1.A MODIFIED METHOD OF DETERMINING NK ACTIVITY IN HUMAN PERIPHERAL BLOOD:WHOLE BLOOD ASSAY
Chinese Journal of Immunology 1985;0(02):-
With use of ~(125)I-udR release test,a significant natural killer activityagainst K_(562) target cells has been determined in human peripheral wholeblood(WB).r There was a gradual decrease in NK activity as rise inthe dilution or WB.In comparison with peripheral monouclear cells(PBMC)oneself,the NK activity lowered distinctly at both 1:4 and 1:8 dilution ofWB(p0.4).It seems that neither the red blood cells nor thePlasma ffeect on NK activity of WB,The NK activity in WB as well as inPBMO was improved by incubation with human leukocyte interferon.TheNK activity of WB and PBMC in patients with hepatocellular carcinomawas signiticantly lower than in normal controls.The results showed tahtNK activity may be determined by using properly diluted WB instead orPBMC,and the assay can be easily performed in clinical practice.
2.Perioperative nursing for patients with combined endoscopic nephroureterectomy
Zhen LI ; Aihua ZHOU ; Min CHEN ; Fengqiu PENG ; Ruixing BI
Chinese Journal of Practical Nursing 2009;25(30):1-3
Objective In order to know the operative key points of combined endoscopic nephroureterectomy. Methods Retrospective analyzed 16 patients with combined endoscopic nephroureterec-tomy, and then summarized perioperative nursing points. Results All the patients had successful operation without any complications. Conclusions Careful perioperative nursing are the guarantee for successful oper-ation, which can effective promote the rehabilitation for patients.
3.ISSR Analysis on medicinal plants of Caragana Fabr. in Ordos Plateau
Jiuyan YANG ; Jie YANG ; Mingbo YANG ; Aihua JU ; Bi QU
Chinese Traditional and Herbal Drugs 1994;0(10):-
Objective To analyze the genetic diversity of eight species of Caragana Fabr. in Ordos Plateau. Methods To study the genetic diversity of the eight species with ISSR method. Results The total percentage of polymorphic bands (PPB) of the eight species was up to 100%, which was extremely high and showed an abundant genetic diversity. However, the genetic diversity of different species was not the same. The genetic diversity of C. tibetica, C. korshinskii, C. stenophylla, C. roborovskyi, and C. intermedia was higher, while that of C. opulens and C. purdomii was lower, and that of C. brachypoda was the lowest. The analytic results of the Nei′s gene diversities and the Shannon′s information index were similar. Different species could be differentiated through UPGMA method and characteristic banding pattern of ISSR. Conclusion ISSR Molecular marker could be used in molecular authentication and hereditary background study of the species of Caragana Fabr. These results provide the scientific basis of developing effective protection measures for these species.
4.Changes of distortion product otoacoustic emissions in the patients with iron deficient anaemia.
Feng SHEN ; Aihua SUN ; Qing YE ; Jie BI ; Jue CHEN ; Ting ZHANG ; Zhenxin CHEN ; Zhen FANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2008;22(19):883-885
OBJECTIVE:
To observe the changes of distortion product otoacoustic emissions (DPOAE) in the patients with iron deficient anaemia (IDA).
METHOD:
Sixty cases who were taken health physical examination at ZhaBei District Central Hospital were divided into two groups, the normal group (30 cases, 60 ears) and the IDA group (30 patients, 60 ears) depend on the results of examination of hemoglobin and serum ferritin. The changes of DPOAEs at 4 kHz were observed.
RESULT:
A statistically significant decrease of DPOAE threshold level compared with the normal controls was seen at 4 kHz in the patients with IDA.
CONCLUSION
The decreased threshold level of DPOAE at 4 kHz in IDA patients should relate to sensorineural hearing loss directly.
Adolescent
;
Adult
;
Aged
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Anemia, Iron-Deficiency
;
blood
;
physiopathology
;
Audiometry, Pure-Tone
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Auditory Threshold
;
Case-Control Studies
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Cochlea
;
physiopathology
;
Female
;
Ferritins
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blood
;
Hemoglobins
;
analysis
;
Humans
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Male
;
Middle Aged
;
Otoacoustic Emissions, Spontaneous
;
Young Adult
5.Effect of exercise on cancer-related cognitive impairment in breast cancer patients:a Meta-analysis
Yu BI ; Ping ZHU ; Yuanfeng LU ; Aihua ZHANG
Modern Clinical Nursing 2023;22(11):68-75
Objective To evaluate the intervention effect of exercise on cancer related cognitive impairment of breast cancer patients,and to provide evidence-based evidence for the choices of rehabilitation programs for cognitive impairment.Methods The PubMed,Embase,Web of Science,Cochrane Library,Joanna Briggs Institute Library(JBI),CINAHL,CNKI,Wanfang,VIP and CBM were searched by computer for randomized controlled trials on the impact of exercise on cancer-related cognitive impairment in patients with breast cancer.The retrieval time limit was from the establishment of the database to October 2022.The quality of the included studies was evaluated according to Cochrane Handbook 5.1.0.RevMan5.4 was used for meta-analysis.Results A total of 18 publications including 1310 patients were included.The results showed that exercise could improve self-reported cancer-related cognitive impairment[SMD=0.33,95%CI(0.21,0.46),P<0.001],executive function[SMD=-0.27,95%CI(-0.42,-0.11),P<0.001]and attention[SMD=-0.37,95%CI(-0.60,-0.14),P<0.01],alleviate cancer-related fatigue[SMD=-0.56,95%CI(-0.79,-0.34),P<0.001],and reduce depression[SMD=-0.73,95%CI(-1.17,-0.30),P<0.001],but it has no significant effect on the memory of patients with breast cancer[SMD=0.05,95%CI(-0.16,0.27),P=0.64].Conclusion Exercise can improve the self-reported cancer-related cognitive impairment,executive function and attention of breast cancer patients,alleviate cancer-related fatigue,reduce depression,but it has no significant effect on improving memory,which still needs further verification.