher than controls( P < 0.05).As the cardiac function deteriorated from NYNA Ⅰ to NYNA Ⅳ,the NT-pro BNP levels increased consecutively with significant differences from each other ( P < 0.05).The plasma NT-pro BNP levels was low when CHF was cured ( P < 0.05 ).Conclusion Determination of plasma NT-pro BNP levels in patients with CHF were helpful to study the severity and prognosis of disease.

Objective To know the influence of comfortable nursing for paitnets with malignant tumor on promoting their quality of life. Methods Selected 995 patients with malignant tumor who had diagnosed by pathology and coured by chemotherapy or radioehemotherapy. Divided them into the experimental group (498 cases) and the control group (497 cases) randomly. Routine nursing cares were used in the control group,while the comfortable nursing cares were used in the experimental group in addition. QLQ-C30 was used to evaluate the level of patients'quality of life at the time of beginning, ending and the midpoint of the treatment. Results There was no significant difference between the two groups about the QLQ-C30 values at the beginning of the treatment, while the differences were signifi-cant at the other two time points, the level of quality of life in the experimental group was better than in the control group. Conclusions Comfortable nursing can promote quality of life of patients with malignant tumor, which should be developed in the clinical field.

Objective To evaluate therapeutic effect and safety of hysteroscope operation in treating hysteromyoma. Methods 146 patients were subjected to transcervical resection of myoma (TCRM), curative effects were evaluated as satisfaction or dis-satisfaction according to the degree of bleeding control, the rate of growth of myoma and the need of subsequent surgery. Results The total satisfactory rate is 95%. and satisfactory rate of type 0 and Ⅰ reached 100%. There was no complication in all patients. Conclusion TCRM is the best choice in treating submucous myoma. The results of surgery were satisfactory to intramural myoma if gasp the indictions. Selecting proper indications for operation, B ultrasonic monitoring severity are important for ensuring the operation effect and safety.

Objective To investigate the prevalence of human immunodeficiency virus (HIV) infection and its characteristics among pregnant women in Hunan.Methods Data from information system of prevention of mother-to-child transmission of HIV management in Hunan 2011-2015 was analyzed in the study (3 + 1 mode by year statistics).Results The total HIV-positive infection rate was 0.19‰ among pregnant women from 2011 to 2015 in Hunan.The rate of HIV infection showed upward trend by years (P ＜ 0.05).The proportion of diagnosis of HIV positive cases intrapartum was 44.66％,showed declining trend by years (P ＜ 0.05).The 786 cases of HIV positive pregnant women were mainly the Han's,the age distribution of 20 to 35 years old,90.21％ of them were farmers or unemployed.A percentage (76.84％) of them had junior high school education level or lower 37.91％ of them were found in delivery or postpartum.A percentage (36.51％) of them accepted service in delivery or postpartum.A percentage (45.67％) of them was infected through sexual contact,46.82％ of them were infected by unknown ways.Conclusions The rate of HIV infection among pregnant women was increased by years in Hunan.It is suggested to strengthen health education among high-risk groups and high incidence areas,improve detection rate of early pregnant women,implement the Prevention of Mother to Child Transmission (PMTCT) measures to reduce the rate of mother to child transmission of Acquired Immune Deficiency Syndrome (AIDS).

OBJECTIVE:To evaluate the efficacy of latanoprost for patients with refractory angle-closure glaucoma in whom the intraocular tension failed to be kept under control by common anti-glaucoma drugs.METHODS:A total of 23 refractory glaucoma cases(36 eyes)whose intraocular pressure(IOP)failed to be kept under control by common anti-glaucoma drugs were assigned to receive latanoprost alone or latanoprost combined with timolol or pilocarpine for 6 months.The intraocular tension,visual acuity,visual field and side-effect were observed.RESULTS:In acute primary angle-closure glaucoma group,the mean IOP was(27.13?5.21)mmHg before treatment vs.(16.21?3.45)mmHg at six months(P

Objective To study the relationship between the invasion of pituitary adenomas and expressions of MMP-9,Ki-67 and nm23. Methods The expressions of MMP-9,Ki-67 and nm23 were examined by immunohistochemical Elivision methods in 78 cases of pituitary adenomas. Of them,40 cases were invasive pituitary adenomas and, 38 cases were noninvasive pituitary adenomas. Results The expression levels of MMP-9 and Ki-67 in the invasive pituitary adenomas were significantly higher than those in the noninvasive ones (P

Objective To investigate the association of anti-C1q antibody with disease activity and lupus nephritis in patients with systemic lupus erythematosus(SLE). Methods The serum Level of anti-C1q antibody was determined in 35 patients with SLE by ELISA. The correlation between anti-C1q level and other disease activity parameters, such as SLEDAI score, anti-dsDNA antibody, C3, C4 and CH50 levels, was analyzed. Results Anti-C1q antibody was positive in 51.4% of patients with SLE, the positive rates in patients with active nephritis and without one was 77.8%(14/18) and 42.3%(22/52), respectively, and that was significantly higher in the former than in the later(P