1.Decreasing bleeding effect of transamin on the induced abortion
Zirong HUANG ; Aihua FANG ; Zhongying DAI
Chinese Journal of Obstetrics and Gynecology 2001;0(07):-
0 05) between the two groups in the patient′s age, pregnancy age, parities, the cavity volume of uterine, hemoglobin and blood platelet count The bleeding volume was (51 6?17 2) ml for the treated group and (63 3?17 1) ml for the control group ( P 2 months It could decrease the volume of bleeding during the operation and benefit for patients recovering
2.Detection of Common Fungal Pathogens by Genenral Primer PCR
Min JIN ; Aihua HUANG ; Zhaoli CHEN
Journal of Environment and Health 2007;0(11):-
Objective To develop the detection method of common fungal pathogens by general primer PCR.Methods The primers were designed from the target genes of 5.8S rDNA and 28S rDNA of fungi,and the specificity and sensitivity were observed.Results The general primer PCR can be used to detected C.albicans,C.parapsilosis,C.krusei,C.glabrata,C.tropicalis,C.neoformans,A.fumigatus,A.flavus,A.nidulans,A.niger,C.carrionii,P.verrucosa,S.schenckii,F.pedrosoi,T.rubrum,T.mentagrophytes,M.gypseum,M.canis,E.floccosum,M.racemosus,the sensitivity was 15 pg/ml of DNA.Conclusion The general primer PCR can be used to detect common fungal pathogens.
3.Hypermethylation and Transcription and Expression of O~6-methylguanine-DNA Methyltransferase Gene in Patients of Endemic Arsenism
Xueli PAN ; Aihua ZHANG ; Xiaoxin HUANG
Journal of Environment and Health 1992;0(04):-
0.05),but the average levels of DNMT1 mRNA of mild and moderate groups were significantly lower than that of the control group(P
4.Relationship Between the Invasion of Pituitary Adenomas and Expressions of MMP-9,Ki-67 and nm23
Aihua LIU ; Wei HUANG ; Shaowen XIAO
Journal of Chinese Physician 2001;0(04):-
Objective To study the relationship between the invasion of pituitary adenomas and expressions of MMP-9,Ki-67 and nm23. Methods The expressions of MMP-9,Ki-67 and nm23 were examined by immunohistochemical Elivision methods in 78 cases of pituitary adenomas. Of them,40 cases were invasive pituitary adenomas and, 38 cases were noninvasive pituitary adenomas. Results The expression levels of MMP-9 and Ki-67 in the invasive pituitary adenomas were significantly higher than those in the noninvasive ones (P
5.Study on the Contents of Naringin in Fructus Aurantii Immaturus of Different Specifications
Aihua HUANG ; Haifeng CHENG ; Yuaner ZENG ;
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(02):-
Objective To study the content difference of naringin in Fructus Aurantii Immaturus of different specifications.Methods HPLC was performed on the Merck- Lichrospher RP-C18(4.6 mm? 250 mm, 5 ? m) with temperature of 35 ℃ .The chromatographic conditions for naringin:the mobile phase consisting of acetonitrile-0.3 % Phosphoric acid(20 ∶ 80), at the flow rate of 1 mL/min, and the detection wavelength at 283 nm. Results The content of naringin increased with the diameter of Fructus Aurantii Immature slices.Conclusion It is suggested that there exists significant differences in intrinsic quality among Fructus Aurantii Immaturus of different specifications.
