Objective To detect the change of precursor protein of sol in breast cancer before and after the neoadjuvant chemotherapy,so as to find the serum protein marker of chemotherapy drug resistance in breast cancer.Methods Breast cancer patients with Ⅲ period neoadjuvant chemotherapy were enrolled in the study.The serum specimens of the patients were obtained.Two-way gel electrophoresis was used to get the gel map before and after chemotherapy,then the image map was analyzed by gel analysis software.The differentially expressed proteins point was screened before and after chemotherapy.Serum protein differences were identified with matrix auxiliary laser disi ntegrate series time of flight mass spectrometer (MALDI-TOF-MS),including coagulation sol precursor protein.Western blot was used to test the variation trend of them before and after chemotherapy.Results Coagulation sol precursor protein levels fell after neoadjuvant chemotherapy in resistance group.The variation trend of the results of western blot and that of the proteomics were consistent.Conclusion Coagulation sol precursor protein can be as the candidate serum marker of chemotherapy drug resistance in breast cancer.

This study aims to develop a method to detect bovine multi-cytokines based on flow cytometry. Previously we have prepared and screened monoclonal antibodies against bovine cytokines IFN-γ, IL-2, TNF-α, IP-10 and MCP-1. These bovine cytokine monoclonal antibodies were fluorescently labeled, and the combination of antibody and cell surface molecules were used to develop the method for detecting bovine multi-cytokines. Subsequently, the developed method was used to determine the cytokine expression profile of Mycobacterium bovis BCG infected bovine peripheral blood mononuclear cells in vitro, and evaluate the cytokine expression level of peripheral blood CD4⁺ T cells of tuberculosis-positive cattle. The bovine multi-cytokine flow cytometry detection method can effectively determine the cytokine expression of BCG-infected bovine peripheral blood T lymphocytes. Among them, the expression levels of IFN-γ, IL-2, and TNF-α continue to increase after 40 hours of infection, while the expression levels of IP-10 and MCP-1 decreased. The combined detection of IFN-γ, IL-2, and TNF-α on CD4⁺ T lymphocytes in peripheral blood of cattle can effectively distinguish tuberculosis-positive and tuberculosis-negative samples. This method may facilitate evaluating the level of cellular immune response after bovine pathogen infection and vaccine injection.
Cattle ; Animals ; Cytokines ; BCG Vaccine/metabolism* ; Tumor Necrosis Factor-alpha/metabolism* ; Interleukin-2 ; Flow Cytometry/methods* ; Chemokine CXCL10/metabolism* ; Leukocytes, Mononuclear ; CD4-Positive T-Lymphocytes/metabolism* ; Tuberculosis ; Antibodies, Monoclonal/metabolism*