6.The association of the expression level of anti-apoptosis protein p-AKT and gastric mucosal injury induced by indomethacin in mice
Ming GAO ; Aiguo TAN ; Aihua LIU ; Chunmei ZHAN ; Cibo HUANG
Chinese Journal of Rheumatology 2008;12(8):560-562,插三
Objective To investigate the association of the expression level of anti-apoptosis protein p-AKT and gastric mucasal injury induced by indomethacin in mice.Methods The cytotoxicity induced by indomethacin was measured by LDH assay.The p-AKT expression levels were measured in the gastric mucosal tissues from C57BL/6 mice and rat gastric mucosal (RGM-1) cell lines treated with indomethacin lay western blotting.Results The cytotoxicity induced by indomethacin was in a dose dependent manner.Compared with the control,a typical histological appearance of gastric ulcer was observed in the gastric mucosa of in domethacin-administered mice;p-AKT protein expression in the gastric mucosa of mice and RGM-1 cell lines was decreased after treated with indomethacin.Conclusion The reduction of Anfi-apoptesis protein p-AKT expression may be a new mechanism for the gastric mucosal injury induced by indomethacin.
7.Effects of low frequency electric stimulation at bilateral mastoid processes on motor function and cerebral blood flow of children with cerebral palsy
Guoxiang PANG ; Guanzhong HUANG ; Aihua ZHENG ; Feiyan LI
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(02):-
Objective To explore the effects of low frequency electric stimulation at bilateral mastoid processes on motor function and cerebral blood flow of children with cerebral palsy (CP). Methods Ninety children with CP were randomly divided into two groups: an electric stimulation group (group 1) and an conventional rehabilitation control group (group 2), thirty healthy children served as the normal control group. The children of group 1 were treated with FES in addition to the routine rehabilitation treatment. The children of group 2 were given of the routine rehabilitation treatment only. All the patients were treated successively with the above protocol for 3 months. The clinical effect and motor development were evaluated with the gross motor function measure (GMFM), and the blood flow velocities of anterior cerebral artery (ACA), middle cerebral artery (MCA), posterior cerebral artery (PCA) were measured by transcranial doppler (TCD) ultrasound before and after treatment. Results In group 1, significant or some improvement were achieved in 19 and 24 CP children, respectively, with an effective rate of 95.6% . In group 2, significant or some improvement were achieved in 10 and 27 CP children, respectively, with an effective rate of 82.2%. There was significant difference between the two groups with regard to the significant effective rate (P
8.Increased expression of Zbtb7 mRNA in leukemia cell lines induced by PMA
Lifang WANG ; Zhibo HAN ; Ying HUANG ; Peng DU ; Aihua SUN
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(03):-
Objective To detect the expression of Zbtb7 gene in Phorbol esters(PMA) induced differentiation of leukemia cell lines.Methods 50 nmol/L PMA was used to induce the differentiation of U937 and K562 cell lines.The expression of Zbtb7 was detected by real-time PCR in the cells after induction on day 0,1,3 and 5,respectively. Results The expression of Zbtb7 in leukemia cell lines was markedly enhanced after treated by PMA(P
9.A study on clinic manisfestation and serological character of late-onset systemic lupus erythematosus
Aihua LIU ; Yingjuan CHEN ; Chunmei ZHANG ; Qian WANG ; Cibo HUANG
Chinese Journal of Geriatrics 2003;0(07):-
Objective To observe the clinic features and serology of systemic lupus erythematosus(SLE) in elderly patients. Methods The clinic features and serology of 27 patients with late-onset SLE in our hospital from 1994 to 2003 were analyzed and compared with non-lateonset SLE. Results The average duration from disease onset to diagnosis in late-onset SLE was 56.1 months, which was significently longer than in non-lateonset SLE (mean 10.5 months, P
10.The curative effects of using oxygen treatment combined mixture to bedsore
Aihua JIAN ; Guoquan LUO ; Biqiang SHEN ; Minxi HUANG
Chinese Journal of Practical Nursing 2006;0(18):-
ObjectiveTo study the curative effects of using oxygen treatment combined mixture to Ⅲ-Ⅳ bedsore. Methods Divided 63 patients into the observation group (32 cases) and the traditional group (31 cases) randomly. Oxygen treatment combined mixture was used in the observation group and the traditional nursing measures were used in the traditional group. Compared the curative effects of bedsore between the 2 groups.Results The cure rate of Ⅲ-Ⅳ bedsore in the observation group was significant higher than that of in the traditional group,P